Sulfolobus ellipsoid virus 1
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 38 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H4RBQ3|A0A2H4RBQ3_9VIRU Uncharacterized protein OS=Sulfolobus ellipsoid virus 1 OX=2056194 PE=4 SV=1
MM1 pKa = 7.47 SFLSSLLKK9 pKa = 10.37 ILLSPVIPIVPKK21 pKa = 10.22 TPISTTTPVSTQTVTQLVTQTVTQVLTQYY50 pKa = 10.74 EE51 pKa = 4.63 YY52 pKa = 10.9 VYY54 pKa = 10.76 LSASCPVCPISTTVPQGYY72 pKa = 7.39 TLVYY76 pKa = 9.6 YY77 pKa = 8.44 YY78 pKa = 10.4 QISNPYY84 pKa = 9.62 SQDD87 pKa = 3.34 LNDD90 pKa = 3.3 IQVPIEE96 pKa = 3.91 LVVNNPLSYY105 pKa = 10.36 IFCEE109 pKa = 4.09 DD110 pKa = 3.39 QACNKK115 pKa = 9.74 PLFSWYY121 pKa = 9.56 EE122 pKa = 3.51 PSGIFWVLIPFIPANGDD139 pKa = 3.16 IVIYY143 pKa = 10.07 LYY145 pKa = 11.17 EE146 pKa = 5.46 NGDD149 pKa = 3.09 VNYY152 pKa = 9.35 PYY154 pKa = 10.12 TGTFGSPLYY163 pKa = 10.85 DD164 pKa = 3.18 NGRR167 pKa = 11.84 GVFYY171 pKa = 10.05 EE172 pKa = 4.07 YY173 pKa = 10.88 FSFYY177 pKa = 10.18 DD178 pKa = 3.72 TLPHH182 pKa = 6.48 EE183 pKa = 4.58 WSTCYY188 pKa = 10.51 NAVSPNYY195 pKa = 9.37 NSTYY199 pKa = 9.8 ATLPVNPPDD208 pKa = 3.82 GGIGSDD214 pKa = 3.47 YY215 pKa = 10.3 AYY217 pKa = 9.84 PVIGYY222 pKa = 8.71 WIDD225 pKa = 3.69 FYY227 pKa = 11.86 VALPSTSSSDD237 pKa = 2.97 ISSSVMALGVGSCNTTNTYY256 pKa = 10.57 FIGGGGGDD264 pKa = 3.3 TCGNNANGFAFCYY277 pKa = 10.72 DD278 pKa = 3.21 NFGTTNQWGVYY289 pKa = 9.87 VGTTQVAPSPLNYY302 pKa = 9.36 TPNNFALYY310 pKa = 10.15 SIGVLSSSIIFQMNYY325 pKa = 9.34 NNQFIYY331 pKa = 10.5 SGSPPISMGNLVILDD346 pKa = 4.08 SIGTIGLGFVRR357 pKa = 11.84 MRR359 pKa = 11.84 KK360 pKa = 8.11 ATPDD364 pKa = 3.53 GNNLPVLTFTPSGALASS381 pKa = 3.91
Molecular weight: 41.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.89
IPC2_protein 3.795
IPC_protein 3.783
Toseland 3.567
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.745
Rodwell 3.617
Grimsley 3.478
Solomon 3.77
Lehninger 3.732
Nozaki 3.91
DTASelect 4.164
Thurlkill 3.63
EMBOSS 3.757
Sillero 3.91
Patrickios 0.006
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.817
Protein with the highest isoelectric point:
>tr|A0A2H4RBQ4|A0A2H4RBQ4_9VIRU Zn_C2H2-containing protein OS=Sulfolobus ellipsoid virus 1 OX=2056194 PE=4 SV=1
MM1 pKa = 7.68 PKK3 pKa = 10.17 ANRR6 pKa = 11.84 YY7 pKa = 9.53 AGLLTISLKK16 pKa = 10.7 KK17 pKa = 10.68 LLIEE21 pKa = 4.5 LPIFDD26 pKa = 5.26 RR27 pKa = 11.84 LTLIKK32 pKa = 9.97 IRR34 pKa = 11.84 LISRR38 pKa = 11.84 VIKK41 pKa = 9.92 FHH43 pKa = 6.92 SKK45 pKa = 10.73 NNLCEE50 pKa = 4.4 RR51 pKa = 11.84 II52 pKa = 3.75
Molecular weight: 6.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.297
IPC2_protein 9.78
IPC_protein 10.321
Toseland 10.979
ProMoST 10.555
Dawson 11.023
Bjellqvist 10.687
Wikipedia 11.199
Rodwell 11.433
Grimsley 11.052
Solomon 11.155
Lehninger 11.125
Nozaki 10.95
DTASelect 10.672
Thurlkill 10.95
EMBOSS 11.359
Sillero 10.95
Patrickios 11.199
IPC_peptide 11.155
IPC2_peptide 9.516
IPC2.peptide.svr19 8.65
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
38
0
38
6917
52
814
182.0
20.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.467 ± 0.245
1.084 ± 0.155
4.409 ± 0.421
4.901 ± 0.699
4.641 ± 0.328
5.523 ± 0.405
1.128 ± 0.198
9.122 ± 0.314
6.636 ± 1.047
9.441 ± 0.502
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.183 ± 0.26
7.417 ± 0.694
4.568 ± 0.452
3.354 ± 0.421
2.732 ± 0.467
7.619 ± 0.745
6.376 ± 0.589
6.954 ± 0.395
0.853 ± 0.093
6.592 ± 0.305
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here