Gopherus associated genomovirus 1
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A384X6V0|A0A384X6V0_9VIRU Replication-associated protein OS=Gopherus associated genomovirus 1 OX=2041417 PE=3 SV=1
MM1 pKa = 7.1 FRR3 pKa = 11.84 FAARR7 pKa = 11.84 YY8 pKa = 9.22 GLLTYY13 pKa = 7.26 PQCGDD18 pKa = 3.79 LDD20 pKa = 3.44 PWAIVEE26 pKa = 4.05 MLGRR30 pKa = 11.84 LGAEE34 pKa = 4.08 CIVGRR39 pKa = 11.84 EE40 pKa = 4.01 SHH42 pKa = 6.15 QDD44 pKa = 3.23 GGVHH48 pKa = 5.67 LHH50 pKa = 6.33 AFFMFEE56 pKa = 4.48 SKK58 pKa = 10.78 FEE60 pKa = 4.17 SRR62 pKa = 11.84 NVRR65 pKa = 11.84 IFDD68 pKa = 3.59 VDD70 pKa = 3.42 GMHH73 pKa = 6.97 PNVVRR78 pKa = 11.84 GYY80 pKa = 7.6 STPEE84 pKa = 3.24 KK85 pKa = 10.21 GYY87 pKa = 11.0 AYY89 pKa = 10.04 AIKK92 pKa = 10.8 DD93 pKa = 3.37 GDD95 pKa = 4.03 FVAGGLGMEE104 pKa = 5.06 SIRR107 pKa = 11.84 RR108 pKa = 11.84 PVSEE112 pKa = 4.73 SRR114 pKa = 11.84 SVWDD118 pKa = 4.44 EE119 pKa = 3.5 IVLAGSRR126 pKa = 11.84 EE127 pKa = 4.11 EE128 pKa = 4.37 FFEE131 pKa = 4.29 ACARR135 pKa = 11.84 LAPRR139 pKa = 11.84 ALCCSFTSLNCYY151 pKa = 10.31 ADD153 pKa = 3.12 WKK155 pKa = 10.2 YY156 pKa = 10.85 RR157 pKa = 11.84 PEE159 pKa = 4.19 PVPYY163 pKa = 9.1 EE164 pKa = 3.96 HH165 pKa = 7.62 PGNVSLSTANFPEE178 pKa = 4.01 LAAWVSHH185 pKa = 5.1 NLEE188 pKa = 4.37 GLEE191 pKa = 4.65 HH192 pKa = 7.05 PGKK195 pKa = 10.23 CPPSAEE201 pKa = 4.07 EE202 pKa = 3.67 QILAIFLPTLCYY214 pKa = 10.52 GG215 pKa = 3.65
Molecular weight: 24.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.015
IPC2_protein 5.181
IPC_protein 5.118
Toseland 5.143
ProMoST 5.283
Dawson 5.143
Bjellqvist 5.258
Wikipedia 5.016
Rodwell 5.092
Grimsley 5.092
Solomon 5.143
Lehninger 5.105
Nozaki 5.296
DTASelect 5.41
Thurlkill 5.181
EMBOSS 5.105
Sillero 5.372
Patrickios 3.261
IPC_peptide 5.156
IPC2_peptide 5.385
IPC2.peptide.svr19 5.29
Protein with the highest isoelectric point:
>tr|A0A384X6W5|A0A384X6W5_9VIRU Capsid protein OS=Gopherus associated genomovirus 1 OX=2041417 PE=4 SV=1
MM1 pKa = 7.68 AYY3 pKa = 10.13 GRR5 pKa = 11.84 RR6 pKa = 11.84 SYY8 pKa = 11.16 RR9 pKa = 11.84 RR10 pKa = 11.84 TTTRR14 pKa = 11.84 RR15 pKa = 11.84 KK16 pKa = 9.36 RR17 pKa = 11.84 AGSSRR22 pKa = 11.84 RR23 pKa = 11.84 SYY25 pKa = 10.58 RR26 pKa = 11.84 RR27 pKa = 11.84 KK28 pKa = 7.6 TYY30 pKa = 7.57 TRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 TYY36 pKa = 10.14 GRR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 MSTRR44 pKa = 11.84 TVLNKK49 pKa = 9.7 TSQKK53 pKa = 10.44 KK54 pKa = 9.08 RR55 pKa = 11.84 DD56 pKa = 3.51 NMLAYY61 pKa = 10.94 SNTTFDD67 pKa = 3.97 NPFATTYY74 pKa = 9.5 TLGGAIMRR82 pKa = 11.84 FPVGNTSIPSEE93 pKa = 4.72 FIYY96 pKa = 10.53 VWNATGRR103 pKa = 11.84 PGEE106 pKa = 4.2 NSDD109 pKa = 3.54 GQRR112 pKa = 11.84 GSKK115 pKa = 10.41 LDD117 pKa = 3.38 TSLRR121 pKa = 11.84 TSEE124 pKa = 4.25 TVFARR129 pKa = 11.84 GLKK132 pKa = 10.09 EE133 pKa = 4.66 KK134 pKa = 9.54 ITLEE138 pKa = 4.33 TNNAAPWHH146 pKa = 5.36 WRR148 pKa = 11.84 RR149 pKa = 11.84 ICFTSKK155 pKa = 11.17 DD156 pKa = 3.99 DD157 pKa = 4.66 FGEE160 pKa = 4.53 ADD162 pKa = 4.63 PDD164 pKa = 3.38 TSAYY168 pKa = 9.37 FRR170 pKa = 11.84 RR171 pKa = 11.84 TSNGRR176 pKa = 11.84 VRR178 pKa = 11.84 LLRR181 pKa = 11.84 AQSDD185 pKa = 4.02 SEE187 pKa = 4.27 YY188 pKa = 11.17 LNDD191 pKa = 3.69 EE192 pKa = 4.22 LFEE195 pKa = 4.29 GEE197 pKa = 5.43 RR198 pKa = 11.84 NVDD201 pKa = 2.94 WLSALNAPLSRR212 pKa = 11.84 KK213 pKa = 9.32 HH214 pKa = 6.76 FSIKK218 pKa = 9.64 YY219 pKa = 9.32 DD220 pKa = 3.16 KK221 pKa = 10.97 TRR223 pKa = 11.84 VLRR226 pKa = 11.84 SQNQAGNIFTFNLWHH241 pKa = 6.37 NMGHH245 pKa = 6.2 NIVYY249 pKa = 10.32 AGEE252 pKa = 4.14 QTGEE256 pKa = 4.09 RR257 pKa = 11.84 MVDD260 pKa = 3.38 SAVSVSGRR268 pKa = 11.84 PGMGNYY274 pKa = 9.69 YY275 pKa = 10.5 VVDD278 pKa = 3.87 IFRR281 pKa = 11.84 KK282 pKa = 10.09 HH283 pKa = 6.63 GINDD287 pKa = 3.71 DD288 pKa = 3.55 QSTLTFTPEE297 pKa = 3.46 ATFFWHH303 pKa = 6.68 EE304 pKa = 4.05 KK305 pKa = 9.79
Molecular weight: 35.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.317
IPC2_protein 9.736
IPC_protein 10.54
Toseland 10.438
ProMoST 10.233
Dawson 10.613
Bjellqvist 10.335
Wikipedia 10.833
Rodwell 10.774
Grimsley 10.687
Solomon 10.687
Lehninger 10.643
Nozaki 10.409
DTASelect 10.335
Thurlkill 10.467
EMBOSS 10.847
Sillero 10.526
Patrickios 10.379
IPC_peptide 10.672
IPC2_peptide 9.224
IPC2.peptide.svr19 8.603
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
845
215
325
281.7
32.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.982 ± 0.643
1.893 ± 0.753
5.444 ± 0.499
6.864 ± 1.01
5.917 ± 0.304
8.639 ± 0.655
2.722 ± 0.328
3.432 ± 0.098
3.669 ± 0.493
7.456 ± 0.815
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.249 ± 0.042
4.26 ± 0.993
4.734 ± 0.885
1.775 ± 0.226
8.639 ± 1.65
7.811 ± 0.344
5.207 ± 2.141
5.68 ± 0.613
2.13 ± 0.148
4.497 ± 0.101
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here