Faba bean necrotic yellows virus associated alphasatellite 1

Taxonomy: Viruses; Alphasatellitidae; Nanoalphasatellitinae; Subclovsatellite; Faba bean necrotic yellows alphasatellite 3

Average proteome isoelectric point is 7.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D3E2A4|A0A2D3E2A4_9VIRU Replication-associated protein OS=Faba bean necrotic yellows virus associated alphasatellite 1 OX=2052606 PE=4 SV=1
MM1 pKa = 7.71ASKK4 pKa = 10.31RR5 pKa = 11.84WCFTLNYY12 pKa = 7.35KK13 pKa = 8.77TAVEE17 pKa = 4.09RR18 pKa = 11.84EE19 pKa = 4.24SFISLFTRR27 pKa = 11.84DD28 pKa = 3.52EE29 pKa = 3.89INYY32 pKa = 7.21FVCGDD37 pKa = 3.47EE38 pKa = 4.38TAPTTYY44 pKa = 10.06QKK46 pKa = 10.77HH47 pKa = 4.68LQGYY51 pKa = 8.3VSFKK55 pKa = 11.08KK56 pKa = 9.93MIRR59 pKa = 11.84LGGLKK64 pKa = 10.18KK65 pKa = 10.61KK66 pKa = 10.51FGSRR70 pKa = 11.84AHH72 pKa = 6.01WEE74 pKa = 3.74IAKK77 pKa = 10.64GDD79 pKa = 3.76DD80 pKa = 3.53FQNRR84 pKa = 11.84DD85 pKa = 3.34YY86 pKa = 10.29CTKK89 pKa = 8.9EE90 pKa = 3.85TLISEE95 pKa = 4.16IGAPVKK101 pKa = 10.54KK102 pKa = 10.49GSNQRR107 pKa = 11.84KK108 pKa = 9.46IMDD111 pKa = 5.36LYY113 pKa = 11.08LQDD116 pKa = 4.11PEE118 pKa = 4.52EE119 pKa = 4.36MQLKK123 pKa = 10.83DD124 pKa = 3.36PDD126 pKa = 3.57TALRR130 pKa = 11.84CNAKK134 pKa = 9.9KK135 pKa = 10.52LRR137 pKa = 11.84IEE139 pKa = 4.23YY140 pKa = 10.26CSSFDD145 pKa = 4.09VLSLRR150 pKa = 11.84PWQSEE155 pKa = 3.62LHH157 pKa = 5.4RR158 pKa = 11.84VLMTEE163 pKa = 3.83PDD165 pKa = 3.74DD166 pKa = 3.79RR167 pKa = 11.84TIIWVYY173 pKa = 10.79GPDD176 pKa = 3.46GGEE179 pKa = 4.18GKK181 pKa = 8.39STFAKK186 pKa = 10.41EE187 pKa = 3.8LIKK190 pKa = 10.99YY191 pKa = 8.62GWFYY195 pKa = 11.64NPGGKK200 pKa = 7.47TQDD203 pKa = 3.18ILYY206 pKa = 8.78MYY208 pKa = 10.51AQDD211 pKa = 3.86PEE213 pKa = 4.49RR214 pKa = 11.84NIAFDD219 pKa = 3.62VPRR222 pKa = 11.84CSSEE226 pKa = 3.66MMNYY230 pKa = 9.83QAMEE234 pKa = 3.91MLKK237 pKa = 10.26NRR239 pKa = 11.84VFASTKK245 pKa = 9.34YY246 pKa = 10.33RR247 pKa = 11.84PVDD250 pKa = 3.23LCVRR254 pKa = 11.84RR255 pKa = 11.84KK256 pKa = 8.58VHH258 pKa = 7.03LIVFANVSPDD268 pKa = 3.29PTKK271 pKa = 10.38ISEE274 pKa = 4.02DD275 pKa = 3.5RR276 pKa = 11.84IVIVNCC282 pKa = 4.12

Molecular weight: 32.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D3E2A4|A0A2D3E2A4_9VIRU Replication-associated protein OS=Faba bean necrotic yellows virus associated alphasatellite 1 OX=2052606 PE=4 SV=1
MM1 pKa = 7.71ASKK4 pKa = 10.31RR5 pKa = 11.84WCFTLNYY12 pKa = 7.35KK13 pKa = 8.77TAVEE17 pKa = 4.09RR18 pKa = 11.84EE19 pKa = 4.24SFISLFTRR27 pKa = 11.84DD28 pKa = 3.52EE29 pKa = 3.89INYY32 pKa = 7.21FVCGDD37 pKa = 3.47EE38 pKa = 4.38TAPTTYY44 pKa = 10.06QKK46 pKa = 10.77HH47 pKa = 4.68LQGYY51 pKa = 8.3VSFKK55 pKa = 11.08KK56 pKa = 9.93MIRR59 pKa = 11.84LGGLKK64 pKa = 10.18KK65 pKa = 10.61KK66 pKa = 10.51FGSRR70 pKa = 11.84AHH72 pKa = 6.01WEE74 pKa = 3.74IAKK77 pKa = 10.64GDD79 pKa = 3.76DD80 pKa = 3.53FQNRR84 pKa = 11.84DD85 pKa = 3.34YY86 pKa = 10.29CTKK89 pKa = 8.9EE90 pKa = 3.85TLISEE95 pKa = 4.16IGAPVKK101 pKa = 10.54KK102 pKa = 10.49GSNQRR107 pKa = 11.84KK108 pKa = 9.46IMDD111 pKa = 5.36LYY113 pKa = 11.08LQDD116 pKa = 4.11PEE118 pKa = 4.52EE119 pKa = 4.36MQLKK123 pKa = 10.83DD124 pKa = 3.36PDD126 pKa = 3.57TALRR130 pKa = 11.84CNAKK134 pKa = 9.9KK135 pKa = 10.52LRR137 pKa = 11.84IEE139 pKa = 4.23YY140 pKa = 10.26CSSFDD145 pKa = 4.09VLSLRR150 pKa = 11.84PWQSEE155 pKa = 3.62LHH157 pKa = 5.4RR158 pKa = 11.84VLMTEE163 pKa = 3.83PDD165 pKa = 3.74DD166 pKa = 3.79RR167 pKa = 11.84TIIWVYY173 pKa = 10.79GPDD176 pKa = 3.46GGEE179 pKa = 4.18GKK181 pKa = 8.39STFAKK186 pKa = 10.41EE187 pKa = 3.8LIKK190 pKa = 10.99YY191 pKa = 8.62GWFYY195 pKa = 11.64NPGGKK200 pKa = 7.47TQDD203 pKa = 3.18ILYY206 pKa = 8.78MYY208 pKa = 10.51AQDD211 pKa = 3.86PEE213 pKa = 4.49RR214 pKa = 11.84NIAFDD219 pKa = 3.62VPRR222 pKa = 11.84CSSEE226 pKa = 3.66MMNYY230 pKa = 9.83QAMEE234 pKa = 3.91MLKK237 pKa = 10.26NRR239 pKa = 11.84VFASTKK245 pKa = 9.34YY246 pKa = 10.33RR247 pKa = 11.84PVDD250 pKa = 3.23LCVRR254 pKa = 11.84RR255 pKa = 11.84KK256 pKa = 8.58VHH258 pKa = 7.03LIVFANVSPDD268 pKa = 3.29PTKK271 pKa = 10.38ISEE274 pKa = 4.02DD275 pKa = 3.5RR276 pKa = 11.84IVIVNCC282 pKa = 4.12

Molecular weight: 32.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

282

282

282

282.0

32.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.965 ± 0.0

2.837 ± 0.0

6.738 ± 0.0

6.383 ± 0.0

4.61 ± 0.0

5.319 ± 0.0

1.418 ± 0.0

6.028 ± 0.0

8.511 ± 0.0

7.092 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.546 ± 0.0

3.901 ± 0.0

4.61 ± 0.0

3.546 ± 0.0

6.738 ± 0.0

6.028 ± 0.0

5.319 ± 0.0

5.674 ± 0.0

1.773 ± 0.0

4.965 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski