Escherichia phage 13a

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Teseptimavirus; Escherichia virus 13a

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B3VD31|B3VD31_9CAUD Gp0.4 OS=Escherichia phage 13a OX=532076 GN=0.4 PE=4 SV=1
MM1 pKa = 7.48AMSNMTYY8 pKa = 11.18NNVFDD13 pKa = 4.72HH14 pKa = 6.93AYY16 pKa = 10.49EE17 pKa = 3.86MLKK20 pKa = 10.85EE21 pKa = 4.08NIRR24 pKa = 11.84YY25 pKa = 9.81DD26 pKa = 4.67DD27 pKa = 4.5IRR29 pKa = 11.84DD30 pKa = 3.55TDD32 pKa = 4.04DD33 pKa = 3.27LHH35 pKa = 7.85DD36 pKa = 5.9AIHH39 pKa = 6.3EE40 pKa = 4.35AADD43 pKa = 3.52NAVPHH48 pKa = 6.38YY49 pKa = 9.99YY50 pKa = 10.67SDD52 pKa = 3.44IFSVMASEE60 pKa = 5.41GIYY63 pKa = 10.52HH64 pKa = 6.48EE65 pKa = 5.16FEE67 pKa = 5.33DD68 pKa = 4.72SGLMPDD74 pKa = 3.68TKK76 pKa = 10.9DD77 pKa = 3.13VTRR80 pKa = 11.84ILQARR85 pKa = 11.84IYY87 pKa = 9.25EE88 pKa = 4.14QLTIDD93 pKa = 3.64LWEE96 pKa = 4.69DD97 pKa = 3.57AEE99 pKa = 4.86DD100 pKa = 4.13LLNEE104 pKa = 3.98YY105 pKa = 10.52LEE107 pKa = 4.29EE108 pKa = 4.24VEE110 pKa = 5.64EE111 pKa = 4.29EE112 pKa = 4.34EE113 pKa = 5.01EE114 pKa = 4.49DD115 pKa = 3.82LL116 pKa = 5.45

Molecular weight: 13.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B3VD52|B3VD52_9CAUD Gp4.7 OS=Escherichia phage 13a OX=532076 GN=4.7 PE=4 SV=1
MM1 pKa = 7.43SNVAEE6 pKa = 4.74TIRR9 pKa = 11.84LSDD12 pKa = 4.91TADD15 pKa = 2.65QWNRR19 pKa = 11.84RR20 pKa = 11.84VHH22 pKa = 6.18INVRR26 pKa = 11.84NGKK29 pKa = 8.54ATMVYY34 pKa = 9.07RR35 pKa = 11.84WKK37 pKa = 10.58DD38 pKa = 3.37SKK40 pKa = 11.14SSKK43 pKa = 9.98NHH45 pKa = 4.44TQRR48 pKa = 11.84MTLTDD53 pKa = 3.75EE54 pKa = 3.92QALRR58 pKa = 11.84LVNALTIAAVTAIHH72 pKa = 5.41EE73 pKa = 4.38TGRR76 pKa = 11.84VNEE79 pKa = 4.17AMAIIDD85 pKa = 3.52KK86 pKa = 9.46YY87 pKa = 11.0LRR89 pKa = 11.84IRR91 pKa = 11.84VVV93 pKa = 2.73

Molecular weight: 10.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

12576

30

1318

228.7

25.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.739 ± 0.484

1.272 ± 0.217

6.33 ± 0.281

7.125 ± 0.354

3.467 ± 0.2

7.483 ± 0.318

2.083 ± 0.218

5.304 ± 0.184

6.632 ± 0.328

8.182 ± 0.328

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.125 ± 0.247

4.461 ± 0.328

3.356 ± 0.22

3.729 ± 0.326

5.685 ± 0.212

6.099 ± 0.324

5.55 ± 0.238

6.393 ± 0.285

1.606 ± 0.193

3.379 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski