Pseudomonas phage LUZ7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Migulavirinae; Luzseptimavirus; Pseudomonas virus LUZ7

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 115 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C8ZKE5|C8ZKE5_9CAUD Uncharacterized protein OS=Pseudomonas phage LUZ7 OX=655097 PE=4 SV=1
MM1 pKa = 7.28SKK3 pKa = 10.44SYY5 pKa = 9.66TVVFNTDD12 pKa = 2.59AVEE15 pKa = 3.92QLGSFIQAMKK25 pKa = 10.64QEE27 pKa = 4.73GIEE30 pKa = 4.24VSALCQGDD38 pKa = 4.71AILKK42 pKa = 10.41AEE44 pKa = 4.23ALVHH48 pKa = 5.73YY49 pKa = 8.69FPAEE53 pKa = 3.78LTPEE57 pKa = 4.27VIEE60 pKa = 4.59GFCNKK65 pKa = 8.6YY66 pKa = 10.42QEE68 pKa = 4.3MEE70 pKa = 4.55DD71 pKa = 3.62YY72 pKa = 10.63HH73 pKa = 8.55VSQQEE78 pKa = 4.19EE79 pKa = 4.57IVV81 pKa = 3.38

Molecular weight: 9.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C8ZKA7|C8ZKA7_9CAUD Uncharacterized protein OS=Pseudomonas phage LUZ7 OX=655097 PE=4 SV=1
MM1 pKa = 6.82TTFTLEE7 pKa = 3.72NQLFVVRR14 pKa = 11.84LMPTKK19 pKa = 10.59GKK21 pKa = 10.74LEE23 pKa = 4.26GYY25 pKa = 9.47RR26 pKa = 11.84ICLTLIKK33 pKa = 10.44GKK35 pKa = 9.36EE36 pKa = 3.98RR37 pKa = 11.84QVLVHH42 pKa = 5.79EE43 pKa = 4.4QMFGRR48 pKa = 11.84RR49 pKa = 11.84EE50 pKa = 3.84DD51 pKa = 3.62AMHH54 pKa = 5.93VRR56 pKa = 11.84SLITARR62 pKa = 11.84RR63 pKa = 11.84VLNLKK68 pKa = 9.44HH69 pKa = 5.86WVWDD73 pKa = 3.71AKK75 pKa = 11.49SNFFGDD81 pKa = 4.02ALAHH85 pKa = 6.44KK86 pKa = 7.67PTARR90 pKa = 11.84FRR92 pKa = 11.84TKK94 pKa = 10.34PIYY97 pKa = 10.6LSFF100 pKa = 5.25

Molecular weight: 11.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

115

0

115

23817

35

3403

207.1

23.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.245 ± 0.621

0.949 ± 0.158

5.719 ± 0.159

6.995 ± 0.273

3.611 ± 0.143

6.684 ± 0.28

2.015 ± 0.182

4.875 ± 0.212

6.08 ± 0.254

9.199 ± 0.279

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.141 ± 0.113

4.539 ± 0.154

3.875 ± 0.21

4.392 ± 0.264

5.324 ± 0.166

5.996 ± 0.164

5.685 ± 0.199

6.882 ± 0.161

1.402 ± 0.154

3.393 ± 0.257
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski