Wellfleet Bay virus
Average proteome isoelectric point is 7.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A1E6X3|A0A0A1E6X3_9ORTO RNA-directed RNA polymerase catalytic subunit OS=Wellfleet Bay virus OX=1566309 GN=PB1 PE=4 SV=1
MM1 pKa = 7.35 AFEE4 pKa = 4.77 VYY6 pKa = 10.13 RR7 pKa = 11.84 LAISNPILYY16 pKa = 9.92 DD17 pKa = 3.35 PRR19 pKa = 11.84 FVSVAGEE26 pKa = 4.12 NSEE29 pKa = 3.99 HH30 pKa = 6.73 WPRR33 pKa = 11.84 DD34 pKa = 3.06 NWKK37 pKa = 10.16 KK38 pKa = 10.38 RR39 pKa = 11.84 EE40 pKa = 3.66 EE41 pKa = 4.3 SLRR44 pKa = 11.84 HH45 pKa = 5.62 DD46 pKa = 3.85 QVCALLMNTASRR58 pKa = 11.84 FPSHH62 pKa = 6.97 LEE64 pKa = 3.49 AAKK67 pKa = 10.19 EE68 pKa = 4.15 SSSSKK73 pKa = 8.95 SQIYY77 pKa = 10.23 EE78 pKa = 3.86 EE79 pKa = 4.98 GEE81 pKa = 3.88 KK82 pKa = 10.54 QFNQEE87 pKa = 4.04 EE88 pKa = 4.59 IEE90 pKa = 4.17 GLMEE94 pKa = 5.28 AGQMHH99 pKa = 6.72 LTEE102 pKa = 4.69 SFSTSVRR109 pKa = 11.84 QDD111 pKa = 3.43 DD112 pKa = 4.07 EE113 pKa = 4.64 EE114 pKa = 4.32 EE115 pKa = 4.53 SEE117 pKa = 4.67 GEE119 pKa = 4.15 GGEE122 pKa = 4.53 SGDD125 pKa = 5.41 DD126 pKa = 3.67 EE127 pKa = 6.4 DD128 pKa = 6.53 DD129 pKa = 3.45 EE130 pKa = 5.15 FAIGDD135 pKa = 3.46 YY136 pKa = 9.71 WGPPEE141 pKa = 3.91 QTEE144 pKa = 4.24 EE145 pKa = 4.11 EE146 pKa = 4.34 MEE148 pKa = 3.98 EE149 pKa = 4.17 MINVGEE155 pKa = 4.25 PDD157 pKa = 4.43 LVDD160 pKa = 3.22 SDD162 pKa = 3.4 KK163 pKa = 11.12 RR164 pKa = 11.84 YY165 pKa = 8.65 TILEE169 pKa = 4.27 GVTGAKK175 pKa = 8.03 YY176 pKa = 7.98 TQIAFSTLWGIDD188 pKa = 3.33 TQKK191 pKa = 10.37 QWDD194 pKa = 4.14 IIDD197 pKa = 3.51 KK198 pKa = 9.13 QEE200 pKa = 3.77 RR201 pKa = 11.84 KK202 pKa = 9.79 FIEE205 pKa = 4.05 VKK207 pKa = 8.55 ITSRR211 pKa = 11.84 NPYY214 pKa = 8.65 QVWEE218 pKa = 4.28 EE219 pKa = 4.12 VKK221 pKa = 10.39 SASDD225 pKa = 2.86 ISEE228 pKa = 4.05 EE229 pKa = 4.12 HH230 pKa = 6.23 FGACIVHH237 pKa = 7.09 DD238 pKa = 4.17 EE239 pKa = 4.62 GGGSFKK245 pKa = 10.88 QYY247 pKa = 10.43 VFNIDD252 pKa = 4.27 EE253 pKa = 4.3 MPGWKK258 pKa = 9.32 QVEE261 pKa = 4.23 DD262 pKa = 3.82 FLIRR266 pKa = 11.84 RR267 pKa = 11.84 YY268 pKa = 10.42 EE269 pKa = 3.84 FFLEE273 pKa = 4.16 NGAVPVAGGDD283 pKa = 3.54 EE284 pKa = 3.98 DD285 pKa = 5.8 RR286 pKa = 11.84 YY287 pKa = 10.21 PVWDD291 pKa = 4.72 PIISEE296 pKa = 5.02 KK297 pKa = 9.68 IHH299 pKa = 7.04 DD300 pKa = 4.85 WIHH303 pKa = 6.53 PLWQPDD309 pKa = 3.93 KK310 pKa = 11.18 INARR314 pKa = 11.84 VDD316 pKa = 3.06 NSPRR320 pKa = 11.84 YY321 pKa = 8.87 EE322 pKa = 4.02 PFNIGLFLNLLDD334 pKa = 5.09 DD335 pKa = 4.24 PRR337 pKa = 11.84 ARR339 pKa = 11.84 NCSKK343 pKa = 9.93 SAKK346 pKa = 9.16 WNGKK350 pKa = 9.19 LMPFSWNSAVATTCDD365 pKa = 3.41 KK366 pKa = 11.27 DD367 pKa = 4.0 LDD369 pKa = 3.98 MVEE372 pKa = 4.09 EE373 pKa = 4.8 IIRR376 pKa = 11.84 HH377 pKa = 5.39 FDD379 pKa = 3.47 CEE381 pKa = 4.18 SWSVVNWKK389 pKa = 9.03 EE390 pKa = 3.73 QSAEE394 pKa = 4.05 SLVNGLKK401 pKa = 10.76 LLLEE405 pKa = 4.22 RR406 pKa = 11.84 FKK408 pKa = 11.42 EE409 pKa = 4.31 GFIEE413 pKa = 4.26 RR414 pKa = 11.84 NSYY417 pKa = 10.56 IPVKK421 pKa = 9.04 KK422 pKa = 8.59 TKK424 pKa = 10.44 HH425 pKa = 4.16 GWTVKK430 pKa = 10.36 GEE432 pKa = 4.02 RR433 pKa = 11.84 HH434 pKa = 4.55 TSEE437 pKa = 3.79 IQEE440 pKa = 4.22 MLSEE444 pKa = 5.35 LGIGWKK450 pKa = 10.04 FYY452 pKa = 9.88 KK453 pKa = 9.37 HH454 pKa = 5.42 TGIVEE459 pKa = 3.84 EE460 pKa = 5.39 DD461 pKa = 3.08 MRR463 pKa = 11.84 QPEE466 pKa = 4.34 SEE468 pKa = 4.36 DD469 pKa = 3.41 QEE471 pKa = 4.27 MMRR474 pKa = 11.84 WDD476 pKa = 3.58 DD477 pKa = 3.25 WLTEE481 pKa = 3.95 LRR483 pKa = 11.84 ISEE486 pKa = 4.31 SQGLQGGRR494 pKa = 11.84 VFKK497 pKa = 11.03 DD498 pKa = 3.41 DD499 pKa = 3.65 VFKK502 pKa = 10.59 EE503 pKa = 4.15 APSFHH508 pKa = 7.44 ILDD511 pKa = 3.97 EE512 pKa = 4.22 PAKK515 pKa = 10.12 RR516 pKa = 11.84 ICTEE520 pKa = 3.68 LFDD523 pKa = 5.33 LFRR526 pKa = 11.84 THH528 pKa = 7.78 KK529 pKa = 10.48 IGATCAKK536 pKa = 9.95 VMNFYY541 pKa = 11.19 SRR543 pKa = 11.84 MGGTYY548 pKa = 10.15 LRR550 pKa = 11.84 AANGGKK556 pKa = 9.97 SKK558 pKa = 8.91 MHH560 pKa = 6.63 SSLAILPLYY569 pKa = 8.79 YY570 pKa = 9.91 KK571 pKa = 9.79 EE572 pKa = 4.02 YY573 pKa = 10.68 RR574 pKa = 11.84 GDD576 pKa = 3.75 RR577 pKa = 11.84 EE578 pKa = 4.15 EE579 pKa = 4.18 KK580 pKa = 8.1 TSQRR584 pKa = 11.84 VMVGFVVRR592 pKa = 11.84 GPHH595 pKa = 5.89 HH596 pKa = 6.92 VRR598 pKa = 11.84 DD599 pKa = 3.84 ATDD602 pKa = 4.13 TINLIIAEE610 pKa = 4.38 KK611 pKa = 10.74 VNLSLEE617 pKa = 4.05 EE618 pKa = 3.98 CKK620 pKa = 10.29 RR621 pKa = 11.84 RR622 pKa = 11.84 LRR624 pKa = 11.84 GGVLTSSGWFIRR636 pKa = 11.84 KK637 pKa = 8.47 NAIRR641 pKa = 11.84 KK642 pKa = 9.1 ADD644 pKa = 3.57 PTYY647 pKa = 11.2 LSFLHH652 pKa = 6.59 NSLFVPVNFLGEE664 pKa = 5.04 LITNHH669 pKa = 5.73 PQRR672 pKa = 11.84 SRR674 pKa = 11.84 TKK676 pKa = 10.18 EE677 pKa = 3.73 DD678 pKa = 2.88 PKK680 pKa = 11.01 YY681 pKa = 10.2 WDD683 pKa = 4.11 VILAGCLTSCRR694 pKa = 11.84 PFHH697 pKa = 7.06 LEE699 pKa = 3.96 RR700 pKa = 11.84 IVEE703 pKa = 4.24 TVLMGIIGKK712 pKa = 7.75 SQEE715 pKa = 4.04 EE716 pKa = 5.61 GYY718 pKa = 10.96 HH719 pKa = 6.41 DD720 pKa = 4.5 MLRR723 pKa = 11.84 KK724 pKa = 10.15 VYY726 pKa = 9.0 MLLMARR732 pKa = 11.84 GRR734 pKa = 11.84 GEE736 pKa = 3.54 IALVMDD742 pKa = 4.93 LKK744 pKa = 11.69 AMAEE748 pKa = 4.05 EE749 pKa = 4.22 MNEE752 pKa = 3.93 CLINSAYY759 pKa = 10.01 VFWVHH764 pKa = 7.2 CEE766 pKa = 3.79 LLDD769 pKa = 3.9 FMKK772 pKa = 10.39 FVKK775 pKa = 10.5 DD776 pKa = 3.06 KK777 pKa = 11.13 TPFNEE782 pKa = 4.19 HH783 pKa = 5.99 LL784 pKa = 3.95
Molecular weight: 90.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.047
IPC2_protein 5.041
IPC_protein 5.016
Toseland 4.965
ProMoST 5.143
Dawson 4.991
Bjellqvist 5.118
Wikipedia 4.851
Rodwell 4.914
Grimsley 4.889
Solomon 4.991
Lehninger 4.952
Nozaki 5.118
DTASelect 5.232
Thurlkill 4.952
EMBOSS 4.889
Sillero 5.194
Patrickios 4.126
IPC_peptide 5.003
IPC2_peptide 5.194
IPC2.peptide.svr19 5.127
Protein with the highest isoelectric point:
>tr|A0A0A1E760|A0A0A1E760_9ORTO Matrix protein OS=Wellfleet Bay virus OX=1566309 GN=M PE=4 SV=1
MM1 pKa = 6.9 QASPEE6 pKa = 4.28 AIQARR11 pKa = 11.84 KK12 pKa = 9.32 EE13 pKa = 4.02 RR14 pKa = 11.84 LLKK17 pKa = 10.4 LARR20 pKa = 11.84 LVNQVAEE27 pKa = 4.45 EE28 pKa = 4.28 EE29 pKa = 4.32 NGHH32 pKa = 7.08 DD33 pKa = 4.9 VLNLLKK39 pKa = 10.54 KK40 pKa = 10.71 EE41 pKa = 4.05 PVCNKK46 pKa = 10.01 RR47 pKa = 11.84 VITKK51 pKa = 7.44 YY52 pKa = 10.65 ARR54 pKa = 11.84 VVKK57 pKa = 10.51 DD58 pKa = 3.76 PDD60 pKa = 5.1 PIATTQLLMGQKK72 pKa = 10.23 FPILAKK78 pKa = 10.2 KK79 pKa = 10.46 SYY81 pKa = 10.03 LRR83 pKa = 11.84 YY84 pKa = 7.68 YY85 pKa = 9.96 TEE87 pKa = 4.23 AEE89 pKa = 4.01 RR90 pKa = 11.84 EE91 pKa = 4.1 EE92 pKa = 5.09 LFSNDD97 pKa = 2.96 DD98 pKa = 3.52 CRR100 pKa = 11.84 RR101 pKa = 11.84 VGWVKK106 pKa = 10.63 CSRR109 pKa = 11.84 KK110 pKa = 10.28 AIDD113 pKa = 3.14 LWISKK118 pKa = 8.88 GKK120 pKa = 9.42 PLTEE124 pKa = 4.02 EE125 pKa = 3.42 QRR127 pKa = 11.84 QIIKK131 pKa = 10.22 VLYY134 pKa = 8.88 EE135 pKa = 3.99 SSIEE139 pKa = 4.03 MAKK142 pKa = 10.59 DD143 pKa = 4.3 FYY145 pKa = 11.52 SRR147 pKa = 11.84 AWDD150 pKa = 3.68 RR151 pKa = 11.84 ATVTYY156 pKa = 10.63 GRR158 pKa = 11.84 VPLEE162 pKa = 4.13 RR163 pKa = 11.84 QSVATRR169 pKa = 11.84 NVLVNVPRR177 pKa = 11.84 EE178 pKa = 4.04 YY179 pKa = 9.96 RR180 pKa = 11.84 QKK182 pKa = 10.75 AVVEE186 pKa = 4.26 IVQPGYY192 pKa = 10.96 NLNQPEE198 pKa = 4.22 VKK200 pKa = 9.82 IYY202 pKa = 9.85 VQQILEE208 pKa = 4.54 KK209 pKa = 9.13 IGQRR213 pKa = 11.84 IVTGMSIVDD222 pKa = 3.44 QARR225 pKa = 11.84 ILMNSLDD232 pKa = 3.94 PKK234 pKa = 10.71 RR235 pKa = 11.84 RR236 pKa = 11.84 ILPVALSLHH245 pKa = 6.44 GEE247 pKa = 4.52 LAQHH251 pKa = 6.84 SIAYY255 pKa = 9.33 YY256 pKa = 9.58 GNNWMITQLPGRR268 pKa = 11.84 TYY270 pKa = 9.56 STPGDD275 pKa = 3.81 TYY277 pKa = 11.64 DD278 pKa = 3.5 MRR280 pKa = 11.84 RR281 pKa = 11.84 ICAFILSRR289 pKa = 11.84 ISKK292 pKa = 10.62 LEE294 pKa = 3.94 LNRR297 pKa = 11.84 KK298 pKa = 9.4 RR299 pKa = 11.84 EE300 pKa = 3.9 ALGRR304 pKa = 11.84 LKK306 pKa = 10.51 RR307 pKa = 11.84 GKK309 pKa = 10.13 EE310 pKa = 3.78 PFIEE314 pKa = 4.82 TIKK317 pKa = 9.87 KK318 pKa = 7.94 TPEE321 pKa = 4.02 DD322 pKa = 3.68 YY323 pKa = 10.59 AVKK326 pKa = 10.13 VIKK329 pKa = 10.48 SIMRR333 pKa = 11.84 LPCSRR338 pKa = 11.84 KK339 pKa = 8.16 HH340 pKa = 6.94 DD341 pKa = 3.87 YY342 pKa = 10.78 FGTEE346 pKa = 3.98 MIVSKK351 pKa = 10.49 KK352 pKa = 10.58 GPNTEE357 pKa = 4.34 VVTHH361 pKa = 6.53 PSGIQWRR368 pKa = 11.84 EE369 pKa = 3.67 YY370 pKa = 9.68 IDD372 pKa = 3.38 EE373 pKa = 4.03 EE374 pKa = 4.54 KK375 pKa = 10.43 IYY377 pKa = 10.31 FRR379 pKa = 11.84 HH380 pKa = 5.94 LEE382 pKa = 3.87 CRR384 pKa = 11.84 GWFTHH389 pKa = 6.21 NRR391 pKa = 11.84 ATLTEE396 pKa = 4.04 ITFSITDD403 pKa = 4.5 RR404 pKa = 11.84 ITFEE408 pKa = 3.96 NLLVNIANYY417 pKa = 9.48 IRR419 pKa = 11.84 YY420 pKa = 9.18 DD421 pKa = 3.08 WVYY424 pKa = 11.04 RR425 pKa = 11.84 PGKK428 pKa = 9.62 SIKK431 pKa = 8.38 EE432 pKa = 3.95 LRR434 pKa = 11.84 DD435 pKa = 3.27 LTMLEE440 pKa = 4.4 VKK442 pKa = 10.46 KK443 pKa = 10.51 EE444 pKa = 3.74 PWKK447 pKa = 10.79 ILGTNKK453 pKa = 9.99 RR454 pKa = 11.84 VWEE457 pKa = 4.39 EE458 pKa = 3.68 YY459 pKa = 8.68 WRR461 pKa = 11.84 HH462 pKa = 6.36 IEE464 pKa = 4.91 DD465 pKa = 3.65 SLITITDD472 pKa = 3.15 EE473 pKa = 3.9 MKK475 pKa = 10.76 NYY477 pKa = 8.75 LTFEE481 pKa = 4.14 SKK483 pKa = 10.83 VNVRR487 pKa = 11.84 CVYY490 pKa = 9.12 KK491 pKa = 10.43 TEE493 pKa = 4.11 IPEE496 pKa = 4.05 EE497 pKa = 4.47 TEE499 pKa = 3.25 VHH501 pKa = 5.81 YY502 pKa = 9.93 EE503 pKa = 3.83 VRR505 pKa = 11.84 PDD507 pKa = 3.45 GSLEE511 pKa = 4.21 EE512 pKa = 4.06 IDD514 pKa = 3.82 GNSRR518 pKa = 11.84 IPYY521 pKa = 9.74 IEE523 pKa = 4.58 IKK525 pKa = 9.34 TAGIPHH531 pKa = 7.7 LDD533 pKa = 3.6 MTWNSNDD540 pKa = 3.24 MYY542 pKa = 10.31 IIPLLHH548 pKa = 6.35 PQISMMRR555 pKa = 11.84 TIEE558 pKa = 4.01 FHH560 pKa = 6.78 LLRR563 pKa = 11.84 PNLEE567 pKa = 3.75 EE568 pKa = 7.73 DD569 pKa = 2.9 IMKK572 pKa = 10.92 NNFSTHH578 pKa = 5.78 NAHH581 pKa = 6.55 CPYY584 pKa = 9.42 WLEE587 pKa = 4.14 AASMAGFCNQARR599 pKa = 11.84 MMLYY603 pKa = 10.71 GAMFKK608 pKa = 10.84 LKK610 pKa = 9.43 TIPKK614 pKa = 9.36 IFLAYY619 pKa = 9.81 LYY621 pKa = 9.91 CFSGYY626 pKa = 9.41 PRR628 pKa = 11.84 AHH630 pKa = 5.76 TKK632 pKa = 9.62 YY633 pKa = 10.57 NEE635 pKa = 4.08 RR636 pKa = 11.84 ARR638 pKa = 11.84 MKK640 pKa = 10.34 TGGFLDD646 pKa = 5.79 LYY648 pKa = 11.11 AKK650 pKa = 10.48 DD651 pKa = 3.73 GNFRR655 pKa = 11.84 RR656 pKa = 11.84 DD657 pKa = 3.35 PEE659 pKa = 4.26 TGKK662 pKa = 9.59 WLIFDD667 pKa = 4.35 KK668 pKa = 10.76 EE669 pKa = 4.43 WVGVVTDD676 pKa = 4.33 MGPEE680 pKa = 4.15 PEE682 pKa = 4.03 LLAYY686 pKa = 8.78 TFLNGFRR693 pKa = 11.84 LEE695 pKa = 4.29 SVKK698 pKa = 10.27 KK699 pKa = 10.46 RR700 pKa = 11.84 KK701 pKa = 9.42 GDD703 pKa = 3.26 HH704 pKa = 6.1 GLANLRR710 pKa = 11.84 EE711 pKa = 4.14 GMEE714 pKa = 4.13 ALKK717 pKa = 10.37 EE718 pKa = 4.15 KK719 pKa = 10.56 QDD721 pKa = 3.37 GEE723 pKa = 4.38 MIYY726 pKa = 10.94 VRR728 pKa = 11.84 IGGDD732 pKa = 3.12 VKK734 pKa = 11.19 LLVRR738 pKa = 11.84 DD739 pKa = 3.58 RR740 pKa = 11.84 TTDD743 pKa = 2.87 AKK745 pKa = 10.51 RR746 pKa = 11.84 ALAIKK751 pKa = 10.2 RR752 pKa = 11.84 GRR754 pKa = 11.84 EE755 pKa = 3.36 RR756 pKa = 11.84 VLEE759 pKa = 4.17 DD760 pKa = 3.93 RR761 pKa = 11.84 EE762 pKa = 4.35 LEE764 pKa = 4.25 LMSSLQPKK772 pKa = 8.83 RR773 pKa = 11.84 AKK775 pKa = 10.5 KK776 pKa = 9.39 EE777 pKa = 3.74
Molecular weight: 90.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.686
IPC2_protein 8.624
IPC_protein 8.507
Toseland 9.151
ProMoST 9.019
Dawson 9.487
Bjellqvist 9.268
Wikipedia 9.663
Rodwell 9.692
Grimsley 9.56
Solomon 9.502
Lehninger 9.458
Nozaki 9.341
DTASelect 9.209
Thurlkill 9.326
EMBOSS 9.619
Sillero 9.458
Patrickios 4.749
IPC_peptide 9.502
IPC2_peptide 7.878
IPC2.peptide.svr19 7.725
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
3767
128
784
538.1
61.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.053 ± 0.405
1.752 ± 0.339
5.256 ± 0.38
8.097 ± 0.749
3.955 ± 0.32
5.946 ± 0.338
2.177 ± 0.194
5.946 ± 0.328
7.008 ± 0.321
8.866 ± 0.436
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.318 ± 0.351
4.831 ± 0.406
4.088 ± 0.247
3.132 ± 0.205
6.291 ± 0.759
6.822 ± 0.547
5.177 ± 0.473
6.053 ± 0.235
1.805 ± 0.247
3.424 ± 0.314
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here