Thalassobaculum litoreum DSM 18839
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4959 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G7RJU1|A0A1G7RJU1_9PROT Probable phosphoglycerate mutase OS=Thalassobaculum litoreum DSM 18839 OX=1123362 GN=SAMN05660686_03409 PE=4 SV=1
MM1 pKa = 7.17 TVIPSDD7 pKa = 4.29 PLFGDD12 pKa = 2.72 QWYY15 pKa = 9.28 LHH17 pKa = 5.81 NTGQSGGTVGQDD29 pKa = 2.68 IRR31 pKa = 11.84 VLGAWPDD38 pKa = 3.61 YY39 pKa = 8.86 TGEE42 pKa = 4.44 GIRR45 pKa = 11.84 IAVIDD50 pKa = 4.09 DD51 pKa = 3.66 GVRR54 pKa = 11.84 YY55 pKa = 6.8 THH57 pKa = 7.46 PDD59 pKa = 2.84 LSGTYY64 pKa = 9.86 DD65 pKa = 3.55 AEE67 pKa = 4.16 NDD69 pKa = 3.66 HH70 pKa = 6.98 NIVTGEE76 pKa = 3.89 GDD78 pKa = 3.45 ATPIADD84 pKa = 4.27 AYY86 pKa = 10.83 SHH88 pKa = 6.21 GTQVSGFSAAAANGTGLVGVAYY110 pKa = 9.83 GATFTGIRR118 pKa = 11.84 ISDD121 pKa = 3.65 ADD123 pKa = 3.95 GEE125 pKa = 4.66 LVNAAPAFHH134 pKa = 6.95 LASDD138 pKa = 4.02 ADD140 pKa = 3.95 VTNNSYY146 pKa = 11.35 GNEE149 pKa = 3.73 IFYY152 pKa = 10.54 EE153 pKa = 4.41 EE154 pKa = 5.16 PGSLDD159 pKa = 4.27 AIADD163 pKa = 3.81 TATTGRR169 pKa = 11.84 AGLGTVHH176 pKa = 6.28 VFASGNSRR184 pKa = 11.84 IQGTLSTHH192 pKa = 6.04 GPEE195 pKa = 4.27 QNSPYY200 pKa = 10.03 QITVAATDD208 pKa = 3.56 ADD210 pKa = 4.02 GQIATFSSPGANVLVAAPGAGVLGVSGEE238 pKa = 4.04 DD239 pKa = 3.74 DD240 pKa = 5.15 GYY242 pKa = 11.48 DD243 pKa = 3.56 LTDD246 pKa = 3.57 GTSFSAPIVSGVAALVLQANPTLGMRR272 pKa = 11.84 DD273 pKa = 3.41 VQEE276 pKa = 3.83 ILAYY280 pKa = 9.9 SAYY283 pKa = 9.48 DD284 pKa = 3.4 TGADD288 pKa = 3.33 PLTRR292 pKa = 11.84 AEE294 pKa = 4.25 ILADD298 pKa = 3.77 TVGSEE303 pKa = 3.92 AALAEE308 pKa = 4.07 LDD310 pKa = 3.9 EE311 pKa = 4.73 PVRR314 pKa = 11.84 AAYY317 pKa = 8.53 EE318 pKa = 4.02 AGISLALSYY327 pKa = 10.5 PWTTVEE333 pKa = 5.66 NGATDD338 pKa = 3.44 WNGGGLTASHH348 pKa = 7.86 DD349 pKa = 3.87 YY350 pKa = 11.59 GFGQVDD356 pKa = 3.16 ARR358 pKa = 11.84 AATRR362 pKa = 11.84 LAEE365 pKa = 4.29 TWDD368 pKa = 3.48 ATPNTVTNQAVVTVTSTTAVAVPDD392 pKa = 3.67 GDD394 pKa = 3.81 AAGVSQSLTVDD405 pKa = 2.67 AAMDD409 pKa = 3.73 VEE411 pKa = 4.61 FATVDD416 pKa = 3.11 VDD418 pKa = 4.43 IPHH421 pKa = 7.39 GDD423 pKa = 3.35 LGQLRR428 pKa = 11.84 IEE430 pKa = 4.31 LTSPHH435 pKa = 6.2 GTTSYY440 pKa = 11.13 LIYY443 pKa = 11.04 NPDD446 pKa = 3.26 FEE448 pKa = 6.43 AYY450 pKa = 9.44 EE451 pKa = 3.73 ILARR455 pKa = 11.84 QDD457 pKa = 3.25 GLLDD461 pKa = 3.98 EE462 pKa = 5.93 LPDD465 pKa = 3.62 LTFFGANVTTLMSSQHH481 pKa = 5.86 YY482 pKa = 9.81 GEE484 pKa = 4.76 SAAGDD489 pKa = 3.21 WTLRR493 pKa = 11.84 VTDD496 pKa = 4.27 TEE498 pKa = 4.42 TGTVGTLEE506 pKa = 4.21 SWSLTLYY513 pKa = 11.17 GDD515 pKa = 3.42 ATTADD520 pKa = 3.57 DD521 pKa = 4.28 RR522 pKa = 11.84 YY523 pKa = 11.2 VYY525 pKa = 9.83 TDD527 pKa = 3.01 QYY529 pKa = 10.65 ATLATADD536 pKa = 3.54 AGRR539 pKa = 11.84 SVLVDD544 pKa = 3.36 SDD546 pKa = 4.01 GGTDD550 pKa = 3.99 TINAAAVSGPVAIHH564 pKa = 7.0 LDD566 pKa = 3.7 GTVGAIYY573 pKa = 10.1 GAAFRR578 pKa = 11.84 VADD581 pKa = 3.65 GTEE584 pKa = 3.65 IEE586 pKa = 4.3 NLYY589 pKa = 10.07 TGDD592 pKa = 4.82 GDD594 pKa = 4.97 DD595 pKa = 4.85 LLAGSALANVLVAGRR610 pKa = 11.84 GSDD613 pKa = 3.45 SLTGGAGDD621 pKa = 5.55 DD622 pKa = 3.95 ILDD625 pKa = 4.28 GGSGLDD631 pKa = 3.26 TARR634 pKa = 11.84 FSGLSSSYY642 pKa = 11.02 AIGVSDD648 pKa = 4.58 SEE650 pKa = 4.79 TVVDD654 pKa = 4.45 GADD657 pKa = 3.38 GRR659 pKa = 11.84 DD660 pKa = 3.01 ILRR663 pKa = 11.84 NTEE666 pKa = 3.58 ILAFDD671 pKa = 4.29 DD672 pKa = 4.4 GNRR675 pKa = 11.84 LSTAPVSVSEE685 pKa = 4.5 IGFDD689 pKa = 3.58 ADD691 pKa = 5.53 FYY693 pKa = 11.53 LATNDD698 pKa = 3.76 DD699 pKa = 3.66 VVAAGFDD706 pKa = 3.34 SGNAYY711 pKa = 10.43 LHH713 pKa = 5.77 YY714 pKa = 10.23 LQFGGFEE721 pKa = 3.98 DD722 pKa = 4.09 RR723 pKa = 11.84 APNALFDD730 pKa = 4.14 SGDD733 pKa = 3.82 YY734 pKa = 10.82 LAVNADD740 pKa = 3.27 VAAAGMNPLLHH751 pKa = 5.53 YY752 pKa = 10.16 HH753 pKa = 7.19 LYY755 pKa = 10.39 GHH757 pKa = 7.37 LEE759 pKa = 3.86 GRR761 pKa = 11.84 DD762 pKa = 3.09 AGAQFDD768 pKa = 4.34 GEE770 pKa = 4.78 AYY772 pKa = 10.47 LAANPDD778 pKa = 3.38 VAGAGIDD785 pKa = 3.73 PMLHH789 pKa = 5.8 YY790 pKa = 9.78 LTVGFGEE797 pKa = 4.4 GRR799 pKa = 11.84 LFQSDD804 pKa = 4.05 PLFGG808 pKa = 4.84
Molecular weight: 83.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.709
IPC2_protein 3.694
IPC_protein 3.757
Toseland 3.516
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.706
Rodwell 3.567
Grimsley 3.414
Solomon 3.757
Lehninger 3.706
Nozaki 3.859
DTASelect 4.151
Thurlkill 3.567
EMBOSS 3.706
Sillero 3.872
Patrickios 0.731
IPC_peptide 3.745
IPC2_peptide 3.846
IPC2.peptide.svr19 3.785
Protein with the highest isoelectric point:
>tr|A0A1G7KP24|A0A1G7KP24_9PROT Acetyl-CoA acetyltransferase OS=Thalassobaculum litoreum DSM 18839 OX=1123362 GN=SAMN05660686_01124 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.1 QPSVLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.63 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 NVIRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.76 GRR39 pKa = 11.84 KK40 pKa = 8.91 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4959
0
4959
1586320
39
2184
319.9
34.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.571 ± 0.054
0.851 ± 0.01
6.302 ± 0.035
5.894 ± 0.032
3.485 ± 0.023
8.907 ± 0.038
2.017 ± 0.02
4.939 ± 0.026
2.834 ± 0.031
9.932 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.529 ± 0.017
2.357 ± 0.02
5.233 ± 0.027
2.822 ± 0.02
7.279 ± 0.036
5.189 ± 0.025
5.56 ± 0.027
7.758 ± 0.028
1.364 ± 0.014
2.176 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here