Cherry mottle leaf virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Trivirinae; Trichovirus

Average proteome isoelectric point is 7.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9IMS9|Q9IMS9_9VIRU 216kDa protein OS=Cherry mottle leaf virus OX=131226 GN=orf1 PE=4 SV=1
MM1 pKa = 7.05TMMMRR6 pKa = 11.84AHH8 pKa = 7.05KK9 pKa = 10.56SKK11 pKa = 10.24IAEE14 pKa = 3.9GDD16 pKa = 3.52IPISGVKK23 pKa = 9.69SSKK26 pKa = 10.3VYY28 pKa = 11.03SDD30 pKa = 3.11VTPFRR35 pKa = 11.84RR36 pKa = 11.84ASDD39 pKa = 5.08LMIHH43 pKa = 5.83WNEE46 pKa = 3.74FAFKK50 pKa = 10.47IMPDD54 pKa = 4.03DD55 pKa = 4.03IGDD58 pKa = 3.66GGFRR62 pKa = 11.84LMSVPVMPKK71 pKa = 10.51DD72 pKa = 3.63EE73 pKa = 4.46IEE75 pKa = 3.87HH76 pKa = 5.63FRR78 pKa = 11.84RR79 pKa = 11.84EE80 pKa = 4.06RR81 pKa = 11.84GSANYY86 pKa = 7.69VHH88 pKa = 7.23WGALSISIDD97 pKa = 3.52ALFKK101 pKa = 10.45KK102 pKa = 10.54QSGVTGRR109 pKa = 11.84CVVFDD114 pKa = 4.97KK115 pKa = 10.75RR116 pKa = 11.84WEE118 pKa = 4.02NCKK121 pKa = 10.32QSILQTFEE129 pKa = 4.59FNLDD133 pKa = 3.41SGSATMITSPNFSVSLDD150 pKa = 3.71DD151 pKa = 5.3PNLNDD156 pKa = 3.72SLCVVVVFNDD166 pKa = 3.2LNFRR170 pKa = 11.84SEE172 pKa = 4.41SYY174 pKa = 9.79PVSVRR179 pKa = 11.84VGNMCRR185 pKa = 11.84FFDD188 pKa = 3.83SFLGSEE194 pKa = 3.97IFKK197 pKa = 10.91NEE199 pKa = 3.87SNVYY203 pKa = 9.36IDD205 pKa = 4.82SSGADD210 pKa = 3.36LLSYY214 pKa = 10.81KK215 pKa = 10.71SFGFGDD221 pKa = 3.66EE222 pKa = 4.48EE223 pKa = 4.02EE224 pKa = 4.43VEE226 pKa = 4.11RR227 pKa = 11.84LFRR230 pKa = 11.84FAEE233 pKa = 4.29VVPTEE238 pKa = 5.0AIDD241 pKa = 3.55LTVRR245 pKa = 11.84EE246 pKa = 4.4IPGSISNLFRR256 pKa = 11.84KK257 pKa = 9.13KK258 pKa = 10.28RR259 pKa = 11.84VMQYY263 pKa = 11.03GYY265 pKa = 10.81GSKK268 pKa = 10.28SDD270 pKa = 3.43PRR272 pKa = 11.84RR273 pKa = 11.84SKK275 pKa = 10.47GKK277 pKa = 9.61RR278 pKa = 11.84LAGVKK283 pKa = 10.03SLALEE288 pKa = 4.79GEE290 pKa = 4.41LNTYY294 pKa = 9.72LNKK297 pKa = 10.6AGEE300 pKa = 4.43SSNTRR305 pKa = 11.84GVEE308 pKa = 3.91ATHH311 pKa = 6.44SKK313 pKa = 7.4VHH315 pKa = 6.47RR316 pKa = 11.84GKK318 pKa = 10.97GNPFGKK324 pKa = 10.35SKK326 pKa = 10.87DD327 pKa = 3.85SQDD330 pKa = 3.23VGAIEE335 pKa = 5.4SNEE338 pKa = 3.6QDD340 pKa = 3.61TYY342 pKa = 11.47RR343 pKa = 11.84VDD345 pKa = 3.67CLSISSEE352 pKa = 3.81SSTPSKK358 pKa = 10.27IRR360 pKa = 11.84AGKK363 pKa = 9.87EE364 pKa = 3.25VDD366 pKa = 3.4SEE368 pKa = 4.9VNFRR372 pKa = 11.84QHH374 pKa = 6.6CNPRR378 pKa = 11.84DD379 pKa = 3.46VGLNGVYY386 pKa = 10.42FNHH389 pKa = 7.08CSGDD393 pKa = 3.48ASDD396 pKa = 4.41GCGSVSVLQSEE407 pKa = 4.43DD408 pKa = 3.75DD409 pKa = 3.72CWVDD413 pKa = 2.91KK414 pKa = 9.68TFRR417 pKa = 11.84VNKK420 pKa = 10.22RR421 pKa = 11.84GG422 pKa = 3.31

Molecular weight: 47.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9IMS7|Q9IMS7_9VIRU Coat protein OS=Cherry mottle leaf virus OX=131226 GN=orf3 PE=4 SV=1
MM1 pKa = 7.71SFNPKK6 pKa = 9.57NNKK9 pKa = 8.48DD10 pKa = 3.35DD11 pKa = 3.78RR12 pKa = 11.84RR13 pKa = 11.84IFSLCLSMMRR23 pKa = 11.84NGIPNGVLAMINMKK37 pKa = 9.84ARR39 pKa = 11.84CVINSEE45 pKa = 4.08KK46 pKa = 11.05VKK48 pKa = 10.85VNKK51 pKa = 10.45LCGKK55 pKa = 10.06SSLSKK60 pKa = 10.32GRR62 pKa = 11.84RR63 pKa = 11.84AARR66 pKa = 11.84LAICSFCYY74 pKa = 9.31RR75 pKa = 11.84TNCTSGYY82 pKa = 9.91RR83 pKa = 11.84CLKK86 pKa = 9.43SRR88 pKa = 11.84NGINARR94 pKa = 11.84FEE96 pKa = 3.7KK97 pKa = 10.93AEE99 pKa = 3.74WVKK102 pKa = 10.91YY103 pKa = 10.05GKK105 pKa = 10.16SSSLFEE111 pKa = 4.34SEE113 pKa = 4.62TPICAPSLRR122 pKa = 11.84NHH124 pKa = 6.88IEE126 pKa = 3.79DD127 pKa = 2.99EE128 pKa = 4.38MNRR131 pKa = 11.84VRR133 pKa = 11.84HH134 pKa = 5.11SS135 pKa = 3.08

Molecular weight: 15.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2637

135

1887

659.3

75.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.93 ± 0.762

2.427 ± 0.576

5.65 ± 1.022

6.902 ± 0.362

5.688 ± 0.472

6.333 ± 0.609

1.972 ± 0.159

6.03 ± 0.541

7.774 ± 0.72

9.063 ± 1.232

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.427 ± 0.369

5.044 ± 1.332

3.413 ± 0.137

3.034 ± 0.698

6.447 ± 0.725

8.95 ± 1.286

4.361 ± 0.654

5.65 ± 0.928

0.872 ± 0.189

3.034 ± 0.347
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski