Ketobacter sp.

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Alcanivoracaceae; Ketobacter; unclassified Ketobacter

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4352 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3F2V250|A0A3F2V250_9GAMM SDR family oxidoreductase OS=Ketobacter sp. OX=2083498 GN=D6160_15460 PE=4 SV=1
MM1 pKa = 7.27KK2 pKa = 7.79TTLAYY7 pKa = 11.25SMIAAMLIPAASMAQDD23 pKa = 3.13RR24 pKa = 11.84KK25 pKa = 10.6EE26 pKa = 4.01SGVYY30 pKa = 9.72IGGAYY35 pKa = 10.21GMYY38 pKa = 9.99KK39 pKa = 10.12VEE41 pKa = 4.51GEE43 pKa = 4.35GKK45 pKa = 10.05NEE47 pKa = 3.6GDD49 pKa = 4.54AKK51 pKa = 10.85FDD53 pKa = 4.15DD54 pKa = 4.76DD55 pKa = 4.31QDD57 pKa = 3.74VFQAILGGQVNKK69 pKa = 10.36YY70 pKa = 9.17FAVEE74 pKa = 4.0GSYY77 pKa = 11.04INFGEE82 pKa = 4.16YY83 pKa = 10.51GGNLASADD91 pKa = 3.66VDD93 pKa = 3.77GMTLAVKK100 pKa = 10.48GILPISDD107 pKa = 3.49VFSIYY112 pKa = 11.05AKK114 pKa = 10.51GGQLWWDD121 pKa = 3.03ADD123 pKa = 3.91YY124 pKa = 11.3QVGEE128 pKa = 4.6GLLEE132 pKa = 4.61ANGSTDD138 pKa = 2.76GSEE141 pKa = 3.88PFYY144 pKa = 11.09GIGVAFAVTDD154 pKa = 3.62RR155 pKa = 11.84MDD157 pKa = 3.24INLDD161 pKa = 3.21YY162 pKa = 10.78TRR164 pKa = 11.84YY165 pKa = 9.91EE166 pKa = 4.03IEE168 pKa = 4.58LEE170 pKa = 3.96TDD172 pKa = 3.32EE173 pKa = 5.14VGLLASDD180 pKa = 4.99SYY182 pKa = 12.07DD183 pKa = 3.38SDD185 pKa = 4.82LDD187 pKa = 3.66QASVGVRR194 pKa = 11.84YY195 pKa = 9.98LFF197 pKa = 4.16

Molecular weight: 21.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3F2V125|A0A3F2V125_9GAMM ATP synthase gamma chain OS=Ketobacter sp. OX=2083498 GN=atpG PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.16RR12 pKa = 11.84KK13 pKa = 9.08RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.22SGRR28 pKa = 11.84QILARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.95GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4352

0

4352

1530567

23

5486

351.7

39.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.891 ± 0.04

1.036 ± 0.012

5.791 ± 0.036

6.068 ± 0.032

4.014 ± 0.023

7.156 ± 0.036

2.237 ± 0.018

5.828 ± 0.027

4.473 ± 0.032

10.524 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.538 ± 0.018

3.93 ± 0.024

4.381 ± 0.027

4.604 ± 0.03

5.415 ± 0.029

6.597 ± 0.03

5.214 ± 0.026

7.057 ± 0.032

1.323 ± 0.014

2.92 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski