Feline stool-associated circular virus KU14

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; Circovirus; unclassified Circovirus

Average proteome isoelectric point is 7.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z6GFU3|A0A2Z6GFU3_9CIRC ATP-dependent helicase Rep OS=Feline stool-associated circular virus KU14 OX=2249941 GN=putative Rep PE=3 SV=1
MM1 pKa = 7.79AATNWCGTLHH11 pKa = 7.04LGDD14 pKa = 3.84EE15 pKa = 4.42EE16 pKa = 5.52FYY18 pKa = 11.49GEE20 pKa = 3.95SWLNQLLGAGKK31 pKa = 8.03ITYY34 pKa = 10.12GIGQIEE40 pKa = 4.69SCPEE44 pKa = 3.26TGRR47 pKa = 11.84RR48 pKa = 11.84HH49 pKa = 4.48FQFYY53 pKa = 9.04VQVGRR58 pKa = 11.84TQKK61 pKa = 9.56LAWMRR66 pKa = 11.84RR67 pKa = 11.84HH68 pKa = 6.4ISDD71 pKa = 3.66KK72 pKa = 11.28AHH74 pKa = 6.49FEE76 pKa = 3.9QARR79 pKa = 11.84GNVQQNIDD87 pKa = 3.76YY88 pKa = 10.56CSKK91 pKa = 10.43QDD93 pKa = 4.01SRR95 pKa = 11.84LDD97 pKa = 3.82GPWEE101 pKa = 3.72VGIARR106 pKa = 11.84RR107 pKa = 11.84QGQTTGLTAATEE119 pKa = 4.44MIASGAAVCEE129 pKa = 4.22VAKK132 pKa = 10.28LYY134 pKa = 9.99PVVWVRR140 pKa = 11.84HH141 pKa = 4.7GKK143 pKa = 10.29GLVHH147 pKa = 7.16LRR149 pKa = 11.84QTLALDD155 pKa = 3.65ADD157 pKa = 3.92RR158 pKa = 11.84RR159 pKa = 11.84EE160 pKa = 4.27FGPDD164 pKa = 3.55GPEE167 pKa = 3.42LWVLYY172 pKa = 10.28GPSGGGKK179 pKa = 9.15SRR181 pKa = 11.84FAKK184 pKa = 9.01EE185 pKa = 3.71HH186 pKa = 5.99WPDD189 pKa = 3.79AFWKK193 pKa = 10.53APYY196 pKa = 8.82SQWWDD201 pKa = 3.38GYY203 pKa = 9.87GKK205 pKa = 9.94HH206 pKa = 5.36EE207 pKa = 4.42TVILDD212 pKa = 3.72DD213 pKa = 4.85FKK215 pKa = 11.59DD216 pKa = 3.68DD217 pKa = 4.08SMRR220 pKa = 11.84LADD223 pKa = 4.51LQRR226 pKa = 11.84LIDD229 pKa = 4.69WYY231 pKa = 9.71PLWVEE236 pKa = 4.28VKK238 pKa = 10.55GGSLPMLATRR248 pKa = 11.84YY249 pKa = 9.99VITSNTHH256 pKa = 5.59PEE258 pKa = 3.69TWYY261 pKa = 9.6TRR263 pKa = 11.84ADD265 pKa = 3.26VHH267 pKa = 4.95KK268 pKa = 8.49TIWRR272 pKa = 11.84RR273 pKa = 11.84VQDD276 pKa = 3.52FAEE279 pKa = 3.95KK280 pKa = 10.14HH281 pKa = 5.05GRR283 pKa = 11.84LLRR286 pKa = 11.84FPPGEE291 pKa = 3.98AAAEE295 pKa = 3.91EE296 pKa = 4.47AAAEE300 pKa = 4.43PGSADD305 pKa = 3.38YY306 pKa = 11.38LGFPDD311 pKa = 5.91VFNSWNPLL319 pKa = 3.29

Molecular weight: 36.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z6GFU3|A0A2Z6GFU3_9CIRC ATP-dependent helicase Rep OS=Feline stool-associated circular virus KU14 OX=2249941 GN=putative Rep PE=3 SV=1
MM1 pKa = 7.26GMGRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84YY8 pKa = 8.49YY9 pKa = 9.64RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84YY13 pKa = 8.81VSRR16 pKa = 11.84PPRR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84MTYY24 pKa = 8.09RR25 pKa = 11.84RR26 pKa = 11.84YY27 pKa = 9.51SRR29 pKa = 11.84RR30 pKa = 11.84YY31 pKa = 8.0CRR33 pKa = 11.84TRR35 pKa = 11.84RR36 pKa = 11.84APKK39 pKa = 8.26GTYY42 pKa = 9.85SSVVTLTQDD51 pKa = 4.15AEE53 pKa = 4.49WKK55 pKa = 10.34FSNQNASNRR64 pKa = 11.84WVAFTFSPVSVPGFLDD80 pKa = 3.47YY81 pKa = 11.04KK82 pKa = 9.87ATYY85 pKa = 9.33SHH87 pKa = 7.01FRR89 pKa = 11.84ILKK92 pKa = 8.91AKK94 pKa = 10.21LYY96 pKa = 10.53LSRR99 pKa = 11.84ALGNDD104 pKa = 2.72AGTTFNYY111 pKa = 10.19LVVGSRR117 pKa = 11.84PFAATQNAGTTTGQDD132 pKa = 3.26ALVPAQTEE140 pKa = 3.94EE141 pKa = 4.27ALRR144 pKa = 11.84QAKK147 pKa = 6.6WQRR150 pKa = 11.84VRR152 pKa = 11.84YY153 pKa = 8.74PSTTTNVVPVGFHH166 pKa = 7.09PYY168 pKa = 10.48AIVQTFGPTPYY179 pKa = 8.24TTGSDD184 pKa = 3.71MMWQRR189 pKa = 11.84IWEE192 pKa = 4.25GRR194 pKa = 11.84RR195 pKa = 11.84WMPFSWAAATGSALSFFGPYY215 pKa = 10.25LVVDD219 pKa = 4.29KK220 pKa = 11.65SNGEE224 pKa = 3.96LSQGSWSVQCTLKK237 pKa = 10.65ISVQFKK243 pKa = 8.92GQRR246 pKa = 3.15

Molecular weight: 28.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

565

246

319

282.5

32.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.673 ± 0.358

1.062 ± 0.164

4.602 ± 1.429

4.425 ± 1.581

4.425 ± 0.568

8.673 ± 0.896

2.301 ± 0.983

2.655 ± 0.68

4.071 ± 0.272

6.903 ± 1.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.947 ± 0.325

2.655 ± 0.395

5.31 ± 0.252

4.956 ± 0.217

8.85 ± 1.673

6.195 ± 1.547

7.08 ± 1.5

6.372 ± 0.625

4.071 ± 0.541

4.779 ± 0.871
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski