Vagococcus zengguangii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Vagococcus

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1965 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D7CUL9|A0A4D7CUL9_9ENTE Helix-turn-helix transcriptional regulator OS=Vagococcus zengguangii OX=2571750 GN=FA707_03375 PE=4 SV=1
MM1 pKa = 7.45TYY3 pKa = 10.72EE4 pKa = 4.13NFAFVYY10 pKa = 10.43DD11 pKa = 3.98EE12 pKa = 4.95VMDD15 pKa = 3.71TTLYY19 pKa = 10.92EE20 pKa = 3.89EE21 pKa = 4.41WLDD24 pKa = 3.57FSKK27 pKa = 10.92RR28 pKa = 11.84HH29 pKa = 5.98LNPDD33 pKa = 3.21TKK35 pKa = 10.72EE36 pKa = 4.07VLEE39 pKa = 4.35LACGTGALAVEE50 pKa = 4.91FAKK53 pKa = 10.69AGYY56 pKa = 9.53EE57 pKa = 4.16VTALDD62 pKa = 4.3LSEE65 pKa = 4.25EE66 pKa = 4.14MLMVASARR74 pKa = 11.84VANEE78 pKa = 3.94DD79 pKa = 3.97EE80 pKa = 4.2EE81 pKa = 6.17LDD83 pKa = 3.6IQFVQGDD90 pKa = 3.94MMDD93 pKa = 3.56LSEE96 pKa = 4.51VGTYY100 pKa = 10.55DD101 pKa = 5.8AITCFSDD108 pKa = 3.78SICYY112 pKa = 9.42MPDD115 pKa = 3.01RR116 pKa = 11.84QAVQQVFDD124 pKa = 3.79TVYY127 pKa = 10.92DD128 pKa = 3.76ALEE131 pKa = 4.32EE132 pKa = 4.35GGKK135 pKa = 10.57FIFDD139 pKa = 3.5VHH141 pKa = 7.19SLHH144 pKa = 6.39QVNEE148 pKa = 3.9VFPDD152 pKa = 3.47YY153 pKa = 10.66SYY155 pKa = 11.22HH156 pKa = 5.76YY157 pKa = 8.56QTEE160 pKa = 4.21DD161 pKa = 4.2FAFLWDD167 pKa = 3.84SFPGEE172 pKa = 4.09KK173 pKa = 10.05EE174 pKa = 3.52NSIEE178 pKa = 3.98HH179 pKa = 6.56FLTFFVKK186 pKa = 10.68DD187 pKa = 3.45EE188 pKa = 4.36EE189 pKa = 4.34NFLRR193 pKa = 11.84FDD195 pKa = 3.64EE196 pKa = 4.27LHH198 pKa = 5.79QEE200 pKa = 3.8RR201 pKa = 11.84TYY203 pKa = 11.04SIEE206 pKa = 3.64NYY208 pKa = 8.96YY209 pKa = 10.71TMLEE213 pKa = 4.2NANFTAIEE221 pKa = 4.39TYY223 pKa = 11.34ADD225 pKa = 3.92FEE227 pKa = 5.5DD228 pKa = 6.32DD229 pKa = 3.78IPTDD233 pKa = 3.56EE234 pKa = 4.13SARR237 pKa = 11.84WFFVCTKK244 pKa = 10.61

Molecular weight: 28.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D7CTN1|A0A4D7CTN1_9ENTE DNA repair protein RecO OS=Vagococcus zengguangii OX=2571750 GN=recO PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.14QPSKK9 pKa = 9.19RR10 pKa = 11.84KK11 pKa = 9.13HH12 pKa = 5.47KK13 pKa = 9.91RR14 pKa = 11.84VHH16 pKa = 6.02GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.73GRR39 pKa = 11.84KK40 pKa = 8.76VLSAA44 pKa = 4.05

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1965

0

1965

630790

36

1694

321.0

36.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.049 ± 0.054

0.614 ± 0.015

5.336 ± 0.047

7.586 ± 0.056

4.361 ± 0.042

6.501 ± 0.051

1.875 ± 0.028

7.649 ± 0.049

6.678 ± 0.047

9.882 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.861 ± 0.026

4.853 ± 0.045

3.349 ± 0.027

4.187 ± 0.04

3.651 ± 0.042

5.937 ± 0.047

5.923 ± 0.044

7.073 ± 0.049

0.894 ± 0.017

3.738 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski