Janthinobacterium sp. HH01
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5974 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L9PM13|L9PM13_9BURK Thiazole synthase OS=Janthinobacterium sp. HH01 OX=1198452 GN=thiG PE=3 SV=1
MM1 pKa = 6.34 YY2 pKa = 7.89 TALFTPAANVSGLASVTVTGGSYY25 pKa = 10.71 TDD27 pKa = 3.95 LLGNQGGAAVTPVIAVDD44 pKa = 3.57 TRR46 pKa = 11.84 PPTLAITSDD55 pKa = 3.66 LAAVHH60 pKa = 6.59 IGDD63 pKa = 3.82 TATVSFTFSEE73 pKa = 4.68 LPVGFAAGDD82 pKa = 3.33 ITTTGGALSGFGVTANPLVYY102 pKa = 9.54 TAIFTPAAGVASGAASISVAGGSYY126 pKa = 10.62 FDD128 pKa = 4.4 LAGNSGAAGATPSIAIDD145 pKa = 3.46 TLAPTVTVGGVAFSADD161 pKa = 3.41 TGASASDD168 pKa = 3.52 LVTRR172 pKa = 11.84 SAAQTVSGTLSAALLAGEE190 pKa = 4.49 VVQVSLDD197 pKa = 4.04 HH198 pKa = 5.91 GQSWQNASGGAGSASWSLAGVTLAGSDD225 pKa = 3.61 TLQVRR230 pKa = 11.84 VNDD233 pKa = 3.63 AAGNHH238 pKa = 5.71 GAAFSAAYY246 pKa = 10.36 VLDD249 pKa = 3.63 ATAPTVLVASDD260 pKa = 4.6 LGALNAGQQAHH271 pKa = 5.45 LTFTFSEE278 pKa = 4.7 APSGLTLASLSATGGAVSALAATANPLVYY307 pKa = 9.44 TALFTPAAGQAGVSASVAVNAGGYY331 pKa = 8.27 TDD333 pKa = 4.46 LAGNGGQSGASLPIVINSALPSVLITSSAAALKK366 pKa = 10.66 SGEE369 pKa = 4.11 TALISFTFSTPPTGFTAADD388 pKa = 3.36 IAVSAGTGALSGFAATANPLVYY410 pKa = 9.44 TALFTPAAGQAAASAAVSVAGGSYY434 pKa = 10.67 TDD436 pKa = 3.28 VFGNNGGGGSSPSIAIDD453 pKa = 3.31 TLAPTVSISSSVAGVKK469 pKa = 9.79 IGEE472 pKa = 4.34 TAVITFTFSEE482 pKa = 4.47 QPLGFTAGDD491 pKa = 3.71 VVTTGGALSNLAVSADD507 pKa = 3.66 PLVYY511 pKa = 9.43 TALFTPTAGLQSTAASVSIGAGAFVDD537 pKa = 4.29 LAGNGATAGASPSLAVDD554 pKa = 3.75 TLAPSAVANTVLFSADD570 pKa = 3.44 SGVSATDD577 pKa = 3.36 LVTRR581 pKa = 11.84 TAAQTISGTLATALGAGEE599 pKa = 4.18 VVEE602 pKa = 4.71 VSLNNGASWSAATAGAGNSWTLAGQTLAGSNTLQVRR638 pKa = 11.84 VSDD641 pKa = 3.54 AAGNHH646 pKa = 5.46 GAAFSTAYY654 pKa = 10.83 VLDD657 pKa = 3.76 NTAPTAAISSSAAALKK673 pKa = 10.67 AGEE676 pKa = 4.26 TATLTFTFSEE686 pKa = 4.68 APSGLGAGSVTVSGGVLGAFTATANPLVYY715 pKa = 9.36 TALFTPTAGVDD726 pKa = 3.3 GGSAGIALNAGGYY739 pKa = 10.0 SDD741 pKa = 3.7 TAGNSGGGYY750 pKa = 6.57 TLPAIAVDD758 pKa = 3.9 TQAPSIAGASVALSADD774 pKa = 3.51 SGVAADD780 pKa = 5.73 LITNIAAQTLSGTLNGNLSAGDD802 pKa = 3.8 TVEE805 pKa = 4.28 VSLDD809 pKa = 3.49 NGASWSAAAGAVGTAAWTLAGQTLTAGAGHH839 pKa = 5.41 QVQVRR844 pKa = 11.84 VSDD847 pKa = 3.39 AAGNHH852 pKa = 5.76 GAVASSAYY860 pKa = 10.69 VLDD863 pKa = 3.93 QSAPTLQISSSKK875 pKa = 9.55 TVLNSAEE882 pKa = 4.28 TATLTFTFSEE892 pKa = 4.73 APQGFTAGAIAATGGAVSGFAATGGAVSGFAATANPLVYY931 pKa = 9.46 TAVFTPTAGQAAGVGAVSVAAGAYY955 pKa = 8.26 TDD957 pKa = 3.74 AAGNSGLAGAGPAISYY973 pKa = 7.82 ATVAPGVAISSDD985 pKa = 3.55 VSALHH990 pKa = 6.86 AGQTAIISFKK1000 pKa = 10.78 FSVPPLGFTASDD1012 pKa = 3.33 VSTSNGTLGALTATADD1028 pKa = 3.82 PLLYY1032 pKa = 9.95 TAVFTPAANLAGGGGGVSIGAGLFVDD1058 pKa = 4.76 GLGNAGSAAAMAPISIDD1075 pKa = 3.18 TLAPTLAITSGAAALNSGATSLITFTFSEE1104 pKa = 4.65 APPAFSLGDD1113 pKa = 3.18 ITVANGTLANLVQSANPLVYY1133 pKa = 9.48 TAVFTPGANIAAGAATIAVIGGAYY1157 pKa = 8.73 TDD1159 pKa = 3.86 LAGNGGGAAPTPSISIDD1176 pKa = 3.27 TLAPTIAGASLLFSADD1192 pKa = 3.35 TGSSNTDD1199 pKa = 4.19 LITRR1203 pKa = 11.84 TAAQTIAGTVNGLLAAGDD1221 pKa = 3.9 VVEE1224 pKa = 4.67 VSFDD1228 pKa = 3.63 SGASWVGASAAAGGSSWVLAGQTLGGSGTLQVRR1261 pKa = 11.84 VSDD1264 pKa = 3.86 ANGNHH1269 pKa = 5.87 GAVYY1273 pKa = 10.46 ARR1275 pKa = 11.84 AYY1277 pKa = 10.79 VLDD1280 pKa = 3.87 TTAPTVAISSDD1291 pKa = 3.2 TATLRR1296 pKa = 11.84 NGQAATISFTFSEE1309 pKa = 4.83 APAGFTLGDD1318 pKa = 4.54 LIATGGTLSGFAATANPLVYY1338 pKa = 9.71 TVLLTPTPGLAGAAGVTLGNGLYY1361 pKa = 10.54 ADD1363 pKa = 3.98 AAGNSGSGAGSPAISVDD1380 pKa = 3.99 TIAPTLAIAASSAALKK1396 pKa = 10.93 AGDD1399 pKa = 3.58 TAVITFTFSDD1409 pKa = 3.83 APAAFTLADD1418 pKa = 3.48 VSASHH1423 pKa = 6.21 GTLSALAAGPDD1434 pKa = 3.58 PRR1436 pKa = 11.84 VYY1438 pKa = 10.06 TAVFTPDD1445 pKa = 3.25 AGTAAASAVVSVAGSVYY1462 pKa = 11.04 ADD1464 pKa = 3.18 AAGNTGSSVSSGAIAIDD1481 pKa = 3.44 TLAPTTSGATVVFSADD1497 pKa = 3.4 YY1498 pKa = 9.98 GASGSDD1504 pKa = 3.19 LVTNAVGQVISGTLDD1519 pKa = 3.07 APLAAGEE1526 pKa = 4.07 QVQVSLDD1533 pKa = 3.73 NGASWLNAVAAAGLSSWALAPQLLNGSGTLQVRR1566 pKa = 11.84 VADD1569 pKa = 3.67 AAGNHH1574 pKa = 5.87 GPAFSAGYY1582 pKa = 10.32 VLDD1585 pKa = 4.33 LVAPAMTVTSSAAALKK1601 pKa = 10.71 AGDD1604 pKa = 4.03 SATLTFTFSEE1614 pKa = 4.7 APTGFTQADD1623 pKa = 3.93 LVALNGTLSGFTPTANPLVYY1643 pKa = 9.83 TVVLTPAAGLGGVSAGVTLAAGLYY1667 pKa = 9.4 TDD1669 pKa = 3.91 VAGNPGAAAASPLVAIDD1686 pKa = 3.7 TQPPTVAITTSATQLKK1702 pKa = 9.63 IGEE1705 pKa = 4.52 LATITFTFNEE1715 pKa = 4.26 APLGFTAGDD1724 pKa = 3.64 VAVTGGSLSGFGLTANPLVYY1744 pKa = 8.02 TTLFTPTPGVAGGTASIAIGAGDD1767 pKa = 4.0 YY1768 pKa = 11.12 LDD1770 pKa = 4.93 AAGNPGLAAAAPAIQFSTLAPATGGASVSFSADD1803 pKa = 2.37 TGVFNNDD1810 pKa = 3.31 LVTSSALQTINGTLDD1825 pKa = 3.0 ANLAVGEE1832 pKa = 4.1 AVEE1835 pKa = 4.3 VSLDD1839 pKa = 3.66 NGASWSTALAATGSPAWSLGPLVLGGSGVLQVRR1872 pKa = 11.84 VTDD1875 pKa = 3.55 AAGNHH1880 pKa = 5.75 GAASASAYY1888 pKa = 10.75 VLDD1891 pKa = 4.05 TGAPGVAISADD1902 pKa = 3.44 AAALKK1907 pKa = 10.76 AGDD1910 pKa = 3.99 SATLSFTFTEE1920 pKa = 4.8 APHH1923 pKa = 6.7 GFTTADD1929 pKa = 3.28 VVAVGGIKK1937 pKa = 10.41 GALTATANPLVYY1949 pKa = 10.09 TMSYY1953 pKa = 9.3 TPTAGFSGVGGVTLTAASYY1972 pKa = 10.77 TDD1974 pKa = 3.92 LAGNPGAGAAGPALSVDD1991 pKa = 3.78 AQPPTLSITSSSTALKK2007 pKa = 10.68 AGDD2010 pKa = 3.87 SATLTFTFSEE2020 pKa = 4.63 APLGFAAGDD2029 pKa = 3.51 ITVSGGTIGGFSATANPLVYY2049 pKa = 9.46 TAVFTPTPGTAAGSAVIAVASGVYY2073 pKa = 10.18 SDD2075 pKa = 4.12 AAGNGGASGSAPAIVIDD2092 pKa = 3.96 TLAPLAAVGGAIAFSTDD2109 pKa = 2.86 TGISNNDD2116 pKa = 4.09 LITSVAAQTVSGTLSAPLAAGEE2138 pKa = 4.26 TVQVSFDD2145 pKa = 4.38 NGASWQAAAVSGTGWSAGVTLTGSGVMRR2173 pKa = 11.84 LYY2175 pKa = 11.23 VSDD2178 pKa = 3.56 AAGNHH2183 pKa = 5.49 SPEE2186 pKa = 3.85 IVQAYY2191 pKa = 9.73 QYY2193 pKa = 10.87 DD2194 pKa = 3.91 ATPPTVAITASAAVANGMAPTTITFSFSEE2223 pKa = 4.42 APQGFTAADD2232 pKa = 3.71 LTVSGGTLGAVTATANPLVYY2252 pKa = 9.61 TVQFTPTPGQASGIATITLTGGGYY2276 pKa = 10.14 VDD2278 pKa = 3.89 AAGNNGAGNALPALSFDD2295 pKa = 3.97 TMPPAAAAAGAQFSSDD2311 pKa = 3.19 SGTPGDD2317 pKa = 4.65 MITNNPSQTLTGTLSAPLAAGDD2339 pKa = 3.99 VVQVSLDD2346 pKa = 4.13 DD2347 pKa = 4.06 GVNWNIATVSGTTWTLAGRR2366 pKa = 11.84 TLQGSSRR2373 pKa = 11.84 MVVRR2377 pKa = 11.84 VSDD2380 pKa = 3.49 AAGNVSTPMVMPYY2393 pKa = 10.69 VLDD2396 pKa = 3.79 TQAPTVTITSSVTQLGEE2413 pKa = 4.14 GGSAQITLTLSDD2425 pKa = 4.4 PGVLTLADD2433 pKa = 3.41 IGVSGGTLSNLSGGGTVYY2451 pKa = 10.62 TVMLTPPPFSVTPITVSVAGHH2472 pKa = 5.74 VFSDD2476 pKa = 3.48 AAGNASAAAAPLQLGVNTFPTPISGTPSTVDD2507 pKa = 3.06 GVQIMTQTGIDD2518 pKa = 3.48 ARR2520 pKa = 11.84 TGVATRR2526 pKa = 11.84 TVTVPIISDD2535 pKa = 3.51 TRR2537 pKa = 11.84 SEE2539 pKa = 4.18 DD2540 pKa = 2.99 HH2541 pKa = 6.3 STPHH2545 pKa = 6.88 ANLADD2550 pKa = 3.68 IPLGLAAAGATPGTSLVVSLPVGVGLEE2577 pKa = 3.94 AAGPSVLLGGATALTDD2593 pKa = 4.62 LIGRR2597 pKa = 11.84 IDD2599 pKa = 3.78 DD2600 pKa = 3.84 HH2601 pKa = 6.14 TLQGQATRR2609 pKa = 11.84 AAMEE2613 pKa = 4.35 AQASAYY2619 pKa = 9.67 LASLAPGVQLQQGTLTLGAASAVADD2644 pKa = 4.08 GAEE2647 pKa = 4.0 IMITGAGATAGGAPHH2662 pKa = 6.96 GALYY2666 pKa = 8.43 DD2667 pKa = 3.77 TATGGTAVALVIDD2680 pKa = 4.9 ARR2682 pKa = 11.84 ALPQGIGLEE2691 pKa = 3.96 MAGIDD2696 pKa = 3.41 FAAFIGAATVRR2707 pKa = 11.84 GGAGQNFFIGDD2718 pKa = 3.74 AAAQRR2723 pKa = 11.84 IVLTDD2728 pKa = 3.23 GGGNDD2733 pKa = 3.45 TLYY2736 pKa = 11.32 GNGGNDD2742 pKa = 3.42 VLGTAGGRR2750 pKa = 11.84 DD2751 pKa = 4.02 YY2752 pKa = 11.73 LDD2754 pKa = 3.91 GGAGNDD2760 pKa = 3.48 ILFGGAGNDD2769 pKa = 3.74 VLNGGADD2776 pKa = 3.57 NDD2778 pKa = 4.29 TLQGGRR2784 pKa = 11.84 SDD2786 pKa = 3.36 TGQWQFYY2793 pKa = 9.25 LNPAGAVVGRR2803 pKa = 11.84 HH2804 pKa = 5.04 EE2805 pKa = 4.39 MALTDD2810 pKa = 3.76 AAATEE2815 pKa = 4.61 TVTAAQLNQDD2825 pKa = 3.41 VALLGFAGPKK2835 pKa = 9.37 AANLQDD2841 pKa = 3.3 WSLMYY2846 pKa = 10.23 HH2847 pKa = 6.69 AAYY2850 pKa = 9.91 HH2851 pKa = 6.37 RR2852 pKa = 11.84 APDD2855 pKa = 4.34 LGGLSYY2861 pKa = 10.53 WSTSGGTPQQHH2872 pKa = 6.26 AAGFLQSPEE2881 pKa = 3.88 GLNGIMALNNHH2892 pKa = 6.54 DD2893 pKa = 4.61 FVVQLLQNSVGRR2905 pKa = 11.84 AGTAAEE2911 pKa = 4.26 LSRR2914 pKa = 11.84 WIGMLDD2920 pKa = 3.59 AAPGDD2925 pKa = 3.84 MQVRR2929 pKa = 11.84 GAVFTDD2935 pKa = 3.26 IALSAEE2941 pKa = 3.88 HH2942 pKa = 6.56 RR2943 pKa = 11.84 AGYY2946 pKa = 7.96 ITADD2950 pKa = 3.64 GVPLGGEE2957 pKa = 4.06 LLADD2961 pKa = 3.56 EE2962 pKa = 5.47 RR2963 pKa = 11.84 GWIAGSGDD2971 pKa = 3.65 DD2972 pKa = 4.14 RR2973 pKa = 11.84 LQGGGGNDD2981 pKa = 3.83 LLVGGDD2987 pKa = 3.71 GVDD2990 pKa = 3.28 TVVYY2994 pKa = 10.28 GDD2996 pKa = 3.89 AASSHH3001 pKa = 5.49 SVVLGRR3007 pKa = 11.84 TGEE3010 pKa = 4.14 VMIAAPDD3017 pKa = 3.66 GGRR3020 pKa = 11.84 DD3021 pKa = 3.33 TLRR3024 pKa = 11.84 QIEE3027 pKa = 4.11 RR3028 pKa = 11.84 GEE3030 pKa = 4.1 FKK3032 pKa = 10.87 GGEE3035 pKa = 4.24 TVDD3038 pKa = 4.69 LGFTQASAKK3047 pKa = 10.19 ALQEE3051 pKa = 3.95 LGMMYY3056 pKa = 10.53 HH3057 pKa = 6.34 LTLNRR3062 pKa = 11.84 AADD3065 pKa = 3.72 LGGFQFWLASGLEE3078 pKa = 4.38 GSALAHH3084 pKa = 6.55 GFTDD3088 pKa = 3.56 STEE3091 pKa = 3.69 MAQRR3095 pKa = 11.84 FGQLDD3100 pKa = 3.37 DD3101 pKa = 4.29 AGFVKK3106 pKa = 10.77 LLYY3109 pKa = 10.44 QNTVRR3114 pKa = 11.84 HH3115 pKa = 6.41 DD3116 pKa = 3.49 PDD3118 pKa = 3.6 AATLARR3124 pKa = 11.84 WDD3126 pKa = 3.82 SFLDD3130 pKa = 3.36 SHH3132 pKa = 6.94 HH3133 pKa = 6.77 RR3134 pKa = 11.84 ADD3136 pKa = 5.46 LVALLASDD3144 pKa = 3.55 VTLIGSQYY3152 pKa = 9.46 GTDD3155 pKa = 3.32 GMNLIGGLL3163 pKa = 3.73
Molecular weight: 305.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.741
IPC2_protein 3.795
IPC_protein 3.859
Toseland 3.617
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.821
Rodwell 3.681
Grimsley 3.528
Solomon 3.872
Lehninger 3.821
Nozaki 3.973
DTASelect 4.291
Thurlkill 3.681
EMBOSS 3.834
Sillero 3.986
Patrickios 2.117
IPC_peptide 3.859
IPC2_peptide 3.961
IPC2.peptide.svr19 3.878
Protein with the highest isoelectric point:
>tr|L9PC82|L9PC82_9BURK Flagellar hook-length control protein OS=Janthinobacterium sp. HH01 OX=1198452 GN=Jab_2c12740 PE=4 SV=1
MM1 pKa = 7.91 PKK3 pKa = 9.63 MKK5 pKa = 9.83 TKK7 pKa = 10.72 SSAKK11 pKa = 9.15 KK12 pKa = 9.67 RR13 pKa = 11.84 FRR15 pKa = 11.84 VRR17 pKa = 11.84 PGGTVKK23 pKa = 10.54 SGMAFKK29 pKa = 10.69 RR30 pKa = 11.84 HH31 pKa = 5.84 ILTKK35 pKa = 9.78 KK36 pKa = 3.98 TTKK39 pKa = 10.23 NKK41 pKa = 9.21 RR42 pKa = 11.84 QLRR45 pKa = 11.84 GTRR48 pKa = 11.84 NINASDD54 pKa = 3.65 VTSVMRR60 pKa = 11.84 MMPTAA65 pKa = 4.09
Molecular weight: 7.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.358
IPC2_protein 10.789
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.515
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.427
Grimsley 12.544
Solomon 12.983
Lehninger 12.896
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.149
IPC_peptide 12.998
IPC2_peptide 11.974
IPC2.peptide.svr19 9.0
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5974
0
5974
2157853
29
9114
361.2
39.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.79 ± 0.054
0.835 ± 0.01
5.393 ± 0.02
5.012 ± 0.029
3.489 ± 0.021
8.073 ± 0.031
2.166 ± 0.015
4.659 ± 0.022
3.665 ± 0.031
10.684 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.561 ± 0.019
3.123 ± 0.023
4.891 ± 0.025
4.227 ± 0.028
6.158 ± 0.029
5.784 ± 0.025
5.307 ± 0.037
7.207 ± 0.028
1.346 ± 0.012
2.631 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here