Orientia chuto str. Dubai
Average proteome isoelectric point is 7.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1067 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F3MGX4|A0A0F3MGX4_9RICK Uncharacterized protein OS=Orientia chuto str. Dubai OX=1359168 GN=OCHUTO_1021 PE=4 SV=1
MM1 pKa = 7.81 IYY3 pKa = 10.71 FLMHH7 pKa = 5.16 MRR9 pKa = 11.84 MYY11 pKa = 9.95 TIEE14 pKa = 4.18 SSASLEE20 pKa = 4.1 MQQEE24 pKa = 3.69 DD25 pKa = 5.22 DD26 pKa = 3.15 IFYY29 pKa = 10.78 DD30 pKa = 3.54 AFEE33 pKa = 5.79 DD34 pKa = 4.04 IPNEE38 pKa = 4.11 STDD41 pKa = 3.5 VSDD44 pKa = 6.07 AEE46 pKa = 4.36 MKK48 pKa = 10.93 VLGNVDD54 pKa = 4.31 LSCFDD59 pKa = 3.42 TRR61 pKa = 11.84 GVV63 pKa = 3.27
Molecular weight: 7.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.761
IPC2_protein 3.77
IPC_protein 3.694
Toseland 3.49
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.414
Solomon 3.656
Lehninger 3.617
Nozaki 3.834
DTASelect 4.012
Thurlkill 3.567
EMBOSS 3.656
Sillero 3.821
Patrickios 1.863
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.748
Protein with the highest isoelectric point:
>tr|A0A0F3MN45|A0A0F3MN45_9RICK TCP-1/cpn60 chaperonin family protein OS=Orientia chuto str. Dubai OX=1359168 GN=OCHUTO_0639 PE=4 SV=1
MM1 pKa = 7.13 YY2 pKa = 10.08 SSKK5 pKa = 10.5 SGHH8 pKa = 6.58 LEE10 pKa = 3.85 QPEE13 pKa = 4.26 KK14 pKa = 9.99 TWRR17 pKa = 11.84 KK18 pKa = 7.89 VCARR22 pKa = 11.84 RR23 pKa = 11.84 HH24 pKa = 4.24 RR25 pKa = 11.84 HH26 pKa = 5.41 RR27 pKa = 11.84 KK28 pKa = 8.49 FKK30 pKa = 9.44 NTQPRR35 pKa = 11.84 RR36 pKa = 11.84 TFASCMADD44 pKa = 2.97 AGAGQYY50 pKa = 10.36 IIGIVLIIMTQNQQVFMLEE69 pKa = 4.22 LVV71 pKa = 3.47
Molecular weight: 8.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.326
IPC2_protein 9.633
IPC_protein 10.145
Toseland 10.599
ProMoST 10.204
Dawson 10.701
Bjellqvist 10.379
Wikipedia 10.862
Rodwell 11.052
Grimsley 10.745
Solomon 10.774
Lehninger 10.76
Nozaki 10.599
DTASelect 10.365
Thurlkill 10.599
EMBOSS 10.994
Sillero 10.628
Patrickios 10.847
IPC_peptide 10.789
IPC2_peptide 9.414
IPC2.peptide.svr19 8.547
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1067
0
1067
267194
28
1959
250.4
28.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.192 ± 0.071
1.417 ± 0.038
4.594 ± 0.055
5.203 ± 0.072
4.456 ± 0.059
4.988 ± 0.063
2.028 ± 0.033
10.696 ± 0.097
7.84 ± 0.079
10.203 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.146 ± 0.04
6.938 ± 0.076
2.905 ± 0.043
4.085 ± 0.06
3.292 ± 0.048
7.486 ± 0.069
4.994 ± 0.055
5.708 ± 0.061
0.805 ± 0.026
4.022 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here