Burkholderia dabaoshanensis
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6323 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2N7VNZ1|A0A2N7VNZ1_9BURK DNA-deoxyinosine glycosylase OS=Burkholderia dabaoshanensis OX=564714 GN=C0Z18_15985 PE=4 SV=1
MM1 pKa = 7.63 SDD3 pKa = 3.45 VIEE6 pKa = 4.66 FKK8 pKa = 10.75 SWVCVICGWVYY19 pKa = 11.07 NEE21 pKa = 4.58 EE22 pKa = 3.95 EE23 pKa = 4.29 GLPDD27 pKa = 3.52 EE28 pKa = 5.75 GIAPGTRR35 pKa = 11.84 FADD38 pKa = 3.32 IPEE41 pKa = 4.21 DD42 pKa = 3.69 WRR44 pKa = 11.84 CPLCDD49 pKa = 3.39 VSKK52 pKa = 11.62 GEE54 pKa = 3.91 FAAVEE59 pKa = 4.23 FF60 pKa = 4.64
Molecular weight: 6.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.799
IPC2_protein 3.986
IPC_protein 3.834
Toseland 3.668
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.719
Rodwell 3.681
Grimsley 3.592
Solomon 3.783
Lehninger 3.745
Nozaki 3.948
DTASelect 4.062
Thurlkill 3.719
EMBOSS 3.732
Sillero 3.961
Patrickios 1.875
IPC_peptide 3.783
IPC2_peptide 3.935
IPC2.peptide.svr19 3.867
Protein with the highest isoelectric point:
>tr|A0A2N7VSI3|A0A2N7VSI3_9BURK Uncharacterized protein OS=Burkholderia dabaoshanensis OX=564714 GN=C0Z18_11900 PE=4 SV=1
MM1 pKa = 7.62 HH2 pKa = 7.1 KK3 pKa = 10.37 RR4 pKa = 11.84 LTFRR8 pKa = 11.84 HH9 pKa = 5.6 SRR11 pKa = 11.84 PPAALNPARR20 pKa = 11.84 SLRR23 pKa = 11.84 RR24 pKa = 11.84 TPARR28 pKa = 11.84 ALRR31 pKa = 11.84 HH32 pKa = 4.74 HH33 pKa = 7.4 AARR36 pKa = 11.84 AVGLSRR42 pKa = 11.84 RR43 pKa = 11.84 GTAPAHH49 pKa = 4.95 QQQADD54 pKa = 3.52 GSLRR58 pKa = 11.84 AWLHH62 pKa = 5.93 AVFSMLRR69 pKa = 11.84 SRR71 pKa = 11.84 TRR73 pKa = 11.84 ARR75 pKa = 11.84 RR76 pKa = 11.84 VLLRR80 pKa = 11.84 GLLRR84 pKa = 11.84 HH85 pKa = 5.68 TKK87 pKa = 9.96 ARR89 pKa = 11.84 RR90 pKa = 11.84 AALAAAQRR98 pKa = 11.84 AAAVSNRR105 pKa = 11.84 RR106 pKa = 11.84 PRR108 pKa = 11.84 RR109 pKa = 11.84 LSASAGWFAFAARR122 pKa = 4.17
Molecular weight: 13.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.33
IPC_protein 12.939
Toseland 13.1
ProMoST 13.598
Dawson 13.1
Bjellqvist 13.1
Wikipedia 13.583
Rodwell 12.632
Grimsley 13.144
Solomon 13.598
Lehninger 13.495
Nozaki 13.1
DTASelect 13.1
Thurlkill 13.1
EMBOSS 13.598
Sillero 13.1
Patrickios 12.34
IPC_peptide 13.598
IPC2_peptide 12.588
IPC2.peptide.svr19 9.266
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6323
0
6323
2062746
12
4999
326.2
35.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.229 ± 0.048
0.959 ± 0.011
5.406 ± 0.022
5.28 ± 0.027
3.609 ± 0.019
8.135 ± 0.031
2.321 ± 0.014
4.565 ± 0.023
2.915 ± 0.027
10.124 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.309 ± 0.013
2.643 ± 0.021
5.123 ± 0.024
3.502 ± 0.021
7.313 ± 0.038
5.745 ± 0.028
5.361 ± 0.026
7.667 ± 0.026
1.381 ± 0.013
2.413 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here