bacterium HR36

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2862 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H6AEK2|A0A2H6AEK2_9BACT Uncharacterized protein OS=bacterium HR36 OX=2035431 GN=HRbin36_01500 PE=4 SV=1
MM1 pKa = 7.75PSLTVSLPSLTEE13 pKa = 3.76LHH15 pKa = 6.38EE16 pKa = 4.46GEE18 pKa = 4.48YY19 pKa = 10.92VYY21 pKa = 10.68LYY23 pKa = 10.04PDD25 pKa = 3.56VNHH28 pKa = 6.98TPQCSSQPLTFSASGLPDD46 pKa = 4.0GLSLDD51 pKa = 4.04HH52 pKa = 6.44STGLITGVLPHH63 pKa = 6.65SLANCTNPIRR73 pKa = 11.84EE74 pKa = 4.07YY75 pKa = 10.58TITLTATDD83 pKa = 5.42GIDD86 pKa = 3.36TASASATLRR95 pKa = 11.84IHH97 pKa = 5.49NTDD100 pKa = 3.26YY101 pKa = 11.22TLLSPGDD108 pKa = 3.59QSNTEE113 pKa = 3.68GDD115 pKa = 4.12YY116 pKa = 11.35VYY118 pKa = 11.4LDD120 pKa = 4.14LQSLWGSAYY129 pKa = 10.46AGLNMQAPLIFSTPSTSAPNSLPPGLSLDD158 pKa = 3.92PNTGIVSGTISSDD171 pKa = 2.63AATPNNSPYY180 pKa = 10.6SYY182 pKa = 10.73YY183 pKa = 9.8VTITLQNLADD193 pKa = 4.78GDD195 pKa = 3.96TSTISFNWAVIDD207 pKa = 4.02NPNLAEE213 pKa = 4.51PPPDD217 pKa = 4.75PGNDD221 pKa = 3.49PPPEE225 pKa = 4.2NEE227 pKa = 4.11PPTIEE232 pKa = 4.37ALSLVNVSDD241 pKa = 4.11ASRR244 pKa = 11.84NLAPRR249 pKa = 11.84TQDD252 pKa = 3.46LLQATVIGVSDD263 pKa = 4.29PDD265 pKa = 3.8GDD267 pKa = 4.0PVSVRR272 pKa = 11.84LEE274 pKa = 3.7WRR276 pKa = 11.84VNGVTVQTVEE286 pKa = 4.27GTSATFDD293 pKa = 3.73LAQPGHH299 pKa = 6.77GDD301 pKa = 2.95VGDD304 pKa = 3.84QVTVAVILDD313 pKa = 4.32DD314 pKa = 4.11GSGPIEE320 pKa = 3.97AASSNVTVANTPPVVTDD337 pKa = 3.11ILVEE341 pKa = 3.78QGYY344 pKa = 7.9QAFEE348 pKa = 4.64DD349 pKa = 3.67DD350 pKa = 3.54VYY352 pKa = 11.56YY353 pKa = 11.14YY354 pKa = 11.25LVGQRR359 pKa = 11.84AAMHH363 pKa = 6.48FLVQDD368 pKa = 3.66DD369 pKa = 6.13DD370 pKa = 3.88MDD372 pKa = 3.72QGQGNDD378 pKa = 3.4QLRR381 pKa = 11.84LEE383 pKa = 4.74VGGEE387 pKa = 3.93LPPAEE392 pKa = 4.46NGWYY396 pKa = 9.84ISDD399 pKa = 3.77YY400 pKa = 11.02QNGEE404 pKa = 4.01FFLEE408 pKa = 4.0ATIADD413 pKa = 4.57HH414 pKa = 7.33GDD416 pKa = 3.19PDD418 pKa = 4.27QPTKK422 pKa = 10.91YY423 pKa = 10.24YY424 pKa = 9.87PLNICAKK431 pKa = 9.8DD432 pKa = 3.49NYY434 pKa = 10.51DD435 pKa = 4.37AGDD438 pKa = 3.77PPIILAVTHH447 pKa = 5.66PHH449 pKa = 4.24VTRR452 pKa = 11.84ILVTPPATMAGWGTALNPYY471 pKa = 10.02LMRR474 pKa = 11.84AGRR477 pKa = 11.84RR478 pKa = 11.84EE479 pKa = 4.0TFGVRR484 pKa = 11.84IEE486 pKa = 4.2GVGFGGGVPWGGWIWWRR503 pKa = 11.84LWDD506 pKa = 5.61DD507 pKa = 4.59DD508 pKa = 5.05FADD511 pKa = 4.62SLPNQNDD518 pKa = 3.66LLQGDD523 pKa = 3.9TRR525 pKa = 11.84EE526 pKa = 4.16TVTMVGGPTGAWFVNTTFEE545 pKa = 4.24LTADD549 pKa = 3.81AASDD553 pKa = 4.36DD554 pKa = 4.0IIGRR558 pKa = 11.84DD559 pKa = 3.67GNTGAGWPEE568 pKa = 3.9GAVKK572 pKa = 10.18EE573 pKa = 4.15LYY575 pKa = 10.43FYY577 pKa = 9.97VWWWYY582 pKa = 7.79TVLWGSARR590 pKa = 11.84SPIFYY595 pKa = 10.61VEE597 pKa = 4.12WVPP600 pKa = 4.82

Molecular weight: 64.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H6ACE3|A0A2H6ACE3_9BACT Uncharacterized protein OS=bacterium HR36 OX=2035431 GN=HRbin36_00745 PE=4 SV=1
MM1 pKa = 7.42SLAFEE6 pKa = 4.29NQVVLITGAARR17 pKa = 11.84GLGRR21 pKa = 11.84QLALEE26 pKa = 4.28MALHH30 pKa = 6.27GAVIAAIDD38 pKa = 3.84RR39 pKa = 11.84DD40 pKa = 4.25VEE42 pKa = 4.19PLASLMQEE50 pKa = 3.71LKK52 pKa = 10.44EE53 pKa = 4.09RR54 pKa = 11.84GRR56 pKa = 11.84AGAFAQADD64 pKa = 3.74VTQRR68 pKa = 11.84QQLFQAVQSVEE79 pKa = 3.87QHH81 pKa = 6.33LGPVSILIANAGIGSGTSALAFDD104 pKa = 4.84PEE106 pKa = 4.23QFAEE110 pKa = 4.27MVEE113 pKa = 4.25VNLVGVANSIAAVLPGMLQRR133 pKa = 11.84RR134 pKa = 11.84RR135 pKa = 11.84GHH137 pKa = 6.4LVAISSLASLRR148 pKa = 11.84GLAFLGGYY156 pKa = 8.21CASKK160 pKa = 10.85AGVNALMEE168 pKa = 4.46SLRR171 pKa = 11.84LEE173 pKa = 4.06LAPRR177 pKa = 11.84DD178 pKa = 3.64IACTIICPGWIRR190 pKa = 11.84TNLTAHH196 pKa = 7.01LPLPKK201 pKa = 9.33PDD203 pKa = 3.16IMPVEE208 pKa = 4.0EE209 pKa = 3.69AARR212 pKa = 11.84RR213 pKa = 11.84IVRR216 pKa = 11.84AIRR219 pKa = 11.84QRR221 pKa = 11.84RR222 pKa = 11.84AFYY225 pKa = 10.63AFPWSTAWILRR236 pKa = 11.84LCRR239 pKa = 11.84LLPPSLSDD247 pKa = 3.01WLLRR251 pKa = 11.84SYY253 pKa = 11.04GRR255 pKa = 11.84RR256 pKa = 11.84LQKK259 pKa = 10.44KK260 pKa = 8.51LEE262 pKa = 4.12RR263 pKa = 11.84FTRR266 pKa = 11.84RR267 pKa = 3.0

Molecular weight: 29.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2862

0

2862

869442

32

2200

303.8

33.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.132 ± 0.047

1.367 ± 0.019

4.601 ± 0.034

6.235 ± 0.05

3.434 ± 0.025

7.435 ± 0.04

2.536 ± 0.025

4.491 ± 0.032

3.021 ± 0.036

11.348 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.77 ± 0.018

2.57 ± 0.031

6.219 ± 0.044

4.9 ± 0.037

8.253 ± 0.044

5.02 ± 0.036

4.761 ± 0.038

7.319 ± 0.03

1.945 ± 0.026

2.643 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski