Chickpea chlorotic dwarf virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8K7V6|F8K7V6_9GEMI Replication-associated protein OS=Chickpea chlorotic dwarf virus OX=463360 GN=repA PE=3 SV=1
MM1 pKa = 7.73PSANKK6 pKa = 9.92NFRR9 pKa = 11.84FQSKK13 pKa = 8.6YY14 pKa = 10.68VFLTYY19 pKa = 9.4PKK21 pKa = 10.35CSSQRR26 pKa = 11.84DD27 pKa = 3.55ALLEE31 pKa = 4.0FLWEE35 pKa = 4.03KK36 pKa = 9.44LTPFLIYY43 pKa = 10.06FIGVATEE50 pKa = 3.71LHH52 pKa = 5.95QDD54 pKa = 3.29GTTHH58 pKa = 5.38YY59 pKa = 9.89HH60 pKa = 7.28ALIQLDD66 pKa = 4.12KK67 pKa = 10.29RR68 pKa = 11.84PHH70 pKa = 6.03IRR72 pKa = 11.84DD73 pKa = 3.11PSFFDD78 pKa = 4.7FEE80 pKa = 4.9GNHH83 pKa = 6.71PNIQPARR90 pKa = 11.84NSKK93 pKa = 10.03QVLDD97 pKa = 4.6YY98 pKa = 10.62ISKK101 pKa = 11.02DD102 pKa = 3.01GDD104 pKa = 3.15IKK106 pKa = 10.39TRR108 pKa = 11.84GDD110 pKa = 3.45FRR112 pKa = 11.84DD113 pKa = 3.79HH114 pKa = 7.01KK115 pKa = 10.4ISPSKK120 pKa = 10.85SDD122 pKa = 3.27ARR124 pKa = 11.84WRR126 pKa = 11.84TIIQTATTKK135 pKa = 10.79EE136 pKa = 4.21EE137 pKa = 3.97YY138 pKa = 10.81LDD140 pKa = 3.86MIKK143 pKa = 10.68EE144 pKa = 4.22EE145 pKa = 5.22FPHH148 pKa = 6.19EE149 pKa = 4.13WATKK153 pKa = 8.91LQWLEE158 pKa = 3.62YY159 pKa = 9.89SANKK163 pKa = 9.86LFPPQPEE170 pKa = 4.52PYY172 pKa = 9.63VSPFTEE178 pKa = 3.78SDD180 pKa = 3.68LRR182 pKa = 11.84CHH184 pKa = 6.88EE185 pKa = 5.67DD186 pKa = 2.87LAAWRR191 pKa = 11.84DD192 pKa = 3.17KK193 pKa = 11.02HH194 pKa = 6.79LYY196 pKa = 9.05HH197 pKa = 7.98VSIDD201 pKa = 3.24AYY203 pKa = 9.26TYY205 pKa = 8.99IHH207 pKa = 5.86TVSYY211 pKa = 9.8EE212 pKa = 3.98QAEE215 pKa = 4.28SDD217 pKa = 4.41LDD219 pKa = 3.52WMADD223 pKa = 3.35LTRR226 pKa = 11.84TMEE229 pKa = 4.45GLGSDD234 pKa = 4.04TPVSTSADD242 pKa = 3.27QLVPEE247 pKa = 5.18RR248 pKa = 11.84PPGLEE253 pKa = 3.87VSDD256 pKa = 4.08DD257 pKa = 3.7TTTGMVPSISQRR269 pKa = 11.84TMTMPPTTSSTTSPSSSSHH288 pKa = 6.34CGSSS292 pKa = 2.91

Molecular weight: 33.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8K7V5|F8K7V5_9GEMI Replication-associated protein (Fragment) OS=Chickpea chlorotic dwarf virus OX=463360 GN=repB PE=4 SV=1
MM1 pKa = 7.12STSTWGKK8 pKa = 8.27KK9 pKa = 8.03RR10 pKa = 11.84KK11 pKa = 9.59RR12 pKa = 11.84SGAEE16 pKa = 3.43SRR18 pKa = 11.84AKK20 pKa = 10.38SRR22 pKa = 11.84ASGSYY27 pKa = 9.01VPRR30 pKa = 11.84SVTSRR35 pKa = 11.84RR36 pKa = 11.84EE37 pKa = 3.96SLQVATFSWTSSGSGIKK54 pKa = 10.49FSAGGAAYY62 pKa = 9.97LVSNFPQGANDD73 pKa = 3.63NCRR76 pKa = 11.84HH77 pKa = 4.98TNKK80 pKa = 9.3TVLYY84 pKa = 10.59KK85 pKa = 10.37FMAKK89 pKa = 8.15NTIYY93 pKa = 10.54LDD95 pKa = 3.32SSHH98 pKa = 6.16YY99 pKa = 10.86SKK101 pKa = 11.11VFRR104 pKa = 11.84CPFTFWLVYY113 pKa = 10.72DD114 pKa = 4.55KK115 pKa = 11.51APGANVPSTSDD126 pKa = 2.85IFEE129 pKa = 4.59GPSLFPNNPWTWTVSRR145 pKa = 11.84AACHH149 pKa = 5.68RR150 pKa = 11.84FVVKK154 pKa = 9.21KK155 pKa = 8.29TWSCTVEE162 pKa = 4.23SNGIDD167 pKa = 3.36PGKK170 pKa = 10.57AQGSTYY176 pKa = 10.37YY177 pKa = 11.11GPGPCNQVKK186 pKa = 10.24SCNKK190 pKa = 8.07FFKK193 pKa = 10.55RR194 pKa = 11.84LGVSTEE200 pKa = 3.87WKK202 pKa = 10.02NSSTGDD208 pKa = 3.25VGDD211 pKa = 3.7IKK213 pKa = 10.92EE214 pKa = 4.06GALYY218 pKa = 10.17IVGAPSQKK226 pKa = 10.34SDD228 pKa = 3.54VYY230 pKa = 9.4VNGYY234 pKa = 8.88FRR236 pKa = 11.84VYY238 pKa = 10.14FKK240 pKa = 11.17SVGNQQ245 pKa = 2.8

Molecular weight: 27.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

769

92

292

192.3

21.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.072 ± 0.789

1.821 ± 0.446

6.372 ± 1.125

3.771 ± 1.022

5.202 ± 0.345

6.112 ± 1.393

2.731 ± 0.68

4.681 ± 1.088

6.372 ± 0.795

5.722 ± 1.156

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.56 ± 0.368

4.031 ± 0.976

6.502 ± 0.732

3.771 ± 0.606

4.811 ± 0.205

10.663 ± 1.177

8.062 ± 0.599

5.852 ± 1.613

2.211 ± 0.427

4.681 ± 0.338
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski