Escherichia phage EcoDS1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Kayfunavirus; Escherichia virus EcoDS1

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B3VCS6|B3VCS6_9CAUD Gp0.35 OS=Escherichia phage EcoDS1 OX=532075 GN=0.35 PE=4 SV=1
MM1 pKa = 7.37EE2 pKa = 5.49RR3 pKa = 11.84NANAYY8 pKa = 10.1YY9 pKa = 10.68DD10 pKa = 4.19LLAATVEE17 pKa = 4.23AFNEE21 pKa = 4.27RR22 pKa = 11.84IQYY25 pKa = 10.74DD26 pKa = 4.22KK27 pKa = 10.88IAEE30 pKa = 4.22GDD32 pKa = 3.88DD33 pKa = 3.56YY34 pKa = 11.62HH35 pKa = 7.46DD36 pKa = 4.53ALHH39 pKa = 6.27EE40 pKa = 4.34VVDD43 pKa = 4.59GQVPHH48 pKa = 6.81YY49 pKa = 8.66YY50 pKa = 10.32HH51 pKa = 7.62EE52 pKa = 4.81IFTVMAADD60 pKa = 5.4GIDD63 pKa = 3.84HH64 pKa = 6.56EE65 pKa = 5.23FEE67 pKa = 5.43DD68 pKa = 4.71SGLMPDD74 pKa = 3.68TKK76 pKa = 10.9DD77 pKa = 3.13VTRR80 pKa = 11.84ILQARR85 pKa = 11.84IYY87 pKa = 8.84EE88 pKa = 4.05ALYY91 pKa = 10.8NDD93 pKa = 3.95VSNSSDD99 pKa = 3.73VVWFEE104 pKa = 4.33PEE106 pKa = 3.84EE107 pKa = 4.28DD108 pKa = 4.04CEE110 pKa = 4.17

Molecular weight: 12.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B3VCU7|B3VCU7_9CAUD Gp4 OS=Escherichia phage EcoDS1 OX=532075 GN=4 PE=4 SV=1
MM1 pKa = 7.46IKK3 pKa = 10.37FIEE6 pKa = 4.24FLGRR10 pKa = 11.84LVVRR14 pKa = 11.84GYY16 pKa = 10.67RR17 pKa = 11.84RR18 pKa = 11.84AATMEE23 pKa = 4.03RR24 pKa = 11.84KK25 pKa = 9.11VARR28 pKa = 11.84EE29 pKa = 3.87DD30 pKa = 3.58CC31 pKa = 4.33

Molecular weight: 3.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12023

30

1295

226.8

25.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.55 ± 0.434

1.031 ± 0.194

6.429 ± 0.299

6.671 ± 0.394

3.585 ± 0.187

7.768 ± 0.367

1.713 ± 0.221

5.124 ± 0.212

6.671 ± 0.424

7.893 ± 0.286

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.911 ± 0.174

4.516 ± 0.403

3.735 ± 0.177

3.834 ± 0.43

5.431 ± 0.21

6.288 ± 0.376

5.93 ± 0.262

7.02 ± 0.267

1.464 ± 0.133

3.435 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski