Amino acid dipepetide frequency for Rhodothermaceae bacterium RA

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.647AlaAla: 14.647 ± 0.167	0.917AlaCys: 0.917 ± 0.027	6.769AlaAsp: 6.769 ± 0.076	7.378AlaGlu: 7.378 ± 0.102	4.596AlaPhe: 4.596 ± 0.061	9.961AlaGly: 9.961 ± 0.103	2.559AlaHis: 2.559 ± 0.052	4.124AlaIle: 4.124 ± 0.067	1.33AlaLys: 1.33 ± 0.041	13.386AlaLeu: 13.386 ± 0.173	2.317AlaMet: 2.317 ± 0.047	2.02AlaAsn: 2.02 ± 0.039	6.127AlaPro: 6.127 ± 0.085	3.409AlaGln: 3.409 ± 0.046	9.911AlaArg: 9.911 ± 0.106	5.363AlaSer: 5.363 ± 0.079	5.932AlaThr: 5.932 ± 0.092	8.958AlaVal: 8.958 ± 0.097	1.817AlaTrp: 1.817 ± 0.038	3.619AlaTyr: 3.619 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	0.64CysAla: 0.64 ± 0.022	0.094CysCys: 0.094 ± 0.009	0.367CysAsp: 0.367 ± 0.018	0.352CysGlu: 0.352 ± 0.014	0.257CysPhe: 0.257 ± 0.013	0.638CysGly: 0.638 ± 0.023	0.199CysHis: 0.199 ± 0.015	0.297CysIle: 0.297 ± 0.015	0.109CysLys: 0.109 ± 0.009	0.626CysLeu: 0.626 ± 0.021	0.099CysMet: 0.099 ± 0.008	0.158CysAsn: 0.158 ± 0.012	0.374CysPro: 0.374 ± 0.019	0.155CysGln: 0.155 ± 0.01	0.566CysArg: 0.566 ± 0.021	0.37CysSer: 0.37 ± 0.018	0.42CysThr: 0.42 ± 0.02	0.443CysVal: 0.443 ± 0.02	0.106CysTrp: 0.106 ± 0.009	0.177CysTyr: 0.177 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.495AspAla: 7.495 ± 0.087	0.215AspCys: 0.215 ± 0.015	3.517AspAsp: 3.517 ± 0.059	4.005AspGlu: 4.005 ± 0.066	2.094AspPhe: 2.094 ± 0.041	6.19AspGly: 6.19 ± 0.104	1.244AspHis: 1.244 ± 0.032	1.908AspIle: 1.908 ± 0.046	0.833AspLys: 0.833 ± 0.032	7.02AspLeu: 7.02 ± 0.094	0.718AspMet: 0.718 ± 0.025	1.067AspAsn: 1.067 ± 0.031	4.479AspPro: 4.479 ± 0.064	1.578AspGln: 1.578 ± 0.035	5.137AspArg: 5.137 ± 0.068	1.985AspSer: 1.985 ± 0.045	3.04AspThr: 3.04 ± 0.059	5.256AspVal: 5.256 ± 0.07	0.902AspTrp: 0.902 ± 0.028	1.726AspTyr: 1.726 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	9.975GluAla: 9.975 ± 0.124	0.214GluCys: 0.214 ± 0.012	3.431GluAsp: 3.431 ± 0.061	3.979GluGlu: 3.979 ± 0.074	1.333GluPhe: 1.333 ± 0.028	4.752GluGly: 4.752 ± 0.065	1.56GluHis: 1.56 ± 0.04	2.728GluIle: 2.728 ± 0.054	1.375GluLys: 1.375 ± 0.043	4.725GluLeu: 4.725 ± 0.073	1.222GluMet: 1.222 ± 0.032	1.338GluAsn: 1.338 ± 0.036	3.614GluPro: 3.614 ± 0.061	2.896GluGln: 2.896 ± 0.054	5.843GluArg: 5.843 ± 0.079	1.94GluSer: 1.94 ± 0.043	3.286GluThr: 3.286 ± 0.053	4.945GluVal: 4.945 ± 0.068	0.642GluTrp: 0.642 ± 0.023	1.243GluTyr: 1.243 ± 0.033	0.0GluXaa: 0.0 ± 0.0
Phe	3.549PheAla: 3.549 ± 0.051	0.251PheCys: 0.251 ± 0.013	2.886PheAsp: 2.886 ± 0.053	2.578PheGlu: 2.578 ± 0.043	1.629PhePhe: 1.629 ± 0.042	3.386PheGly: 3.386 ± 0.051	0.708PheHis: 0.708 ± 0.024	1.497PheIle: 1.497 ± 0.039	0.681PheLys: 0.681 ± 0.026	3.432PheLeu: 3.432 ± 0.062	0.628PheMet: 0.628 ± 0.023	1.083PheAsn: 1.083 ± 0.034	1.642PhePro: 1.642 ± 0.033	1.131PheGln: 1.131 ± 0.03	2.798PheArg: 2.798 ± 0.049	2.028PheSer: 2.028 ± 0.042	2.292PheThr: 2.292 ± 0.055	2.904PheVal: 2.904 ± 0.052	0.568PheTrp: 0.568 ± 0.021	1.196PheTyr: 1.196 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	7.986GlyAla: 7.986 ± 0.09	0.741GlyCys: 0.741 ± 0.024	4.503GlyAsp: 4.503 ± 0.074	4.349GlyGlu: 4.349 ± 0.059	3.311GlyPhe: 3.311 ± 0.051	6.939GlyGly: 6.939 ± 0.12	1.847GlyHis: 1.847 ± 0.041	3.689GlyIle: 3.689 ± 0.059	1.793GlyLys: 1.793 ± 0.043	9.269GlyLeu: 9.269 ± 0.112	1.831GlyMet: 1.831 ± 0.04	1.953GlyAsn: 1.953 ± 0.048	3.964GlyPro: 3.964 ± 0.059	2.75GlyGln: 2.75 ± 0.049	7.084GlyArg: 7.084 ± 0.08	4.0GlySer: 4.0 ± 0.07	5.969GlyThr: 5.969 ± 0.122	6.319GlyVal: 6.319 ± 0.07	1.412GlyTrp: 1.412 ± 0.037	2.798GlyTyr: 2.798 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	2.483HisAla: 2.483 ± 0.043	0.146HisCys: 0.146 ± 0.009	1.263HisAsp: 1.263 ± 0.032	1.277HisGlu: 1.277 ± 0.034	0.904HisPhe: 0.904 ± 0.025	2.0HisGly: 2.0 ± 0.042	0.719HisHis: 0.719 ± 0.025	0.871HisIle: 0.871 ± 0.026	0.33HisLys: 0.33 ± 0.015	2.908HisLeu: 2.908 ± 0.06	0.324HisMet: 0.324 ± 0.013	0.469HisAsn: 0.469 ± 0.021	1.938HisPro: 1.938 ± 0.047	0.641HisGln: 0.641 ± 0.021	2.219HisArg: 2.219 ± 0.049	0.736HisSer: 0.736 ± 0.023	1.322HisThr: 1.322 ± 0.037	1.845HisVal: 1.845 ± 0.043	0.324HisTrp: 0.324 ± 0.017	0.727HisTyr: 0.727 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	4.475IleAla: 4.475 ± 0.071	0.281IleCys: 0.281 ± 0.015	3.024IleAsp: 3.024 ± 0.057	3.2IleGlu: 3.2 ± 0.056	1.181IlePhe: 1.181 ± 0.035	3.842IleGly: 3.842 ± 0.07	0.9IleHis: 0.9 ± 0.031	1.543IleIle: 1.543 ± 0.049	0.902IleLys: 0.902 ± 0.028	3.667IleLeu: 3.667 ± 0.058	0.564IleMet: 0.564 ± 0.024	1.109IleAsn: 1.109 ± 0.031	2.345IlePro: 2.345 ± 0.047	1.364IleGln: 1.364 ± 0.037	3.47IleArg: 3.47 ± 0.054	1.814IleSer: 1.814 ± 0.04	2.458IleThr: 2.458 ± 0.048	3.268IleVal: 3.268 ± 0.063	0.437IleTrp: 0.437 ± 0.018	1.194IleTyr: 1.194 ± 0.032	0.0IleXaa: 0.0 ± 0.0
Lys	2.089LysAla: 2.089 ± 0.045	0.071LysCys: 0.071 ± 0.008	0.944LysAsp: 0.944 ± 0.032	1.117LysGlu: 1.117 ± 0.035	0.441LysPhe: 0.441 ± 0.021	1.328LysGly: 1.328 ± 0.035	0.447LysHis: 0.447 ± 0.019	0.9LysIle: 0.9 ± 0.029	0.755LysLys: 0.755 ± 0.033	1.819LysLeu: 1.819 ± 0.043	0.438LysMet: 0.438 ± 0.016	0.523LysAsn: 0.523 ± 0.022	1.134LysPro: 1.134 ± 0.032	0.773LysGln: 0.773 ± 0.023	1.564LysArg: 1.564 ± 0.038	0.782LysSer: 0.782 ± 0.027	1.229LysThr: 1.229 ± 0.038	1.31LysVal: 1.31 ± 0.043	0.223LysTrp: 0.223 ± 0.012	0.524LysTyr: 0.524 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	12.75LeuAla: 12.75 ± 0.124	0.743LeuCys: 0.743 ± 0.027	6.936LeuAsp: 6.936 ± 0.086	6.227LeuGlu: 6.227 ± 0.082	3.932LeuPhe: 3.932 ± 0.063	8.277LeuGly: 8.277 ± 0.101	2.706LeuHis: 2.706 ± 0.051	4.343LeuIle: 4.343 ± 0.07	2.281LeuLys: 2.281 ± 0.039	11.908LeuLeu: 11.908 ± 0.148	1.968LeuMet: 1.968 ± 0.041	2.495LeuAsn: 2.495 ± 0.043	6.448LeuPro: 6.448 ± 0.081	3.712LeuGln: 3.712 ± 0.056	8.965LeuArg: 8.965 ± 0.093	5.168LeuSer: 5.168 ± 0.068	6.389LeuThr: 6.389 ± 0.092	8.037LeuVal: 8.037 ± 0.111	1.307LeuTrp: 1.307 ± 0.038	3.112LeuTyr: 3.112 ± 0.05	0.0LeuXaa: 0.0 ± 0.0
Met	2.195MetAla: 2.195 ± 0.055	0.081MetCys: 0.081 ± 0.008	1.03MetAsp: 1.03 ± 0.032	1.065MetGlu: 1.065 ± 0.029	0.383MetPhe: 0.383 ± 0.018	1.32MetGly: 1.32 ± 0.031	0.459MetHis: 0.459 ± 0.02	0.815MetIle: 0.815 ± 0.028	0.592MetLys: 0.592 ± 0.022	1.996MetLeu: 1.996 ± 0.045	0.464MetMet: 0.464 ± 0.02	0.601MetAsn: 0.601 ± 0.021	1.434MetPro: 1.434 ± 0.034	0.902MetGln: 0.902 ± 0.028	1.476MetArg: 1.476 ± 0.033	0.968MetSer: 0.968 ± 0.032	1.337MetThr: 1.337 ± 0.033	1.215MetVal: 1.215 ± 0.028	0.158MetTrp: 0.158 ± 0.01	0.359MetTyr: 0.359 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	2.324AsnAla: 2.324 ± 0.044	0.131AsnCys: 0.131 ± 0.012	1.335AsnAsp: 1.335 ± 0.037	1.241AsnGlu: 1.241 ± 0.034	0.72AsnPhe: 0.72 ± 0.025	2.057AsnGly: 2.057 ± 0.05	0.506AsnHis: 0.506 ± 0.021	0.952AsnIle: 0.952 ± 0.032	0.429AsnLys: 0.429 ± 0.019	2.524AsnLeu: 2.524 ± 0.05	0.36AsnMet: 0.36 ± 0.016	0.667AsnAsn: 0.667 ± 0.03	1.953AsnPro: 1.953 ± 0.041	0.737AsnGln: 0.737 ± 0.025	1.86AsnArg: 1.86 ± 0.04	0.833AsnSer: 0.833 ± 0.032	1.372AsnThr: 1.372 ± 0.038	1.869AsnVal: 1.869 ± 0.046	0.322AsnTrp: 0.322 ± 0.018	0.715AsnTyr: 0.715 ± 0.027	0.0AsnXaa: 0.0 ± 0.0
Pro	7.895ProAla: 7.895 ± 0.099	0.317ProCys: 0.317 ± 0.016	4.984ProAsp: 4.984 ± 0.079	4.666ProGlu: 4.666 ± 0.067	2.297ProPhe: 2.297 ± 0.049	5.559ProGly: 5.559 ± 0.071	1.341ProHis: 1.341 ± 0.037	2.159ProIle: 2.159 ± 0.035	0.772ProLys: 0.772 ± 0.023	5.431ProLeu: 5.431 ± 0.074	1.12ProMet: 1.12 ± 0.028	1.4ProAsn: 1.4 ± 0.035	4.371ProPro: 4.371 ± 0.089	1.453ProGln: 1.453 ± 0.035	3.961ProArg: 3.961 ± 0.057	3.053ProSer: 3.053 ± 0.048	3.053ProThr: 3.053 ± 0.057	4.975ProVal: 4.975 ± 0.069	0.791ProTrp: 0.791 ± 0.027	1.682ProTyr: 1.682 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	4.647GlnAla: 4.647 ± 0.071	0.14GlnCys: 0.14 ± 0.011	1.768GlnAsp: 1.768 ± 0.036	2.104GlnGlu: 2.104 ± 0.043	1.037GlnPhe: 1.037 ± 0.029	2.536GlnGly: 2.536 ± 0.054	0.819GlnHis: 0.819 ± 0.026	1.65GlnIle: 1.65 ± 0.038	0.7GlnLys: 0.7 ± 0.026	2.653GlnLeu: 2.653 ± 0.053	0.733GlnMet: 0.733 ± 0.026	0.785GlnAsn: 0.785 ± 0.026	2.087GlnPro: 2.087 ± 0.047	1.707GlnGln: 1.707 ± 0.068	2.908GlnArg: 2.908 ± 0.055	1.242GlnSer: 1.242 ± 0.035	2.107GlnThr: 2.107 ± 0.041	2.749GlnVal: 2.749 ± 0.049	0.406GlnTrp: 0.406 ± 0.017	0.877GlnTyr: 0.877 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	8.689ArgAla: 8.689 ± 0.093	0.541ArgCys: 0.541 ± 0.023	4.19ArgAsp: 4.19 ± 0.057	4.666ArgGlu: 4.666 ± 0.065	3.719ArgPhe: 3.719 ± 0.056	5.16ArgGly: 5.16 ± 0.074	2.281ArgHis: 2.281 ± 0.041	4.063ArgIle: 4.063 ± 0.06	1.642ArgLys: 1.642 ± 0.043	10.45ArgLeu: 10.45 ± 0.118	1.807ArgMet: 1.807 ± 0.037	1.768ArgAsn: 1.768 ± 0.042	5.268ArgPro: 5.268 ± 0.074	3.28ArgGln: 3.28 ± 0.06	8.006ArgArg: 8.006 ± 0.112	3.754ArgSer: 3.754 ± 0.066	4.777ArgThr: 4.777 ± 0.062	5.889ArgVal: 5.889 ± 0.069	1.439ArgTrp: 1.439 ± 0.035	2.886ArgTyr: 2.886 ± 0.052	0.0ArgXaa: 0.0 ± 0.0
Ser	4.493SerAla: 4.493 ± 0.053	0.302SerCys: 0.302 ± 0.017	2.495SerAsp: 2.495 ± 0.049	2.212SerGlu: 2.212 ± 0.048	1.94SerPhe: 1.94 ± 0.04	4.437SerGly: 4.437 ± 0.076	0.914SerHis: 0.914 ± 0.031	1.947SerIle: 1.947 ± 0.038	0.788SerLys: 0.788 ± 0.03	4.912SerLeu: 4.912 ± 0.077	1.026SerMet: 1.026 ± 0.028	1.136SerAsn: 1.136 ± 0.03	2.934SerPro: 2.934 ± 0.047	1.169SerGln: 1.169 ± 0.033	3.36SerArg: 3.36 ± 0.046	2.408SerSer: 2.408 ± 0.05	2.817SerThr: 2.817 ± 0.048	3.4SerVal: 3.4 ± 0.062	0.666SerTrp: 0.666 ± 0.025	1.408SerTyr: 1.408 ± 0.039	0.0SerXaa: 0.0 ± 0.0
Thr	6.232ThrAla: 6.232 ± 0.08	0.39ThrCys: 0.39 ± 0.02	3.421ThrAsp: 3.421 ± 0.063	2.94ThrGlu: 2.94 ± 0.046	2.474ThrPhe: 2.474 ± 0.054	5.281ThrGly: 5.281 ± 0.084	1.314ThrHis: 1.314 ± 0.034	2.561ThrIle: 2.561 ± 0.047	0.871ThrLys: 0.871 ± 0.026	7.083ThrLeu: 7.083 ± 0.098	0.976ThrMet: 0.976 ± 0.028	1.338ThrAsn: 1.338 ± 0.038	4.216ThrPro: 4.216 ± 0.06	1.417ThrGln: 1.417 ± 0.029	4.199ThrArg: 4.199 ± 0.061	2.841ThrSer: 2.841 ± 0.059	3.612ThrThr: 3.612 ± 0.072	4.795ThrVal: 4.795 ± 0.074	0.93ThrTrp: 0.93 ± 0.035	2.062ThrTyr: 2.062 ± 0.047	0.0ThrXaa: 0.0 ± 0.0
Val	8.175ValAla: 8.175 ± 0.084	0.578ValCys: 0.578 ± 0.022	4.58ValAsp: 4.58 ± 0.065	5.065ValGlu: 5.065 ± 0.066	2.945ValPhe: 2.945 ± 0.045	5.43ValGly: 5.43 ± 0.071	1.77ValHis: 1.77 ± 0.038	3.288ValIle: 3.288 ± 0.057	1.355ValLys: 1.355 ± 0.036	9.02ValLeu: 9.02 ± 0.106	1.451ValMet: 1.451 ± 0.032	1.791ValAsn: 1.791 ± 0.04	4.674ValPro: 4.674 ± 0.066	2.947ValGln: 2.947 ± 0.056	6.757ValArg: 6.757 ± 0.082	3.592ValSer: 3.592 ± 0.056	4.67ValThr: 4.67 ± 0.069	6.515ValVal: 6.515 ± 0.087	1.051ValTrp: 1.051 ± 0.032	2.363ValTyr: 2.363 ± 0.049	0.0ValXaa: 0.0 ± 0.0
Trp	1.269TrpAla: 1.269 ± 0.033	0.094TrpCys: 0.094 ± 0.009	0.772TrpAsp: 0.772 ± 0.026	0.705TrpGlu: 0.705 ± 0.022	0.509TrpPhe: 0.509 ± 0.019	0.915TrpGly: 0.915 ± 0.031	0.413TrpHis: 0.413 ± 0.019	0.735TrpIle: 0.735 ± 0.026	0.349TrpLys: 0.349 ± 0.018	1.622TrpLeu: 1.622 ± 0.04	0.393TrpMet: 0.393 ± 0.015	0.451TrpAsn: 0.451 ± 0.02	0.689TrpPro: 0.689 ± 0.023	0.658TrpGln: 0.658 ± 0.025	1.167TrpArg: 1.167 ± 0.03	0.778TrpSer: 0.778 ± 0.031	1.004TrpThr: 1.004 ± 0.033	0.941TrpVal: 0.941 ± 0.036	0.26TrpTrp: 0.26 ± 0.015	0.447TrpTyr: 0.447 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.13TyrAla: 3.13 ± 0.045	0.198TyrCys: 0.198 ± 0.013	2.196TyrAsp: 2.196 ± 0.041	1.914TyrGlu: 1.914 ± 0.042	1.166TyrPhe: 1.166 ± 0.031	2.501TyrGly: 2.501 ± 0.05	0.739TyrHis: 0.739 ± 0.024	0.972TyrIle: 0.972 ± 0.029	0.524TyrLys: 0.524 ± 0.022	3.255TyrLeu: 3.255 ± 0.059	0.408TyrMet: 0.408 ± 0.02	0.755TyrAsn: 0.755 ± 0.028	1.588TyrPro: 1.588 ± 0.035	0.974TyrGln: 0.974 ± 0.028	3.025TyrArg: 3.025 ± 0.051	1.108TyrSer: 1.108 ± 0.031	1.866TyrThr: 1.866 ± 0.049	2.387TyrVal: 2.387 ± 0.046	0.411TyrTrp: 0.411 ± 0.022	1.002TyrTyr: 1.002 ± 0.036	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3503 proteins (1325449 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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