Maize Iranian mosaic nucleorhabdovirus
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B5FX46|B5FX46_9RHAB Large structural protein OS=Maize Iranian mosaic nucleorhabdovirus OX=348823 GN=L PE=4 SV=1
MM1 pKa = 7.19 ATSFLLLLSLSSILAISATSAPALTSGRR29 pKa = 11.84 IPRR32 pKa = 11.84 DD33 pKa = 3.48 EE34 pKa = 4.82 EE35 pKa = 4.03 PSSDD39 pKa = 4.43 KK40 pKa = 11.14 GDD42 pKa = 3.45 SDD44 pKa = 5.46 LYY46 pKa = 10.56 PLYY49 pKa = 10.72 EE50 pKa = 4.59 CGKK53 pKa = 9.3 EE54 pKa = 4.22 GTGVPITSWYY64 pKa = 8.14 GACRR68 pKa = 11.84 GACSMASNSTEE79 pKa = 3.97 VVAEE83 pKa = 4.01 IYY85 pKa = 10.23 YY86 pKa = 10.79 RR87 pKa = 11.84 NDD89 pKa = 2.83 SVGYY93 pKa = 9.8 IDD95 pKa = 4.01 VLSVRR100 pKa = 11.84 TTEE103 pKa = 3.87 IKK105 pKa = 9.38 KK106 pKa = 8.25 TSHH109 pKa = 4.34 VTWYY113 pKa = 9.65 GACDD117 pKa = 3.38 EE118 pKa = 4.3 EE119 pKa = 5.06 TIVGANSLAPEE130 pKa = 4.21 YY131 pKa = 10.32 IIEE134 pKa = 3.92 QSIEE138 pKa = 3.99 FLHH141 pKa = 6.8 SGLDD145 pKa = 3.29 SWQYY149 pKa = 9.94 GADD152 pKa = 3.06 IHH154 pKa = 6.42 VHH156 pKa = 5.18 DD157 pKa = 5.45 TIGFPDD163 pKa = 4.86 CDD165 pKa = 3.9 YY166 pKa = 11.75 LDD168 pKa = 4.58 DD169 pKa = 4.97 KK170 pKa = 11.82 NNEE173 pKa = 3.33 GWRR176 pKa = 11.84 FIITKK181 pKa = 10.08 RR182 pKa = 11.84 AIEE185 pKa = 4.38 LKK187 pKa = 10.54 SDD189 pKa = 3.2 IAGEE193 pKa = 4.45 GYY195 pKa = 10.04 IVDD198 pKa = 4.36 PDD200 pKa = 3.69 LGYY203 pKa = 11.12 VFPVSDD209 pKa = 4.55 GIGRR213 pKa = 11.84 GKK215 pKa = 10.16 FWYY218 pKa = 8.42 IWKK221 pKa = 10.16 SSSVPSGGCFFKK233 pKa = 11.06 SAGTGNCTLLMDD245 pKa = 4.65 TFTYY249 pKa = 10.26 SCPEE253 pKa = 3.97 LNVAFSAKK261 pKa = 10.31 VNTKK265 pKa = 10.83 LEE267 pKa = 4.41 GTCVGDD273 pKa = 4.8 LNISSDD279 pKa = 3.63 GVSYY283 pKa = 11.17 ALRR286 pKa = 11.84 SQQSTGSVSSALINLWHH303 pKa = 6.25 QSQEE307 pKa = 3.86 AVTQQLILVINDD319 pKa = 3.54 ALGKK323 pKa = 10.03 IEE325 pKa = 4.86 SSYY328 pKa = 11.64 CEE330 pKa = 3.95 ASCDD334 pKa = 3.39 LTEE337 pKa = 4.0 ALFYY341 pKa = 10.77 RR342 pKa = 11.84 VTDD345 pKa = 3.64 NPAVVEE351 pKa = 4.51 TPVGPWLPSLSKK363 pKa = 10.7 GKK365 pKa = 10.71 VSIIPCQSEE374 pKa = 3.85 QQLIIILPIEE384 pKa = 3.95 ICYY387 pKa = 10.18 EE388 pKa = 3.76 PFMLKK393 pKa = 10.11 VKK395 pKa = 9.82 SLKK398 pKa = 9.84 TGEE401 pKa = 4.41 TFWWLPSHH409 pKa = 5.91 SHH411 pKa = 4.64 VTSSTSCRR419 pKa = 11.84 SIATEE424 pKa = 3.59 EE425 pKa = 4.4 VEE427 pKa = 4.95 FQGEE431 pKa = 4.33 LKK433 pKa = 10.75 KK434 pKa = 10.61 PLTFEE439 pKa = 4.61 FWRR442 pKa = 11.84 GMYY445 pKa = 10.29 LLPYY449 pKa = 9.05 PYY451 pKa = 9.65 NGSGTWIDD459 pKa = 3.49 NPGTHH464 pKa = 6.11 IHH466 pKa = 7.04 RR467 pKa = 11.84 SSKK470 pKa = 9.23 WFPSINQLSYY480 pKa = 11.1 SVPIKK485 pKa = 10.75 LPLITKK491 pKa = 7.95 EE492 pKa = 3.78 IRR494 pKa = 11.84 KK495 pKa = 8.68 HH496 pKa = 4.24 VQQTVVSIGGFSNITSHH513 pKa = 7.74 PITEE517 pKa = 5.15 GIWTALSTAGLIIGKK532 pKa = 7.41 TAARR536 pKa = 11.84 VVMWWSEE543 pKa = 3.92 LEE545 pKa = 4.23 EE546 pKa = 4.25 TVKK549 pKa = 11.04 GYY551 pKa = 8.25 VTLAGGVIISLLVGYY566 pKa = 10.71 LMLKK570 pKa = 9.79 TILLTRR576 pKa = 11.84 RR577 pKa = 11.84 PSYY580 pKa = 10.16 SAVPRR585 pKa = 11.84 EE586 pKa = 4.41 VNWITPAKK594 pKa = 10.22
Molecular weight: 65.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.994
IPC2_protein 5.143
IPC_protein 5.105
Toseland 5.092
ProMoST 5.258
Dawson 5.118
Bjellqvist 5.245
Wikipedia 4.991
Rodwell 5.054
Grimsley 5.029
Solomon 5.118
Lehninger 5.08
Nozaki 5.258
DTASelect 5.385
Thurlkill 5.105
EMBOSS 5.054
Sillero 5.334
Patrickios 3.363
IPC_peptide 5.13
IPC2_peptide 5.334
IPC2.peptide.svr19 5.246
Protein with the highest isoelectric point:
>tr|B5FX42|B5FX42_9RHAB Phosphoprotein OS=Maize Iranian mosaic nucleorhabdovirus OX=348823 GN=P PE=4 SV=1
MM1 pKa = 7.65 ASITIPVEE9 pKa = 3.91 QVTKK13 pKa = 10.7 FSDD16 pKa = 3.71 DD17 pKa = 3.69 VKK19 pKa = 11.44 SLTQKK24 pKa = 10.69 ASEE27 pKa = 4.25 IPSSKK32 pKa = 10.64 SIIPQTEE39 pKa = 3.78 FSDD42 pKa = 4.63 AEE44 pKa = 4.13 LKK46 pKa = 9.29 KK47 pKa = 9.02 TLKK50 pKa = 9.79 FWEE53 pKa = 4.48 VTAMDD58 pKa = 5.04 DD59 pKa = 3.87 ATMVTQWSGVQSALAGGTFSAQNLKK84 pKa = 9.77 EE85 pKa = 3.98 LCQLAFNLRR94 pKa = 11.84 KK95 pKa = 8.87 PHH97 pKa = 6.15 EE98 pKa = 4.77 PGHH101 pKa = 5.53 VFIHH105 pKa = 6.76 NIPDD109 pKa = 2.81 SWKK112 pKa = 10.42 AYY114 pKa = 10.62 LEE116 pKa = 4.19 AATPDD121 pKa = 3.46 GTAIPATEE129 pKa = 4.38 NEE131 pKa = 4.52 STMSTIGAAAATGSADD147 pKa = 3.07 EE148 pKa = 4.21 TAPNKK153 pKa = 9.95 AAAIAFLSCTLMRR166 pKa = 11.84 LAIKK170 pKa = 9.68 EE171 pKa = 4.39 PKK173 pKa = 10.03 HH174 pKa = 8.42 IMDD177 pKa = 4.06 AMTNIRR183 pKa = 11.84 SRR185 pKa = 11.84 YY186 pKa = 7.21 GSLYY190 pKa = 9.97 GRR192 pKa = 11.84 SSPFLNSVTFNLSQLSRR209 pKa = 11.84 IKK211 pKa = 10.57 QGLEE215 pKa = 3.73 TYY217 pKa = 10.45 AVARR221 pKa = 11.84 GTLFYY226 pKa = 9.17 YY227 pKa = 9.91 TRR229 pKa = 11.84 HH230 pKa = 6.24 ADD232 pKa = 3.05 STYY235 pKa = 9.93 TYY237 pKa = 7.52 TTPSYY242 pKa = 10.34 GICRR246 pKa = 11.84 YY247 pKa = 10.24 LLFQHH252 pKa = 6.92 LEE254 pKa = 4.07 LEE256 pKa = 4.52 GMHH259 pKa = 6.77 IYY261 pKa = 11.03 KK262 pKa = 9.46 MLLALQTEE270 pKa = 4.93 WATVPMRR277 pKa = 11.84 LLLTWIRR284 pKa = 11.84 NPRR287 pKa = 11.84 SKK289 pKa = 10.7 LAVLEE294 pKa = 3.97 IVKK297 pKa = 10.25 ILTNWDD303 pKa = 3.3 KK304 pKa = 12.01 ANVDD308 pKa = 4.02 KK309 pKa = 10.95 GWKK312 pKa = 8.36 YY313 pKa = 11.18 ARR315 pKa = 11.84 LVNNTFFLNLSSRR328 pKa = 11.84 RR329 pKa = 11.84 NTYY332 pKa = 10.31 LCAVLAALNKK342 pKa = 10.06 KK343 pKa = 9.01 FVPQGTGDD351 pKa = 3.54 YY352 pKa = 11.05 ANPNNIAVIKK362 pKa = 11.03 NMDD365 pKa = 3.86 SAVKK369 pKa = 10.1 KK370 pKa = 10.17 QVSPDD375 pKa = 3.37 VQIVEE380 pKa = 4.8 RR381 pKa = 11.84 IYY383 pKa = 10.78 DD384 pKa = 3.45 IFLASSGSDD393 pKa = 3.3 DD394 pKa = 4.21 AGTAFTLSRR403 pKa = 11.84 GVKK406 pKa = 8.58 RR407 pKa = 11.84 TSPNNPEE414 pKa = 4.24 TRR416 pKa = 11.84 MQWTPTQEE424 pKa = 4.12 LEE426 pKa = 4.49 RR427 pKa = 11.84 DD428 pKa = 3.59 LEE430 pKa = 4.25 NPRR433 pKa = 11.84 LRR435 pKa = 11.84 RR436 pKa = 11.84 GSRR439 pKa = 11.84 IRR441 pKa = 11.84 EE442 pKa = 4.09 VNCRR446 pKa = 11.84 PEE448 pKa = 4.5 SDD450 pKa = 3.09 MSQMQRR456 pKa = 11.84 EE457 pKa = 4.57 LVFHH461 pKa = 6.58 EE462 pKa = 4.57 SVSVSILCDD471 pKa = 3.3 EE472 pKa = 4.59 GRR474 pKa = 11.84 VMSDD478 pKa = 3.7 PITRR482 pKa = 11.84 VSSIKK487 pKa = 10.75 CGVLLLL493 pKa = 4.39
Molecular weight: 55.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.88
IPC2_protein 8.068
IPC_protein 7.951
Toseland 8.009
ProMoST 8.478
Dawson 8.741
Bjellqvist 8.887
Wikipedia 8.756
Rodwell 8.814
Grimsley 7.966
Solomon 8.858
Lehninger 8.843
Nozaki 9.019
DTASelect 8.682
Thurlkill 8.77
EMBOSS 8.916
Sillero 9.019
Patrickios 4.571
IPC_peptide 8.843
IPC2_peptide 7.673
IPC2.peptide.svr19 7.795
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3798
233
1926
633.0
70.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.319 ± 0.634
1.606 ± 0.201
5.608 ± 0.56
6.135 ± 0.203
3.107 ± 0.264
6.214 ± 0.454
2.396 ± 0.268
7.399 ± 0.467
5.292 ± 0.237
9.9 ± 0.468
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.975 ± 0.449
3.923 ± 0.344
4.371 ± 0.469
2.87 ± 0.249
5.687 ± 0.69
8.557 ± 0.735
6.477 ± 0.756
6.214 ± 0.282
1.711 ± 0.283
3.239 ± 0.245
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here