Snakehead retrovirus
Average proteome isoelectric point is 7.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q88283|Q88283_9RETR Isoform of Q88282 Env protein OS=Snakehead retrovirus OX=40270 GN=env PE=4 SV=1
MM1 pKa = 7.15 ATSFKK6 pKa = 10.34 EE7 pKa = 4.13 PPGCGSDD14 pKa = 3.33 EE15 pKa = 5.34 DD16 pKa = 4.84 KK17 pKa = 11.69 DD18 pKa = 3.69 NDD20 pKa = 3.94 TPSRR24 pKa = 11.84 VPKK27 pKa = 9.62 PEE29 pKa = 3.9 EE30 pKa = 4.19 VISLYY35 pKa = 10.81 NPEE38 pKa = 3.94 LFAWGGTLGDD48 pKa = 4.1 YY49 pKa = 8.81 VWCKK53 pKa = 6.35 TTTIRR58 pKa = 11.84 WGPIVMKK65 pKa = 10.62 YY66 pKa = 9.47 RR67 pKa = 11.84 VCGHH71 pKa = 7.07 LEE73 pKa = 3.74 SDD75 pKa = 4.07 YY76 pKa = 8.72 TTSQYY81 pKa = 10.87 YY82 pKa = 9.43 RR83 pKa = 11.84 EE84 pKa = 4.64 SVNQTWIVGHH94 pKa = 5.79 SGRR97 pKa = 11.84 EE98 pKa = 3.9 VTHH101 pKa = 6.49 TCDD104 pKa = 3.76 HH105 pKa = 6.51 YY106 pKa = 11.31 CRR108 pKa = 11.84 CLNPPRR114 pKa = 11.84 FCWCRR119 pKa = 11.84 KK120 pKa = 7.54 HH121 pKa = 6.71 KK122 pKa = 10.0 RR123 pKa = 11.84 WHH125 pKa = 6.31 PFISPLGLVHH135 pKa = 6.68 WSWMEE140 pKa = 3.89 KK141 pKa = 9.88 FVQPVIRR148 pKa = 11.84 LEE150 pKa = 3.86 GWKK153 pKa = 9.72 EE154 pKa = 3.75 LKK156 pKa = 10.53 EE157 pKa = 4.05 EE158 pKa = 4.66 LLTHH162 pKa = 6.73 PDD164 pKa = 3.7 KK165 pKa = 11.21 IKK167 pKa = 8.96 IWVGRR172 pKa = 11.84 VHH174 pKa = 6.75 GRR176 pKa = 11.84 ISPDD180 pKa = 3.23 LSEE183 pKa = 5.56 HH184 pKa = 5.7 EE185 pKa = 5.12 LMVNMCAQGTLKK197 pKa = 9.4 PTLLPALWW205 pKa = 3.98
Molecular weight: 23.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.744
IPC2_protein 6.825
IPC_protein 6.868
Toseland 6.737
ProMoST 7.322
Dawson 7.468
Bjellqvist 7.614
Wikipedia 7.395
Rodwell 7.468
Grimsley 6.825
Solomon 7.512
Lehninger 7.541
Nozaki 7.966
DTASelect 7.688
Thurlkill 7.732
EMBOSS 7.746
Sillero 7.98
Patrickios 4.024
IPC_peptide 7.512
IPC2_peptide 7.351
IPC2.peptide.svr19 7.13
Protein with the highest isoelectric point:
>tr|Q88288|Q88288_9RETR Uncharacterized protein OS=Snakehead retrovirus OX=40270 PE=4 SV=1
MM1 pKa = 7.69 WDD3 pKa = 3.31 LWDD6 pKa = 4.57 LVPMDD11 pKa = 4.37 TEE13 pKa = 4.09 QALGGSWKK21 pKa = 10.06 SRR23 pKa = 11.84 KK24 pKa = 5.83 PTRR27 pKa = 11.84 RR28 pKa = 11.84 GLYY31 pKa = 8.8 RR32 pKa = 11.84 EE33 pKa = 3.76 TGEE36 pKa = 4.22 RR37 pKa = 11.84 HH38 pKa = 6.63 DD39 pKa = 4.09 GQIFQPIRR47 pKa = 11.84 NRR49 pKa = 11.84 SNQVHH54 pKa = 6.52 RR55 pKa = 11.84 PQPPRR60 pKa = 11.84 PTAPNPDD67 pKa = 3.19 NQRR70 pKa = 11.84 PIRR73 pKa = 11.84 EE74 pKa = 4.16 PRR76 pKa = 11.84 PGEE79 pKa = 4.12 PEE81 pKa = 4.14 HH82 pKa = 8.06 GDD84 pKa = 3.73 FLQGASWMWQQ94 pKa = 2.92
Molecular weight: 11.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.022
IPC2_protein 8.814
IPC_protein 9.326
Toseland 9.823
ProMoST 9.502
Dawson 9.984
Bjellqvist 9.663
Wikipedia 10.175
Rodwell 10.072
Grimsley 10.043
Solomon 10.16
Lehninger 10.145
Nozaki 9.736
DTASelect 9.677
Thurlkill 9.838
EMBOSS 10.204
Sillero 9.897
Patrickios 10.058
IPC_peptide 10.16
IPC2_peptide 8.331
IPC2.peptide.svr19 7.924
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
1
7
4192
52
2017
598.9
67.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.178 ± 0.905
1.956 ± 0.236
4.771 ± 0.359
6.751 ± 0.718
2.314 ± 0.184
8.111 ± 0.249
2.767 ± 0.351
4.222 ± 0.49
7.013 ± 0.812
8.564 ± 0.329
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.147 ± 0.115
3.793 ± 0.576
6.441 ± 0.517
5.94 ± 0.277
5.677 ± 0.502
5.248 ± 0.227
6.799 ± 0.519
5.725 ± 0.166
3.149 ± 0.207
2.409 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here