Bourbon virus
Average proteome isoelectric point is 7.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C5I425|A0A0C5I425_9ORTO Nucleoprotein OS=Bourbon virus OX=1618189 PE=4 SV=2
MM1 pKa = 7.41 EE2 pKa = 5.48 RR3 pKa = 11.84 FRR5 pKa = 11.84 PKK7 pKa = 10.13 TISTEE12 pKa = 3.16 IWQMSEE18 pKa = 5.28 HH19 pKa = 4.95 STEE22 pKa = 4.31 WISASSPHH30 pKa = 6.12 EE31 pKa = 3.98 MRR33 pKa = 11.84 ILEE36 pKa = 4.26 CVKK39 pKa = 9.97 YY40 pKa = 7.99 TVCCHH45 pKa = 6.09 ISDD48 pKa = 3.74 MHH50 pKa = 6.57 LHH52 pKa = 6.38 NGLPRR57 pKa = 11.84 YY58 pKa = 9.3 ILLTWCPKK66 pKa = 9.98 EE67 pKa = 4.3 VVDD70 pKa = 5.7 SVMRR74 pKa = 11.84 QNQMVFARR82 pKa = 11.84 PNIPDD87 pKa = 3.59 MLDD90 pKa = 3.12 LKK92 pKa = 10.0 TKK94 pKa = 10.48 LGLYY98 pKa = 9.28 VKK100 pKa = 10.01 IYY102 pKa = 10.24 TNEE105 pKa = 4.11 DD106 pKa = 3.03 QPKK109 pKa = 10.32 DD110 pKa = 3.61 YY111 pKa = 11.26 DD112 pKa = 3.72 SAPNEE117 pKa = 4.11 MILRR121 pKa = 11.84 MTTTGRR127 pKa = 11.84 FSPATLEE134 pKa = 3.99 YY135 pKa = 10.5 LDD137 pKa = 5.01 PSQSQDD143 pKa = 2.76 VRR145 pKa = 11.84 NFLVTFYY152 pKa = 11.31 NEE154 pKa = 4.09 CLEE157 pKa = 4.4 SNLYY161 pKa = 10.03 EE162 pKa = 5.02 RR163 pKa = 11.84 IARR166 pKa = 11.84 VQNEE170 pKa = 4.4 EE171 pKa = 4.18 PVPFAVPLTVEE182 pKa = 4.12 TLLLQDD188 pKa = 4.13 TDD190 pKa = 4.82 LDD192 pKa = 4.87 LIPNLEE198 pKa = 4.21 VSGTIPPVKK207 pKa = 9.91 GDD209 pKa = 3.03 RR210 pKa = 11.84 WSQMKK215 pKa = 8.4 TLPKK219 pKa = 10.28 LKK221 pKa = 9.89 TPIGRR226 pKa = 11.84 PAKK229 pKa = 9.39 WGAWLAGKK237 pKa = 7.5 EE238 pKa = 3.99 ARR240 pKa = 11.84 FKK242 pKa = 11.46 VFDD245 pKa = 3.9 EE246 pKa = 4.38 STDD249 pKa = 3.45 QVVAARR255 pKa = 11.84 FLADD259 pKa = 3.45 YY260 pKa = 9.81 TDD262 pKa = 4.02 TVCVRR267 pKa = 11.84 EE268 pKa = 4.2 SHH270 pKa = 6.08 ATPKK274 pKa = 10.33 TIIEE278 pKa = 4.33 TVLASLRR285 pKa = 11.84 AFSEE289 pKa = 4.21 GRR291 pKa = 11.84 PKK293 pKa = 9.56 RR294 pKa = 11.84 TKK296 pKa = 10.16 IQEE299 pKa = 4.0 DD300 pKa = 3.74 LQAFGIGIKK309 pKa = 9.71 KK310 pKa = 9.96 RR311 pKa = 11.84 KK312 pKa = 8.98 KK313 pKa = 8.61 QDD315 pKa = 2.99 EE316 pKa = 4.22 EE317 pKa = 4.26 VRR319 pKa = 11.84 LKK321 pKa = 11.2 SSVDD325 pKa = 2.83 DD326 pKa = 3.78 WKK328 pKa = 10.96 RR329 pKa = 11.84 ASFPMEE335 pKa = 3.91 EE336 pKa = 4.1 ASEE339 pKa = 4.36 PEE341 pKa = 4.12 WVVEE345 pKa = 3.87 EE346 pKa = 4.59 LEE348 pKa = 4.16 EE349 pKa = 4.72 LEE351 pKa = 5.28 RR352 pKa = 11.84 PTDD355 pKa = 4.01 MEE357 pKa = 4.69 WLTLDD362 pKa = 4.13 DD363 pKa = 5.17 NYY365 pKa = 10.71 IHH367 pKa = 6.96 TIVDD371 pKa = 3.22 EE372 pKa = 4.62 HH373 pKa = 6.25 ATEE376 pKa = 4.36 AVNSYY381 pKa = 10.9 KK382 pKa = 10.88 DD383 pKa = 3.26 IVTKK387 pKa = 10.55 LWVSALIEE395 pKa = 4.16 RR396 pKa = 11.84 MQVAATRR403 pKa = 11.84 AFNEE407 pKa = 3.99 LHH409 pKa = 6.17 TDD411 pKa = 3.46 RR412 pKa = 11.84 AKK414 pKa = 10.08 VTTVPIITRR423 pKa = 11.84 KK424 pKa = 8.09 SWKK427 pKa = 7.86 THH429 pKa = 5.28 SLSQLWGFVIVGPHH443 pKa = 6.8 HH444 pKa = 7.08 IKK446 pKa = 10.43 QEE448 pKa = 3.92 TDD450 pKa = 3.61 RR451 pKa = 11.84 IPIVTVEE458 pKa = 4.96 LVSEE462 pKa = 4.73 DD463 pKa = 3.82 NPEE466 pKa = 3.99 KK467 pKa = 10.82 YY468 pKa = 9.93 PNHH471 pKa = 5.58 SHH473 pKa = 7.63 FKK475 pKa = 10.02 FLYY478 pKa = 9.91 HH479 pKa = 7.14 KK480 pKa = 10.84 GNDD483 pKa = 3.99 SVGHH487 pKa = 6.32 EE488 pKa = 4.19 DD489 pKa = 3.29 VLVRR493 pKa = 11.84 VTSIAKK499 pKa = 9.62 YY500 pKa = 10.63 KK501 pKa = 10.73 LFTFSTIRR509 pKa = 11.84 RR510 pKa = 11.84 VYY512 pKa = 9.91 IQPCSVFSKK521 pKa = 11.1 LILKK525 pKa = 10.11 QSAEE529 pKa = 3.91 KK530 pKa = 10.44 RR531 pKa = 11.84 EE532 pKa = 4.02 KK533 pKa = 10.94 DD534 pKa = 3.35 FSLEE538 pKa = 3.68 NRR540 pKa = 11.84 IEE542 pKa = 3.73 IMINGRR548 pKa = 11.84 PVLVTWRR555 pKa = 11.84 SWIIRR560 pKa = 11.84 VLCIEE565 pKa = 4.39 YY566 pKa = 11.04 LMAIHH571 pKa = 6.66 NNPQMEE577 pKa = 4.36 GFLANIRR584 pKa = 11.84 RR585 pKa = 11.84 LHH587 pKa = 5.06 MARR590 pKa = 11.84 HH591 pKa = 5.08 AMMEE595 pKa = 4.16 KK596 pKa = 10.1 SCVFLPFGSAPEE608 pKa = 4.34 DD609 pKa = 3.36 KK610 pKa = 10.91 CNEE613 pKa = 4.24 CIINNPVVAFLARR626 pKa = 11.84 TWNEE630 pKa = 3.55 LPNVYY635 pKa = 8.89 MM636 pKa = 5.38
Molecular weight: 73.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.995
IPC2_protein 5.931
IPC_protein 5.995
Toseland 6.364
ProMoST 6.364
Dawson 6.287
Bjellqvist 6.287
Wikipedia 6.3
Rodwell 6.275
Grimsley 6.504
Solomon 6.287
Lehninger 6.287
Nozaki 6.561
DTASelect 6.751
Thurlkill 6.766
EMBOSS 6.737
Sillero 6.664
Patrickios 4.38
IPC_peptide 6.313
IPC2_peptide 6.605
IPC2.peptide.svr19 6.609
Protein with the highest isoelectric point:
>tr|A0A140H4W7|A0A140H4W7_9ORTO RNA-directed RNA polymerase catalytic subunit OS=Bourbon virus OX=1618189 GN=PB1 PE=4 SV=1
MM1 pKa = 7.68 EE2 pKa = 5.25 GNEE5 pKa = 3.93 TAKK8 pKa = 10.8 GIKK11 pKa = 10.1 ALIKK15 pKa = 10.2 KK16 pKa = 9.93 YY17 pKa = 10.43 KK18 pKa = 9.47 EE19 pKa = 3.98 LSNEE23 pKa = 3.7 PLFQEE28 pKa = 4.1 KK29 pKa = 10.4 RR30 pKa = 11.84 IWEE33 pKa = 3.95 YY34 pKa = 10.95 DD35 pKa = 3.16 TFKK38 pKa = 11.0 RR39 pKa = 11.84 YY40 pKa = 6.42 TTSKK44 pKa = 10.14 KK45 pKa = 10.26 DD46 pKa = 3.53 HH47 pKa = 6.77 APHH50 pKa = 6.07 TRR52 pKa = 11.84 LVYY55 pKa = 9.94 NVRR58 pKa = 11.84 RR59 pKa = 11.84 SYY61 pKa = 10.65 PLQVLPTLPEE71 pKa = 4.02 VYY73 pKa = 9.82 KK74 pKa = 10.25 GHH76 pKa = 6.88 RR77 pKa = 11.84 LCKK80 pKa = 9.7 RR81 pKa = 11.84 EE82 pKa = 3.66 VDD84 pKa = 3.03 IGEE87 pKa = 4.32 SKK89 pKa = 10.79 RR90 pKa = 11.84 ILGTIYY96 pKa = 10.87 CPDD99 pKa = 2.89 YY100 pKa = 10.82 WMSQGEE106 pKa = 4.27 PTSKK110 pKa = 10.53 EE111 pKa = 3.63 IAEE114 pKa = 4.22 SLIGPEE120 pKa = 3.73 MDD122 pKa = 3.04 KK123 pKa = 11.02 VDD125 pKa = 5.3 RR126 pKa = 11.84 FFQASWGDD134 pKa = 3.53 LQYY137 pKa = 11.79 GHH139 pKa = 7.93 LLPYY143 pKa = 9.67 RR144 pKa = 11.84 KK145 pKa = 8.81 PVSIVPVIQEE155 pKa = 3.92 VTPGSIAPTLEE166 pKa = 4.29 QVFCPQFSTIDD177 pKa = 3.14 AAGRR181 pKa = 11.84 SNRR184 pKa = 11.84 EE185 pKa = 3.75 AVEE188 pKa = 4.45 KK189 pKa = 9.01 ITKK192 pKa = 10.14 KK193 pKa = 10.02 IRR195 pKa = 11.84 PLLSLKK201 pKa = 8.83 STPALIHH208 pKa = 6.42 LARR211 pKa = 11.84 GLITQRR217 pKa = 11.84 KK218 pKa = 7.2 KK219 pKa = 9.68 WVPVTIDD226 pKa = 3.7 TNPSTAEE233 pKa = 3.84 LSHH236 pKa = 6.65 FLYY239 pKa = 10.57 SSYY242 pKa = 11.14 HH243 pKa = 5.89 HH244 pKa = 6.83 LPVSKK249 pKa = 10.65 VHH251 pKa = 6.25 TIEE254 pKa = 3.84 TASIEE259 pKa = 4.21 RR260 pKa = 11.84 LCGEE264 pKa = 4.32 IVQWALKK271 pKa = 8.13 TWEE274 pKa = 4.29 PKK276 pKa = 10.29 KK277 pKa = 10.79 KK278 pKa = 10.33 LLEE281 pKa = 4.28 LLEE284 pKa = 4.88 KK285 pKa = 9.76 ITVKK289 pKa = 9.69 GTSLSSILEE298 pKa = 4.08 SLDD301 pKa = 3.13 SDD303 pKa = 3.61 KK304 pKa = 11.27 QYY306 pKa = 11.49 TSICKK311 pKa = 9.31 VALGIPIHH319 pKa = 6.36 LVNQIRR325 pKa = 11.84 EE326 pKa = 4.06 TRR328 pKa = 11.84 FVIMNRR334 pKa = 11.84 EE335 pKa = 3.87 AEE337 pKa = 4.46 RR338 pKa = 11.84 INVEE342 pKa = 4.24 CIKK345 pKa = 10.81 KK346 pKa = 10.27 FGNIQYY352 pKa = 9.02 LHH354 pKa = 7.03 KK355 pKa = 9.56 YY356 pKa = 8.72 QRR358 pKa = 11.84 FEE360 pKa = 3.75 GPYY363 pKa = 9.91 KK364 pKa = 10.92 VFFHH368 pKa = 6.19 WRR370 pKa = 11.84 HH371 pKa = 4.47 VKK373 pKa = 10.65 GIIIGSDD380 pKa = 3.07 MKK382 pKa = 10.45 ISKK385 pKa = 9.94 ILLHH389 pKa = 6.89 AEE391 pKa = 3.83 QGDD394 pKa = 3.92 FLLPLVLDD402 pKa = 3.58 ILYY405 pKa = 8.23 YY406 pKa = 9.57 TASLEE411 pKa = 4.23 PGFEE415 pKa = 3.61 RR416 pKa = 11.84 SYY418 pKa = 11.51 DD419 pKa = 3.45 QFVEE423 pKa = 4.18 KK424 pKa = 10.72 KK425 pKa = 10.04 NLFSHH430 pKa = 7.11 FFAHH434 pKa = 6.23 HH435 pKa = 6.07 TSNPLEE441 pKa = 4.36 VYY443 pKa = 9.92 RR444 pKa = 11.84 VLGVGPTGALANSFHH459 pKa = 6.35 WKK461 pKa = 9.3 IVSEE465 pKa = 4.3 GFLSKK470 pKa = 10.0 IGEE473 pKa = 4.54 MEE475 pKa = 4.28 TSVQPYY481 pKa = 8.31 SAYY484 pKa = 10.54 AATHH488 pKa = 5.27 QLSDD492 pKa = 3.33 QLEE495 pKa = 4.15 VFRR498 pKa = 11.84 ISKK501 pKa = 9.96 HH502 pKa = 5.55 SKK504 pKa = 10.03 HH505 pKa = 6.57 KK506 pKa = 10.93 LIDD509 pKa = 4.2 PNEE512 pKa = 3.97 LLVPKK517 pKa = 10.03 TPLPLGLDD525 pKa = 3.4 ATSTEE530 pKa = 4.24 LVDD533 pKa = 5.34 VITNPLVKK541 pKa = 10.37 AKK543 pKa = 9.68 TMWTKK548 pKa = 11.17 LITDD552 pKa = 3.94 PMRR555 pKa = 11.84 CKK557 pKa = 10.2 TFVSNAITGNPDD569 pKa = 3.04 PFMKK573 pKa = 10.02 TVQQIMPPRR582 pKa = 11.84 SRR584 pKa = 11.84 VANKK588 pKa = 9.63 RR589 pKa = 11.84 RR590 pKa = 11.84 LEE592 pKa = 3.96 EE593 pKa = 4.35 LVEE596 pKa = 5.52 DD597 pKa = 4.36 DD598 pKa = 4.92 PNWTRR603 pKa = 11.84 SPEE606 pKa = 4.04 KK607 pKa = 10.14 RR608 pKa = 11.84 FKK610 pKa = 10.86 AAFDD614 pKa = 3.74 AAVLLAGKK622 pKa = 8.89 PKK624 pKa = 9.98 KK625 pKa = 10.18 RR626 pKa = 11.84 PAEE629 pKa = 4.2 GPPLNKK635 pKa = 9.72 VAMSCLGEE643 pKa = 4.07 TLDD646 pKa = 3.93 YY647 pKa = 11.44 SMSGGLVVSTGHH659 pKa = 7.54 KK660 pKa = 9.13 IRR662 pKa = 11.84 VMSQIVVDD670 pKa = 3.95 NSITPVEE677 pKa = 4.22 GFVYY681 pKa = 9.97 TGVYY685 pKa = 7.84 TEE687 pKa = 4.76 KK688 pKa = 10.45 PKK690 pKa = 11.25 DD691 pKa = 3.61 MEE693 pKa = 4.33 VTTLEE698 pKa = 4.46 DD699 pKa = 3.28 AMKK702 pKa = 9.87 MRR704 pKa = 11.84 LKK706 pKa = 10.44 RR707 pKa = 11.84 ICLMQHH713 pKa = 3.83 NRR715 pKa = 11.84 YY716 pKa = 8.92 YY717 pKa = 10.88 VLEE720 pKa = 4.52 EE721 pKa = 4.08 KK722 pKa = 10.24 TSLWSAIEE730 pKa = 4.12 NIRR733 pKa = 11.84 TTIDD737 pKa = 3.18 HH738 pKa = 5.37 QQSMIKK744 pKa = 10.4 ASQVDD749 pKa = 3.92 EE750 pKa = 4.6 ALLSLGAPGPSWKK763 pKa = 10.61
Molecular weight: 86.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.558
IPC2_protein 8.463
IPC_protein 8.317
Toseland 9.048
ProMoST 8.931
Dawson 9.37
Bjellqvist 9.165
Wikipedia 9.516
Rodwell 9.619
Grimsley 9.414
Solomon 9.399
Lehninger 9.355
Nozaki 9.282
DTASelect 9.077
Thurlkill 9.238
EMBOSS 9.516
Sillero 9.37
Patrickios 4.774
IPC_peptide 9.399
IPC2_peptide 7.732
IPC2.peptide.svr19 7.667
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3356
270
763
559.3
63.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.274 ± 0.255
2.056 ± 0.345
4.678 ± 0.24
7.449 ± 0.498
3.903 ± 0.313
5.334 ± 0.622
2.443 ± 0.246
5.423 ± 0.5
7.509 ± 0.484
9.803 ± 0.528
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.92 ± 0.297
4.112 ± 0.498
4.768 ± 0.557
3.725 ± 0.358
5.274 ± 0.41
7.33 ± 0.423
6.168 ± 0.268
6.943 ± 0.437
1.639 ± 0.259
3.248 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here