Circovirus-like genome SAR-A

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6GIJ1|C6GIJ1_9VIRU Putative Rep protein OS=Circovirus-like genome SAR-A OX=642258 PE=4 SV=2
MM1 pKa = 7.46PRR3 pKa = 11.84VNPAKK8 pKa = 10.21AWCFTLNNYY17 pKa = 7.62TEE19 pKa = 4.39NEE21 pKa = 3.98HH22 pKa = 6.14GALVQRR28 pKa = 11.84FSDD31 pKa = 4.35FDD33 pKa = 3.76DD34 pKa = 3.77KK35 pKa = 11.73YY36 pKa = 10.63YY37 pKa = 10.92FIVGCEE43 pKa = 3.74IGAQGTPHH51 pKa = 5.88LQGYY55 pKa = 8.38IEE57 pKa = 4.28KK58 pKa = 10.29KK59 pKa = 9.35VGRR62 pKa = 11.84FRR64 pKa = 11.84PLPCFEE70 pKa = 4.26VLRR73 pKa = 11.84DD74 pKa = 3.94GKK76 pKa = 10.51NAMHH80 pKa = 7.1FEE82 pKa = 4.1RR83 pKa = 11.84AKK85 pKa = 10.98GNRR88 pKa = 11.84KK89 pKa = 8.59QNYY92 pKa = 8.63NYY94 pKa = 9.91CSKK97 pKa = 11.13DD98 pKa = 2.75GDD100 pKa = 4.4FITNIDD106 pKa = 4.28KK107 pKa = 10.82PIMTYY112 pKa = 10.47SEE114 pKa = 5.15AKK116 pKa = 10.45DD117 pKa = 2.88IWKK120 pKa = 7.2EE121 pKa = 4.0TNGISNDD128 pKa = 3.39KK129 pKa = 10.58YY130 pKa = 11.09DD131 pKa = 3.5AATINEE137 pKa = 4.02AAMHH141 pKa = 6.35IEE143 pKa = 4.32YY144 pKa = 9.65MDD146 pKa = 5.72LYY148 pKa = 11.22DD149 pKa = 5.0CYY151 pKa = 10.82TDD153 pKa = 3.8EE154 pKa = 4.41GQKK157 pKa = 10.96KK158 pKa = 9.86FMVRR162 pKa = 11.84YY163 pKa = 8.79KK164 pKa = 11.52AMMQARR170 pKa = 11.84YY171 pKa = 8.83PEE173 pKa = 4.56KK174 pKa = 9.66EE175 pKa = 4.13TVEE178 pKa = 4.04II179 pKa = 4.53

Molecular weight: 20.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6GIJ1|C6GIJ1_9VIRU Putative Rep protein OS=Circovirus-like genome SAR-A OX=642258 PE=4 SV=2
MM1 pKa = 7.73PGYY4 pKa = 10.46NFRR7 pKa = 11.84SNPVKK12 pKa = 10.39KK13 pKa = 9.53PSYY16 pKa = 9.92RR17 pKa = 11.84FRR19 pKa = 11.84SKK21 pKa = 10.67SKK23 pKa = 10.17PRR25 pKa = 11.84SQPNKK30 pKa = 9.12SVKK33 pKa = 10.46KK34 pKa = 9.54EE35 pKa = 4.0VKK37 pKa = 10.47KK38 pKa = 9.42NTKK41 pKa = 9.7DD42 pKa = 3.1IKK44 pKa = 10.45LLKK47 pKa = 10.61SVGFQYY53 pKa = 11.34APFQQRR59 pKa = 11.84EE60 pKa = 4.05VGTISNFLHH69 pKa = 6.39TSLLTAPNNWGGIFRR84 pKa = 11.84MHH86 pKa = 6.53GVSNEE91 pKa = 3.89DD92 pKa = 3.5LPRR95 pKa = 11.84QYY97 pKa = 10.77MMKK100 pKa = 10.19SVDD103 pKa = 3.87VNWIAQAEE111 pKa = 4.02ASEE114 pKa = 4.64VGNLWLQVFVVSLKK128 pKa = 10.85HH129 pKa = 6.63KK130 pKa = 8.95MANQVLTRR138 pKa = 11.84TTRR141 pKa = 11.84LTNLEE146 pKa = 4.16EE147 pKa = 4.36GLDD150 pKa = 3.92YY151 pKa = 10.9TSQSAGTAFALQGDD165 pKa = 4.94LGYY168 pKa = 11.29KK169 pKa = 10.28LNPDD173 pKa = 4.51LYY175 pKa = 9.23TIHH178 pKa = 6.05YY179 pKa = 9.92HH180 pKa = 5.45SGQRR184 pKa = 11.84RR185 pKa = 11.84IGEE188 pKa = 4.4STVGEE193 pKa = 4.33SPVAVTNINNGTTMGSCRR211 pKa = 11.84IKK213 pKa = 10.23WPHH216 pKa = 5.1TFKK219 pKa = 11.07NDD221 pKa = 3.16EE222 pKa = 4.09TSDD225 pKa = 3.47AGFKK229 pKa = 10.49EE230 pKa = 3.87LTYY233 pKa = 11.16QNIEE237 pKa = 4.32PKK239 pKa = 9.51QHH241 pKa = 6.84LYY243 pKa = 10.79LLVMSNTSGSVITGGEE259 pKa = 4.06LFFSSRR265 pKa = 11.84AQFNGHH271 pKa = 5.29VNNPNN276 pKa = 3.03

Molecular weight: 31.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

455

179

276

227.5

26.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.495 ± 1.092

1.319 ± 0.911

4.615 ± 1.635

5.934 ± 1.166

4.835 ± 0.119

7.033 ± 0.548

2.637 ± 0.249

4.615 ± 0.945

7.912 ± 0.634

6.154 ± 1.731

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.297 ± 0.724

7.692 ± 0.611

4.396 ± 0.3

4.396 ± 0.645

4.835 ± 0.119

6.374 ± 2.557

6.374 ± 0.831

5.714 ± 1.114

1.319 ± 0.124

5.055 ± 1.364
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski