Aeromonas phage CF7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Melnykvirinae; Ahphunavirus; Aeromonas virus CF7

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A249XMX5|A0A249XMX5_9CAUD Uncharacterized protein OS=Aeromonas phage CF7 OX=2507411 GN=CF7_34 PE=4 SV=1
MM1 pKa = 6.73QTTLQYY7 pKa = 10.9LPSLSCGAALRR18 pKa = 11.84AAISRR23 pKa = 11.84VLDD26 pKa = 3.57YY27 pKa = 11.57ARR29 pKa = 11.84VNAQDD34 pKa = 4.91GSDD37 pKa = 3.72TPNLLDD43 pKa = 4.4EE44 pKa = 5.03FADD47 pKa = 3.88SQIALLDD54 pKa = 3.78VAHH57 pKa = 7.42GDD59 pKa = 3.63PADD62 pKa = 4.14GVWLGVGVPDD72 pKa = 5.62PRR74 pKa = 11.84TLCYY78 pKa = 10.01KK79 pKa = 10.32AASEE83 pKa = 4.08YY84 pKa = 10.84RR85 pKa = 11.84LTQSTSAKK93 pKa = 10.12SEE95 pKa = 4.44LISLYY100 pKa = 10.56TDD102 pKa = 3.63IKK104 pKa = 10.96NVAAQLPGFVAPEE117 pKa = 3.96VVVATTSLSADD128 pKa = 3.22GEE130 pKa = 4.55VPYY133 pKa = 11.19KK134 pKa = 10.7NGQPAA139 pKa = 2.96

Molecular weight: 14.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A249XLB1|A0A249XLB1_9CAUD Putative nucleotidyl transferase OS=Aeromonas phage CF7 OX=2507411 GN=CF7_47 PE=4 SV=1
MM1 pKa = 7.85NLQQWNAQQARR12 pKa = 11.84LRR14 pKa = 11.84HH15 pKa = 4.78EE16 pKa = 4.5EE17 pKa = 3.77KK18 pKa = 10.34RR19 pKa = 11.84IAGATPEE26 pKa = 3.96YY27 pKa = 10.16HH28 pKa = 7.01AAIQARR34 pKa = 11.84VDD36 pKa = 3.24RR37 pKa = 11.84ALAEE41 pKa = 4.03LAASQEE47 pKa = 4.43SRR49 pKa = 11.84GLRR52 pKa = 11.84HH53 pKa = 4.65TTGGDD58 pKa = 3.28FCHH61 pKa = 7.65LARR64 pKa = 11.84SLNTVSFRR72 pKa = 11.84RR73 pKa = 11.84GPKK76 pKa = 9.57

Molecular weight: 8.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

13203

60

1252

269.4

29.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.77 ± 0.62

1.227 ± 0.175

5.923 ± 0.174

6.052 ± 0.264

3.295 ± 0.18

7.998 ± 0.31

2.022 ± 0.195

4.196 ± 0.206

5.665 ± 0.3

9.165 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.908 ± 0.163

3.757 ± 0.191

3.999 ± 0.239

4.522 ± 0.342

5.961 ± 0.251

5.605 ± 0.361

5.521 ± 0.295

6.756 ± 0.349

1.341 ± 0.142

3.317 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski