Magnetospirillum sp. UT-4

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae; Magnetospirillum; unclassified Magnetospirillum

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4085 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J4FG57|A0A6J4FG57_9PROT Uncharacterized protein OS=Magnetospirillum sp. UT-4 OX=2681467 GN=MTBUT4_270014 PE=4 SV=1
MM1 pKa = 7.87AYY3 pKa = 10.28LSKK6 pKa = 10.73DD7 pKa = 3.61LSVLAYY13 pKa = 10.66ANGFTLWHH21 pKa = 5.97YY22 pKa = 6.54TTADD26 pKa = 2.99AAATVDD32 pKa = 3.13GSGYY36 pKa = 10.17FNPAADD42 pKa = 3.95MLRR45 pKa = 11.84AGDD48 pKa = 3.97IIIANVEE55 pKa = 4.13SGGTPKK61 pKa = 10.73AGLFFVSTAASGVVDD76 pKa = 4.41VNDD79 pKa = 3.53MTQIGATDD87 pKa = 3.35SDD89 pKa = 3.87

Molecular weight: 9.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J4FG62|A0A6J4FG62_9PROT ATPase associated with various cellular activities OS=Magnetospirillum sp. UT-4 OX=2681467 GN=MTBUT4_260069 PE=4 SV=1
MM1 pKa = 7.31GRR3 pKa = 11.84GARR6 pKa = 11.84RR7 pKa = 11.84AVARR11 pKa = 11.84HH12 pKa = 5.84RR13 pKa = 11.84GGGGGGDD20 pKa = 3.45EE21 pKa = 4.59PRR23 pKa = 11.84RR24 pKa = 11.84PGQGRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84PLLVGRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84HH41 pKa = 5.1RR42 pKa = 11.84VPAPVAVLVLEE53 pKa = 4.38RR54 pKa = 11.84EE55 pKa = 4.36RR56 pKa = 11.84PQFRR60 pKa = 11.84QMRR63 pKa = 11.84RR64 pKa = 11.84HH65 pKa = 5.3HH66 pKa = 6.13HH67 pKa = 6.17RR68 pKa = 11.84EE69 pKa = 3.42PRR71 pKa = 11.84FPPVRR76 pKa = 11.84AGGPPRR82 pKa = 11.84RR83 pKa = 11.84GRR85 pKa = 11.84GARR88 pKa = 11.84GPHH91 pKa = 6.2RR92 pKa = 11.84RR93 pKa = 11.84RR94 pKa = 11.84HH95 pKa = 5.02PLPRR99 pKa = 11.84ARR101 pKa = 11.84AVAALGARR109 pKa = 11.84SLAHH113 pKa = 5.97GRR115 pKa = 11.84HH116 pKa = 5.26RR117 pKa = 11.84QPPVLQRR124 pKa = 11.84RR125 pKa = 11.84GVRR128 pKa = 11.84TQPPSFRR135 pKa = 11.84RR136 pKa = 11.84NPAQLLSS143 pKa = 3.62

Molecular weight: 16.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4085

0

4085

1230408

20

12104

301.2

32.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.789 ± 0.084

0.962 ± 0.017

5.843 ± 0.037

5.703 ± 0.04

3.367 ± 0.025

8.954 ± 0.053

2.193 ± 0.022

4.209 ± 0.031

2.981 ± 0.038

10.372 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.498 ± 0.021

2.195 ± 0.034

5.519 ± 0.047

2.833 ± 0.021

7.845 ± 0.07

4.624 ± 0.038

4.944 ± 0.09

7.852 ± 0.036

1.349 ± 0.016

1.967 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski