Synechococcus phage S-N03

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 247 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G8R623|A0A6G8R623_9CAUD Uncharacterized protein OS=Synechococcus phage S-N03 OX=2718943 PE=4 SV=1
MM1 pKa = 7.75PRR3 pKa = 11.84RR4 pKa = 11.84SDD6 pKa = 3.58TSDD9 pKa = 3.2LTCALDD15 pKa = 3.9ANPVLDD21 pKa = 3.99RR22 pKa = 11.84DD23 pKa = 3.62NVYY26 pKa = 10.59FSDD29 pKa = 3.47QGWAYY34 pKa = 10.38RR35 pKa = 11.84HH36 pKa = 5.7YY37 pKa = 11.37KK38 pKa = 8.47NTTKK42 pKa = 10.49TEE44 pKa = 4.01FCDD47 pKa = 4.01EE48 pKa = 4.1ILVAGQALLDD58 pKa = 4.32NGDD61 pKa = 4.44PDD63 pKa = 3.81TTADD67 pKa = 3.72PFGTASPTFLVGDD80 pKa = 4.31SVQSPEE86 pKa = 5.09AGHH89 pKa = 5.92QPGDD93 pKa = 3.15GGAAVLALDD102 pKa = 3.87VTEE105 pKa = 4.89SGSGYY110 pKa = 8.95TGASGVTTTAITGSGTGLTVTTLDD134 pKa = 3.44AAGLLVTATIDD145 pKa = 3.45NGGDD149 pKa = 3.43GYY151 pKa = 11.32EE152 pKa = 4.19VGDD155 pKa = 3.92VVSVDD160 pKa = 3.16GGTGGEE166 pKa = 4.08LTITSVDD173 pKa = 3.51EE174 pKa = 4.18

Molecular weight: 17.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G8R625|A0A6G8R625_9CAUD Uncharacterized protein OS=Synechococcus phage S-N03 OX=2718943 PE=4 SV=1
MM1 pKa = 7.63AKK3 pKa = 8.39VTYY6 pKa = 10.15RR7 pKa = 11.84GVSYY11 pKa = 8.51DD12 pKa = 3.8TNRR15 pKa = 11.84HH16 pKa = 4.15QPTVRR21 pKa = 11.84KK22 pKa = 9.59VRR24 pKa = 11.84EE25 pKa = 3.91LIYY28 pKa = 10.53RR29 pKa = 11.84GIKK32 pKa = 10.05RR33 pKa = 11.84LVEE36 pKa = 4.29VKK38 pKa = 10.57

Molecular weight: 4.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

247

0

247

49727

29

2476

201.3

22.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.722 ± 0.201

0.901 ± 0.065

6.711 ± 0.161

6.733 ± 0.254

3.855 ± 0.11

7.837 ± 0.242

1.705 ± 0.099

5.245 ± 0.154

5.88 ± 0.371

7.672 ± 0.174

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.379 ± 0.119

5.084 ± 0.184

4.094 ± 0.132

3.869 ± 0.104

4.555 ± 0.156

6.451 ± 0.229

7.28 ± 0.366

6.719 ± 0.163

1.305 ± 0.08

4.004 ± 0.121
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski