Thamnophis sirtalis

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Lepidosauria; Squamata

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22897 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I9Y5M5|A0A6I9Y5M5_9SAUR Protein YIPF OS=Thamnophis sirtalis OX=35019 GN=YIPF2 PE=3 SV=1
MM1 pKa = 6.61FTAMCEE7 pKa = 4.31CQALHH12 pKa = 7.39PDD14 pKa = 4.25PEE16 pKa = 6.23DD17 pKa = 3.75EE18 pKa = 5.77DD19 pKa = 5.15SDD21 pKa = 4.07GNYY24 pKa = 10.49DD25 pKa = 3.54GEE27 pKa = 4.44EE28 pKa = 4.36YY29 pKa = 10.54DD30 pKa = 4.66VEE32 pKa = 4.1AHH34 pKa = 7.02EE35 pKa = 5.17IGQGDD40 pKa = 3.69IPSFYY45 pKa = 9.93TYY47 pKa = 11.02EE48 pKa = 4.2EE49 pKa = 4.34GLSRR53 pKa = 11.84LTAEE57 pKa = 4.45GQATLEE63 pKa = 4.11RR64 pKa = 11.84LEE66 pKa = 4.74GMLAQSVSTQYY77 pKa = 11.43HH78 pKa = 4.86MAGVRR83 pKa = 11.84TEE85 pKa = 3.63EE86 pKa = 4.02TLRR89 pKa = 11.84DD90 pKa = 3.84FEE92 pKa = 7.14DD93 pKa = 4.08GMEE96 pKa = 3.91VDD98 pKa = 4.13AAPVVTGQFEE108 pKa = 4.29DD109 pKa = 4.3ADD111 pKa = 4.0VEE113 pKa = 4.5HH114 pKa = 7.13

Molecular weight: 12.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I9YF16|A0A6I9YF16_9SAUR G protein-coupled receptor kinase OS=Thamnophis sirtalis OX=35019 GN=GRK5 PE=3 SV=1
MM1 pKa = 7.57SSHH4 pKa = 6.01KK5 pKa = 9.07TFKK8 pKa = 10.28IKK10 pKa = 10.6RR11 pKa = 11.84FLAKK15 pKa = 9.68KK16 pKa = 9.58QKK18 pKa = 8.69QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84MKK30 pKa = 9.89TGNKK34 pKa = 8.61IRR36 pKa = 11.84YY37 pKa = 7.09NSKK40 pKa = 8.3RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 3.95WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.83LGLL51 pKa = 3.67

Molecular weight: 6.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18522

4375

22897

12268805

37

34811

535.8

60.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.322 ± 0.015

2.194 ± 0.013

4.878 ± 0.011

7.28 ± 0.02

3.808 ± 0.013

5.885 ± 0.02

2.624 ± 0.008

5.051 ± 0.013

6.398 ± 0.019

9.707 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.313 ± 0.007

4.225 ± 0.011

5.634 ± 0.019

4.851 ± 0.017

5.156 ± 0.012

8.596 ± 0.021

5.38 ± 0.013

5.766 ± 0.013

1.133 ± 0.005

2.794 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski