Klebsiella phage K11
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B3VD14|B3VD14_9CAUD Gp12 OS=Klebsiella phage K11 OX=532077 GN=12 PE=4 SV=1
MM1 pKa = 7.38 NMQDD5 pKa = 4.11 AYY7 pKa = 10.62 FGSAAEE13 pKa = 4.04 LDD15 pKa = 4.97 AINEE19 pKa = 3.9 MLAAIGEE26 pKa = 4.7 SPVTTLDD33 pKa = 3.33 EE34 pKa = 5.18 DD35 pKa = 4.25 GSADD39 pKa = 3.27 VANARR44 pKa = 11.84 RR45 pKa = 11.84 ILNRR49 pKa = 11.84 INRR52 pKa = 11.84 QIQSKK57 pKa = 9.61 GWAFNINEE65 pKa = 4.55 SATLTPDD72 pKa = 3.54 ADD74 pKa = 3.61 TGLIPFRR81 pKa = 11.84 PAYY84 pKa = 10.44 LSILGGQYY92 pKa = 10.17 VNRR95 pKa = 11.84 GGWVYY100 pKa = 11.23 DD101 pKa = 3.54 KK102 pKa = 10.79 STEE105 pKa = 3.83 TDD107 pKa = 3.35 TFSGAITVTLITLQDD122 pKa = 3.68 YY123 pKa = 11.66 DD124 pKa = 4.32 EE125 pKa = 4.72 MPEE128 pKa = 5.08 CFRR131 pKa = 11.84 QWIVTKK137 pKa = 10.66 ASRR140 pKa = 11.84 QFNSRR145 pKa = 11.84 FFGAEE150 pKa = 3.73 DD151 pKa = 3.76 VEE153 pKa = 4.46 NSLAQEE159 pKa = 3.88 EE160 pKa = 4.75 MEE162 pKa = 5.66 ARR164 pKa = 11.84 MACNEE169 pKa = 3.84 YY170 pKa = 10.91 EE171 pKa = 4.14 MDD173 pKa = 3.84 FGQYY177 pKa = 11.01 NMLDD181 pKa = 3.06 GDD183 pKa = 4.58 AYY185 pKa = 10.18 VQGLIGRR192 pKa = 4.48
Molecular weight: 21.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.227
IPC2_protein 4.126
IPC_protein 4.088
Toseland 3.884
ProMoST 4.215
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.948
Rodwell 3.91
Grimsley 3.795
Solomon 4.037
Lehninger 3.999
Nozaki 4.151
DTASelect 4.342
Thurlkill 3.923
EMBOSS 3.961
Sillero 4.19
Patrickios 3.49
IPC_peptide 4.037
IPC2_peptide 4.177
IPC2.peptide.svr19 4.105
Protein with the highest isoelectric point:
>tr|B3VCY8|B3VCY8_9CAUD Gp1.7 OS=Klebsiella phage K11 OX=532077 GN=1.7 PE=4 SV=1
MM1 pKa = 7.88 RR2 pKa = 11.84 LHH4 pKa = 6.7 FNKK7 pKa = 10.46 SNGIFSVRR15 pKa = 11.84 RR16 pKa = 11.84 EE17 pKa = 4.04 DD18 pKa = 3.6 RR19 pKa = 11.84 STVAATEE26 pKa = 3.98 RR27 pKa = 11.84 HH28 pKa = 5.03 GKK30 pKa = 8.71 IPRR33 pKa = 11.84 IGDD36 pKa = 3.66 TFEE39 pKa = 4.6 LAPSVHH45 pKa = 6.56 ILVTRR50 pKa = 11.84 GLYY53 pKa = 9.93 EE54 pKa = 4.12 LAQTKK59 pKa = 9.86 SRR61 pKa = 11.84 PFVPVVVTKK70 pKa = 9.98 WPRR73 pKa = 11.84 LRR75 pKa = 11.84 LFWEE79 pKa = 4.85 RR80 pKa = 11.84 IKK82 pKa = 11.06 EE83 pKa = 4.25 VVNDD87 pKa = 3.83
Molecular weight: 10.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.663
IPC_protein 10.687
Toseland 11.038
ProMoST 11.023
Dawson 11.067
Bjellqvist 10.862
Wikipedia 11.359
Rodwell 11.169
Grimsley 11.096
Solomon 11.33
Lehninger 11.286
Nozaki 11.008
DTASelect 10.862
Thurlkill 11.023
EMBOSS 11.462
Sillero 11.023
Patrickios 10.935
IPC_peptide 11.345
IPC2_peptide 9.882
IPC2.peptide.svr19 8.922
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
51
0
51
13349
31
1321
261.7
29.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.944 ± 0.485
1.064 ± 0.164
6.188 ± 0.204
6.6 ± 0.397
3.603 ± 0.15
8.113 ± 0.326
1.745 ± 0.191
4.809 ± 0.164
6.405 ± 0.4
8.345 ± 0.35
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.569 ± 0.195
4.203 ± 0.297
3.678 ± 0.161
4.24 ± 0.285
5.558 ± 0.279
6.42 ± 0.378
5.806 ± 0.34
6.914 ± 0.339
1.423 ± 0.152
3.371 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here