Robbsia andropogonis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Robbsia

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4737 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F5JTQ6|A0A0F5JTQ6_9BURK Uncharacterized protein (Fragment) OS=Robbsia andropogonis OX=28092 GN=WM40_27485 PE=4 SV=1
MM1 pKa = 7.72ILDD4 pKa = 4.13GKK6 pKa = 10.38GGNDD10 pKa = 3.53TYY12 pKa = 11.27HH13 pKa = 6.96SYY15 pKa = 10.53YY16 pKa = 11.31ANDD19 pKa = 3.41TFVFNSGYY27 pKa = 10.38GALEE31 pKa = 3.97INNEE35 pKa = 4.09NYY37 pKa = 10.55SGGTFASVLRR47 pKa = 11.84FGPGVTLDD55 pKa = 3.78SLRR58 pKa = 11.84VTSDD62 pKa = 3.01GNVLVLRR69 pKa = 11.84DD70 pKa = 4.1GVDD73 pKa = 3.46GDD75 pKa = 4.54AVTVDD80 pKa = 3.6HH81 pKa = 6.75FFSEE85 pKa = 4.7FGWAGITSVEE95 pKa = 4.22LNDD98 pKa = 3.7GTVLNVSQLIQLEE111 pKa = 4.59EE112 pKa = 4.15TGSTGADD119 pKa = 2.98TLYY122 pKa = 9.58GTSGADD128 pKa = 3.42TIDD131 pKa = 3.38GRR133 pKa = 11.84GGNDD137 pKa = 3.22LAIGNGGADD146 pKa = 3.21TFFYY150 pKa = 10.45NAGYY154 pKa = 9.58GALEE158 pKa = 4.08ISSDD162 pKa = 3.7EE163 pKa = 4.27GSTPTSVLRR172 pKa = 11.84FGEE175 pKa = 4.99GITASSITVRR185 pKa = 11.84NSNSGTAIQITDD197 pKa = 4.84GIDD200 pKa = 3.34GDD202 pKa = 4.78VITIDD207 pKa = 4.1NMYY210 pKa = 10.3SDD212 pKa = 4.91SGTKK216 pKa = 10.36GVGSVEE222 pKa = 4.18FFDD225 pKa = 4.29GTTLTAQQLIALNAGRR241 pKa = 11.84APEE244 pKa = 3.8ATYY247 pKa = 11.12YY248 pKa = 9.2GTTGADD254 pKa = 3.57SITGSGEE261 pKa = 4.15DD262 pKa = 3.87EE263 pKa = 4.63LFDD266 pKa = 4.28GKK268 pKa = 11.24GGTDD272 pKa = 3.48YY273 pKa = 11.41FKK275 pKa = 11.58GNAGNDD281 pKa = 3.19TFVFNQGYY289 pKa = 8.53RR290 pKa = 11.84ALEE293 pKa = 3.89VDD295 pKa = 4.09EE296 pKa = 4.58NWYY299 pKa = 10.58SGQAPVLQLGAGIEE313 pKa = 3.97ASMLKK318 pKa = 10.48VSVANSHH325 pKa = 6.26SGLVITDD332 pKa = 3.9GVAGDD337 pKa = 5.14QITLDD342 pKa = 3.47NMLYY346 pKa = 10.25DD347 pKa = 3.95YY348 pKa = 11.2QGVSTIRR355 pKa = 11.84FADD358 pKa = 4.05GSTLSKK364 pKa = 10.7AQIIAMEE371 pKa = 4.31TTGTSGADD379 pKa = 3.06SMYY382 pKa = 10.84GSTAAEE388 pKa = 4.13LFDD391 pKa = 4.18GKK393 pKa = 11.04GGADD397 pKa = 3.49YY398 pKa = 11.41AKK400 pKa = 10.73GSGGNDD406 pKa = 2.49TFVFNEE412 pKa = 5.19GYY414 pKa = 9.88GQLEE418 pKa = 3.76IDD420 pKa = 3.46EE421 pKa = 4.65TLNDD425 pKa = 3.65GATTVLQLGAGITRR439 pKa = 11.84EE440 pKa = 3.96NIKK443 pKa = 10.7AYY445 pKa = 10.42FDD447 pKa = 3.72GATLVLTDD455 pKa = 5.65GISGDD460 pKa = 3.83QIRR463 pKa = 11.84IDD465 pKa = 3.45NEE467 pKa = 4.07KK468 pKa = 10.73YY469 pKa = 10.51SNNGINLVQFADD481 pKa = 4.21GATLTQADD489 pKa = 4.91LQTLPTTGSASNDD502 pKa = 3.42SLTGTGDD509 pKa = 3.49SEE511 pKa = 4.93VIDD514 pKa = 4.0GKK516 pKa = 11.22GGNDD520 pKa = 3.18TVNGNMGNDD529 pKa = 3.12TFVYY533 pKa = 8.58NQGYY537 pKa = 8.28GALEE541 pKa = 3.77INNNYY546 pKa = 9.55WYY548 pKa = 10.11GQNPVLQLGAGITAADD564 pKa = 3.91LQVATDD570 pKa = 3.84ASHH573 pKa = 6.81TGLILTDD580 pKa = 4.13GVAGDD585 pKa = 4.89TIHH588 pKa = 7.33IDD590 pKa = 3.6NIKK593 pKa = 8.51STEE596 pKa = 3.96RR597 pKa = 11.84TGVGSVTFADD607 pKa = 4.31GTTMSAADD615 pKa = 5.24LIALTTVGTTGNDD628 pKa = 3.19ALYY631 pKa = 10.49GSSSNDD637 pKa = 3.05MFDD640 pKa = 4.14GKK642 pKa = 11.1GGDD645 pKa = 3.72DD646 pKa = 4.03TITT649 pKa = 3.17

Molecular weight: 67.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F5JTU6|A0A0F5JTU6_9BURK Uncharacterized protein OS=Robbsia andropogonis OX=28092 GN=WM40_25385 PE=4 SV=1
MM1 pKa = 7.56AGWSLAWEE9 pKa = 4.43EE10 pKa = 5.34DD11 pKa = 3.51NLLHH15 pKa = 6.61CKK17 pKa = 10.35SFSHH21 pKa = 6.7CEE23 pKa = 3.54KK24 pKa = 10.33RR25 pKa = 11.84SRR27 pKa = 11.84NLKK30 pKa = 9.92IVVALSAGASYY41 pKa = 11.02NPHH44 pKa = 6.57RR45 pKa = 11.84LSAFRR50 pKa = 11.84AGARR54 pKa = 11.84VVRR57 pKa = 11.84IGMAYY62 pKa = 10.04AVASVCRR69 pKa = 11.84VAGSVLRR76 pKa = 11.84PVGRR80 pKa = 11.84DD81 pKa = 3.04AILRR85 pKa = 11.84RR86 pKa = 11.84QPCPQCQRR94 pKa = 11.84KK95 pKa = 8.69

Molecular weight: 10.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4737

0

4737

1470760

29

2373

310.5

33.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.419 ± 0.051

0.944 ± 0.01

5.683 ± 0.029

4.697 ± 0.03

3.598 ± 0.024

7.99 ± 0.031

2.419 ± 0.018

4.997 ± 0.027

3.027 ± 0.031

10.122 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.522 ± 0.016

2.845 ± 0.024

5.051 ± 0.027

3.653 ± 0.02

7.025 ± 0.039

5.96 ± 0.032

5.696 ± 0.029

7.565 ± 0.028

1.391 ± 0.013

2.396 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski