Elephant endotheliotropic herpesvirus 4
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 120 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S1TP62|A0A0S1TP62_9BETA Nuclear egress protein 2 OS=Elephant endotheliotropic herpesvirus 4 OX=548914 GN=U34 PE=3 SV=1
MM1 pKa = 7.41 KK2 pKa = 10.15 RR3 pKa = 11.84 YY4 pKa = 10.21 KK5 pKa = 10.4 NWAHH9 pKa = 6.78 ALNVHH14 pKa = 5.69 TASPAVTPEE23 pKa = 4.03 TFRR26 pKa = 11.84 LSYY29 pKa = 10.83 KK30 pKa = 10.59 LLILVAVFATTMEE43 pKa = 4.16 ISRR46 pKa = 11.84 EE47 pKa = 3.84 EE48 pKa = 4.27 DD49 pKa = 2.81 VDD51 pKa = 4.31 FCFEE55 pKa = 4.05 EE56 pKa = 4.91 VEE58 pKa = 4.26 QCEE61 pKa = 4.44 VEE63 pKa = 4.36 YY64 pKa = 11.05 ADD66 pKa = 4.78 PEE68 pKa = 4.62 DD69 pKa = 4.15 NEE71 pKa = 4.57 EE72 pKa = 4.05 VSDD75 pKa = 5.15 DD76 pKa = 4.39 DD77 pKa = 5.66 GEE79 pKa = 4.55 SSDD82 pKa = 4.57 GDD84 pKa = 4.12 SVDD87 pKa = 3.27 HH88 pKa = 7.32 AEE90 pKa = 4.04 EE91 pKa = 3.97 EE92 pKa = 4.13 KK93 pKa = 10.98 RR94 pKa = 11.84 MEE96 pKa = 4.16 EE97 pKa = 3.84 EE98 pKa = 3.6 CGYY101 pKa = 10.83 RR102 pKa = 11.84 DD103 pKa = 3.58 LPPRR107 pKa = 11.84 EE108 pKa = 4.09 VQGPPVCLDD117 pKa = 3.26 LTVIEE122 pKa = 5.23 PEE124 pKa = 4.02 MQTANIKK131 pKa = 9.9 CHH133 pKa = 5.39 EE134 pKa = 4.28 VCDD137 pKa = 4.38 EE138 pKa = 4.04 YY139 pKa = 11.7 GKK141 pKa = 10.44 RR142 pKa = 11.84 GYY144 pKa = 10.54 SFQGLIYY151 pKa = 10.08 YY152 pKa = 9.53 DD153 pKa = 3.84 PQEE156 pKa = 5.1 DD157 pKa = 4.93 DD158 pKa = 4.03 EE159 pKa = 7.44 DD160 pKa = 6.28 DD161 pKa = 4.22 DD162 pKa = 6.04 GEE164 pKa = 4.73 YY165 pKa = 10.79 DD166 pKa = 5.87 DD167 pKa = 6.04 EE168 pKa = 6.83 DD169 pKa = 4.52 FDD171 pKa = 4.75 EE172 pKa = 6.59 DD173 pKa = 4.3 EE174 pKa = 4.9 EE175 pKa = 4.56 EE176 pKa = 4.14 LTAMM180 pKa = 5.11
Molecular weight: 20.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.808
IPC_protein 3.795
Toseland 3.592
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.656
Rodwell 3.617
Grimsley 3.503
Solomon 3.757
Lehninger 3.706
Nozaki 3.872
DTASelect 4.037
Thurlkill 3.63
EMBOSS 3.668
Sillero 3.91
Patrickios 1.227
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|A0A0S1TQM3|A0A0S1TQM3_9BETA Large tegument protein OS=Elephant endotheliotropic herpesvirus 4 OX=548914 GN=U31 PE=3 SV=1
MM1 pKa = 6.63 QTTAMLLTICLGMALCGGGRR21 pKa = 11.84 RR22 pKa = 11.84 DD23 pKa = 3.43 GTVGRR28 pKa = 11.84 RR29 pKa = 11.84 TARR32 pKa = 11.84 DD33 pKa = 3.38 ASDD36 pKa = 4.69 LPIGDD41 pKa = 4.45 SPTSNKK47 pKa = 9.76 AARR50 pKa = 11.84 SPVRR54 pKa = 11.84 LPRR57 pKa = 11.84 NVSRR61 pKa = 11.84 VSGPVLLSPDD71 pKa = 4.22 SRR73 pKa = 11.84 PVMCGDD79 pKa = 4.42 VISKK83 pKa = 8.79 SICAYY88 pKa = 9.87 FVIDD92 pKa = 3.69 GVSRR96 pKa = 11.84 IKK98 pKa = 9.89 TRR100 pKa = 11.84 SFHH103 pKa = 6.48 HH104 pKa = 6.71 PNVLL108 pKa = 3.38
Molecular weight: 11.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.347
IPC2_protein 9.355
IPC_protein 10.087
Toseland 10.57
ProMoST 10.277
Dawson 10.643
Bjellqvist 10.394
Wikipedia 10.847
Rodwell 10.73
Grimsley 10.672
Solomon 10.804
Lehninger 10.774
Nozaki 10.628
DTASelect 10.365
Thurlkill 10.57
EMBOSS 10.965
Sillero 10.599
Patrickios 10.57
IPC_peptide 10.804
IPC2_peptide 9.897
IPC2.peptide.svr19 8.495
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
120
0
120
55876
79
2327
465.6
51.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.237 ± 0.235
2.33 ± 0.151
5.611 ± 0.185
4.701 ± 0.143
4.378 ± 0.204
6.432 ± 0.397
2.738 ± 0.102
4.594 ± 0.221
3.964 ± 0.177
9.075 ± 0.331
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.323 ± 0.111
4.494 ± 0.157
5.612 ± 0.328
3.34 ± 0.259
5.985 ± 0.185
8.046 ± 0.359
6.908 ± 0.269
7.318 ± 0.18
0.889 ± 0.071
4.023 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here