Upolu virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Insthoviricetes; Articulavirales; Orthomyxoviridae; Thogotovirus; unclassified Thogotovirus

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|X2CXC2|X2CXC2_9ORTO PA polymerase subunit OS=Upolu virus OX=1428581 GN=PA PE=4 SV=1
MM1 pKa = 7.78EE2 pKa = 6.01PPDD5 pKa = 5.32CIDD8 pKa = 3.09PRR10 pKa = 11.84IWEE13 pKa = 4.12KK14 pKa = 11.05SATQNDD20 pKa = 4.29WHH22 pKa = 7.99LMDD25 pKa = 4.04YY26 pKa = 10.55GYY28 pKa = 10.93EE29 pKa = 3.91RR30 pKa = 11.84RR31 pKa = 11.84IVACWEE37 pKa = 3.8YY38 pKa = 10.37TICCQISNMHH48 pKa = 6.53IFDD51 pKa = 4.09NMEE54 pKa = 3.68QYY56 pKa = 7.64EE57 pKa = 4.57TYY59 pKa = 10.38LWQDD63 pKa = 2.88RR64 pKa = 11.84ATVEE68 pKa = 3.71WLISKK73 pKa = 10.45RR74 pKa = 11.84GLQRR78 pKa = 11.84TTEE81 pKa = 4.47HH82 pKa = 6.36MPHH85 pKa = 6.23MYY87 pKa = 10.11DD88 pKa = 4.3RR89 pKa = 11.84KK90 pKa = 10.55NNLNFKK96 pKa = 10.05VKK98 pKa = 10.46VVMDD102 pKa = 4.4LEE104 pKa = 4.56EE105 pKa = 4.14VSPGGGVDD113 pKa = 2.59WCMAFDD119 pKa = 4.86LRR121 pKa = 11.84GEE123 pKa = 4.4THH125 pKa = 6.14QEE127 pKa = 3.89TYY129 pKa = 10.71QYY131 pKa = 11.55LLGSQLQDD139 pKa = 2.61IKK141 pKa = 11.5SYY143 pKa = 10.39MSSCYY148 pKa = 9.56EE149 pKa = 3.71WTIKK153 pKa = 9.79TGSTEE158 pKa = 4.27RR159 pKa = 11.84ILEE162 pKa = 4.25SMSSDD167 pKa = 2.99PKK169 pKa = 10.73VRR171 pKa = 11.84FVVPANVKK179 pKa = 10.34GLLTSDD185 pKa = 4.01TDD187 pKa = 3.31WDD189 pKa = 4.28IIPNTTPGKK198 pKa = 10.38GIAEE202 pKa = 3.99IVGEE206 pKa = 4.57KK207 pKa = 8.66WSQMKK212 pKa = 9.57VLPRR216 pKa = 11.84LKK218 pKa = 11.01YY219 pKa = 10.04EE220 pKa = 4.55DD221 pKa = 4.9PPNKK225 pKa = 10.34APWKK229 pKa = 9.46ALLIAADD236 pKa = 3.57SSYY239 pKa = 11.19IEE241 pKa = 4.52TKK243 pKa = 10.74NGTDD247 pKa = 3.43QEE249 pKa = 4.14ILGFFKK255 pKa = 10.71EE256 pKa = 4.53SIISVSTLRR265 pKa = 11.84SSKK268 pKa = 10.36SAPPYY273 pKa = 10.42LYY275 pKa = 10.38NCIKK279 pKa = 10.68ASVDD283 pKa = 3.29AAIEE287 pKa = 3.89GHH289 pKa = 5.16MVRR292 pKa = 11.84DD293 pKa = 4.59RR294 pKa = 11.84IVEE297 pKa = 3.95DD298 pKa = 3.59TKK300 pKa = 11.65AFGIDD305 pKa = 3.57FKK307 pKa = 11.43RR308 pKa = 11.84KK309 pKa = 8.5IRR311 pKa = 11.84NAAEE315 pKa = 3.77ATATGEE321 pKa = 4.31WKK323 pKa = 10.38RR324 pKa = 11.84SEE326 pKa = 4.28FPLEE330 pKa = 4.14KK331 pKa = 10.05QGLPSWVEE339 pKa = 3.92DD340 pKa = 4.11EE341 pKa = 5.49LDD343 pKa = 3.38LLYY346 pKa = 10.56RR347 pKa = 11.84EE348 pKa = 4.63CRR350 pKa = 11.84GRR352 pKa = 11.84WVEE355 pKa = 4.01LEE357 pKa = 4.39KK358 pKa = 10.88NAQYY362 pKa = 10.85TPIDD366 pKa = 4.02EE367 pKa = 4.41VAEE370 pKa = 4.16DD371 pKa = 4.4LVDD374 pKa = 3.85KK375 pKa = 10.71FIGLVDD381 pKa = 3.67TLKK384 pKa = 10.62VSSVIEE390 pKa = 3.98KK391 pKa = 9.04WQVGCTRR398 pKa = 11.84IFSQLHH404 pKa = 4.58TDD406 pKa = 3.48RR407 pKa = 11.84NRR409 pKa = 11.84ISLCPIITRR418 pKa = 11.84CKK420 pKa = 10.52EE421 pKa = 3.56EE422 pKa = 5.8DD423 pKa = 2.98IGQIWGLVLLGPHH436 pKa = 5.67HH437 pKa = 6.94VKK439 pKa = 10.42RR440 pKa = 11.84DD441 pKa = 3.44TDD443 pKa = 3.71NAPLLLFEE451 pKa = 4.87FVEE454 pKa = 4.28NCEE457 pKa = 3.89EE458 pKa = 3.92EE459 pKa = 5.1KK460 pKa = 11.02YY461 pKa = 10.34PKK463 pKa = 9.26HH464 pKa = 5.55TVLSLNMKK472 pKa = 10.11KK473 pKa = 10.32LDD475 pKa = 3.5GEE477 pKa = 4.69IYY479 pKa = 10.04TKK481 pKa = 10.76LAAIKK486 pKa = 9.08VTSCSRR492 pKa = 11.84NKK494 pKa = 10.5LFTFSTIRR502 pKa = 11.84RR503 pKa = 11.84VLIQPASVFSQILLQRR519 pKa = 11.84AADD522 pKa = 3.92NNEE525 pKa = 4.19LNLDD529 pKa = 4.01SNPEE533 pKa = 3.54VSLYY537 pKa = 10.42IGGALRR543 pKa = 11.84GMTAFQWVRR552 pKa = 11.84KK553 pKa = 7.53VLCLEE558 pKa = 4.03FLMAIYY564 pKa = 10.94NNAQLEE570 pKa = 4.4GFLANIRR577 pKa = 11.84RR578 pKa = 11.84LHH580 pKa = 5.4MSRR583 pKa = 11.84HH584 pKa = 4.67AMIEE588 pKa = 3.83RR589 pKa = 11.84HH590 pKa = 5.13QVFIPFGSDD599 pKa = 2.78PASKK603 pKa = 10.78VEE605 pKa = 3.81EE606 pKa = 4.58CIINNPIVLYY616 pKa = 10.19LAKK619 pKa = 9.57TWNDD623 pKa = 3.4MPNVHH628 pKa = 6.27YY629 pKa = 10.83

Molecular weight:
72.49 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|X2CUE6|X2CUE6_9ORTO Matrix OS=Upolu virus OX=1428581 GN=ML PE=4 SV=1
MM1 pKa = 7.49EE2 pKa = 5.72SEE4 pKa = 4.17PQEE7 pKa = 3.75QLKK10 pKa = 10.14FLNHH14 pKa = 7.57LITKK18 pKa = 9.36FKK20 pKa = 11.05GCLAEE25 pKa = 6.03AKK27 pKa = 8.08DD28 pKa = 3.75TLQKK32 pKa = 11.01KK33 pKa = 6.71FTEE36 pKa = 4.25YY37 pKa = 10.91EE38 pKa = 3.86VFKK41 pKa = 10.75RR42 pKa = 11.84YY43 pKa = 6.49TTSKK47 pKa = 10.22KK48 pKa = 10.45DD49 pKa = 3.23HH50 pKa = 6.56APQMRR55 pKa = 11.84LCYY58 pKa = 10.05SIRR61 pKa = 11.84KK62 pKa = 7.39PLPIIMKK69 pKa = 6.21PTKK72 pKa = 10.15KK73 pKa = 10.08IPEE76 pKa = 4.33VYY78 pKa = 10.33NGTVLKK84 pKa = 10.07EE85 pKa = 3.88LKK87 pKa = 10.02YY88 pKa = 11.03DD89 pKa = 3.55LGEE92 pKa = 4.09SSKK95 pKa = 10.24IRR97 pKa = 11.84CSVLVPDD104 pKa = 4.04YY105 pKa = 9.4WSKK108 pKa = 11.3YY109 pKa = 10.27GEE111 pKa = 4.28TTTLEE116 pKa = 3.71IADD119 pKa = 4.85AITYY123 pKa = 7.2TEE125 pKa = 3.95RR126 pKa = 11.84LKK128 pKa = 11.08VEE130 pKa = 4.21RR131 pKa = 11.84FFSAEE136 pKa = 3.2WGEE139 pKa = 3.84IRR141 pKa = 11.84FGKK144 pKa = 8.5MLPYY148 pKa = 10.29RR149 pKa = 11.84KK150 pKa = 8.84PVQANPTIVDD160 pKa = 3.53MSSSNVSHH168 pKa = 6.28TLIQVFCPQYY178 pKa = 10.36ATLDD182 pKa = 3.82SKK184 pKa = 11.28RR185 pKa = 11.84KK186 pKa = 8.85ANQEE190 pKa = 3.73AVEE193 pKa = 4.03KK194 pKa = 9.79LKK196 pKa = 11.3VVMSPITGYY205 pKa = 8.53RR206 pKa = 11.84TQEE209 pKa = 3.77AAIHH213 pKa = 6.22IARR216 pKa = 11.84SLIQSEE222 pKa = 4.81KK223 pKa = 9.57KK224 pKa = 9.15WLPTVVDD231 pKa = 4.21HH232 pKa = 6.75TPHH235 pKa = 5.59TAEE238 pKa = 4.28MAHH241 pKa = 6.18FLCSRR246 pKa = 11.84YY247 pKa = 9.9HH248 pKa = 6.43YY249 pKa = 10.39LATSNRR255 pKa = 11.84EE256 pKa = 3.9PNTTKK261 pKa = 11.0SMDD264 pKa = 3.88SLCGEE269 pKa = 4.5LVRR272 pKa = 11.84RR273 pKa = 11.84SLKK276 pKa = 9.32TRR278 pKa = 11.84APKK281 pKa = 8.94EE282 pKa = 3.85TLQANLSKK290 pKa = 11.1VKK292 pKa = 10.33IQGRR296 pKa = 11.84PLSEE300 pKa = 3.8VLKK303 pKa = 10.11DD304 pKa = 3.7HH305 pKa = 6.54EE306 pKa = 4.81GEE308 pKa = 4.07YY309 pKa = 10.06PYY311 pKa = 11.18LGICRR316 pKa = 11.84VALGLTTHH324 pKa = 6.15YY325 pKa = 10.78SILIRR330 pKa = 11.84NTKK333 pKa = 9.05FVIMSSDD340 pKa = 3.85LPRR343 pKa = 11.84KK344 pKa = 5.65EE345 pKa = 4.09TKK347 pKa = 10.22KK348 pKa = 10.3IFEE351 pKa = 4.89LSPEE355 pKa = 4.11VKK357 pKa = 9.9TIIPYY362 pKa = 9.58SHH364 pKa = 6.61FQGFARR370 pKa = 11.84VYY372 pKa = 7.93FQAGAVHH379 pKa = 7.24GYY381 pKa = 7.5FTSTDD386 pKa = 3.4RR387 pKa = 11.84ALHH390 pKa = 6.24EE391 pKa = 4.23IHH393 pKa = 6.6LTAPDD398 pKa = 4.01NAPLMDD404 pKa = 5.88IILDD408 pKa = 3.45ICYY411 pKa = 9.24YY412 pKa = 9.78GAYY415 pKa = 9.22IEE417 pKa = 5.09PGFEE421 pKa = 3.94YY422 pKa = 10.46TFGYY426 pKa = 10.04FPSNKK431 pKa = 8.84RR432 pKa = 11.84DD433 pKa = 3.43FFDD436 pKa = 4.45NFFLHH441 pKa = 6.6HH442 pKa = 6.83SKK444 pKa = 10.75DD445 pKa = 3.08HH446 pKa = 6.09RR447 pKa = 11.84AFLKK451 pKa = 10.89VMGIEE456 pKa = 3.97EE457 pKa = 4.69TKK459 pKa = 10.43PGLYY463 pKa = 10.39LSPEE467 pKa = 4.38LNWKK471 pKa = 9.66EE472 pKa = 3.79EE473 pKa = 4.03SRR475 pKa = 11.84GKK477 pKa = 10.26VSRR480 pKa = 11.84VYY482 pKa = 10.09EE483 pKa = 4.16LSTIVQPYY491 pKa = 7.97NQEE494 pKa = 3.61EE495 pKa = 4.41KK496 pKa = 10.74KK497 pKa = 10.69FVIGEE502 pKa = 4.18TLSVYY507 pKa = 10.21VEE509 pKa = 4.09HH510 pKa = 7.16EE511 pKa = 4.29RR512 pKa = 11.84GLEE515 pKa = 3.77LLINPSEE522 pKa = 4.17IKK524 pKa = 10.29HH525 pKa = 5.2SQRR528 pKa = 11.84HH529 pKa = 5.24PLPIGLDD536 pKa = 3.78LSQSEE541 pKa = 5.0FIDD544 pKa = 3.53IYY546 pKa = 10.02MDD548 pKa = 4.23PYY550 pKa = 11.43SRR552 pKa = 11.84AKK554 pKa = 10.74NLFKK558 pKa = 10.89SITLNKK564 pKa = 8.95EE565 pKa = 3.38RR566 pKa = 11.84CKK568 pKa = 10.89EE569 pKa = 3.83FVGNMLEE576 pKa = 4.13EE577 pKa = 4.47HH578 pKa = 6.41QDD580 pKa = 3.39PAEE583 pKa = 4.22GVVQDD588 pKa = 4.6LVPVNTWARR597 pKa = 11.84SAKK600 pKa = 10.02RR601 pKa = 11.84KK602 pKa = 8.1IHH604 pKa = 4.96EE605 pKa = 4.94TIDD608 pKa = 3.93EE609 pKa = 4.3DD610 pKa = 4.19EE611 pKa = 4.19QWYY614 pKa = 8.9EE615 pKa = 4.14CPKK618 pKa = 10.54KK619 pKa = 10.05RR620 pKa = 11.84IKK622 pKa = 9.74MGYY625 pKa = 8.15FALIAGSLQHH635 pKa = 6.75RR636 pKa = 11.84SKK638 pKa = 10.83KK639 pKa = 9.72PSVVPKK645 pKa = 10.55EE646 pKa = 3.87FVLNNTQVVLDD657 pKa = 3.99SRR659 pKa = 11.84CSKK662 pKa = 10.94GLIVDD667 pKa = 3.95TGNRR671 pKa = 11.84VIIGGEE677 pKa = 3.92TILRR681 pKa = 11.84NEE683 pKa = 4.23VKK685 pKa = 10.65HH686 pKa = 6.07LGGYY690 pKa = 8.38IQTGVFDD697 pKa = 4.71HH698 pKa = 6.81QPQCYY703 pKa = 9.45LVDD706 pKa = 5.39DD707 pKa = 4.62IEE709 pKa = 5.45SGQQMEE715 pKa = 4.73LSRR718 pKa = 11.84FCIQSQGRR726 pKa = 11.84FFQYY730 pKa = 9.76EE731 pKa = 4.05KK732 pKa = 10.6KK733 pKa = 10.01ISVWEE738 pKa = 3.88EE739 pKa = 3.34TDD741 pKa = 3.53NIKK744 pKa = 9.6ATLEE748 pKa = 4.11GQKK751 pKa = 9.2SQKK754 pKa = 10.07RR755 pKa = 11.84RR756 pKa = 11.84MEE758 pKa = 3.97AEE760 pKa = 3.58ARR762 pKa = 11.84IEE764 pKa = 4.2SLVKK768 pKa = 10.23RR769 pKa = 11.84PRR771 pKa = 11.84RR772 pKa = 11.84DD773 pKa = 2.99VV774 pKa = 3.11

Molecular weight:
89.41 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3384

271

774

564.0

64.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.792 ± 0.428

2.009 ± 0.322

4.905 ± 0.264

7.21 ± 0.512

4.019 ± 0.234

5.526 ± 0.386

2.512 ± 0.26

5.999 ± 0.352

7.004 ± 0.407

9.338 ± 0.407

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.926 ± 0.377

4.167 ± 0.375

4.61 ± 0.176

3.871 ± 0.213

5.378 ± 0.17

7.358 ± 0.478

5.999 ± 0.185

6.087 ± 0.131

1.596 ± 0.295

3.694 ± 0.25

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski