Upolu virus
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|X2CXC2|X2CXC2_9ORTO PA polymerase subunit OS=Upolu virus OX=1428581 GN=PA PE=4 SV=1
MM1 pKa = 7.78 EE2 pKa = 6.01 PPDD5 pKa = 5.32 CIDD8 pKa = 3.09 PRR10 pKa = 11.84 IWEE13 pKa = 4.12 KK14 pKa = 11.05 SATQNDD20 pKa = 4.29 WHH22 pKa = 7.99 LMDD25 pKa = 4.04 YY26 pKa = 10.55 GYY28 pKa = 10.93 EE29 pKa = 3.91 RR30 pKa = 11.84 RR31 pKa = 11.84 IVACWEE37 pKa = 3.8 YY38 pKa = 10.37 TICCQISNMHH48 pKa = 6.53 IFDD51 pKa = 4.09 NMEE54 pKa = 3.68 QYY56 pKa = 7.64 EE57 pKa = 4.57 TYY59 pKa = 10.38 LWQDD63 pKa = 2.88 RR64 pKa = 11.84 ATVEE68 pKa = 3.71 WLISKK73 pKa = 10.45 RR74 pKa = 11.84 GLQRR78 pKa = 11.84 TTEE81 pKa = 4.47 HH82 pKa = 6.36 MPHH85 pKa = 6.23 MYY87 pKa = 10.11 DD88 pKa = 4.3 RR89 pKa = 11.84 KK90 pKa = 10.55 NNLNFKK96 pKa = 10.05 VKK98 pKa = 10.46 VVMDD102 pKa = 4.4 LEE104 pKa = 4.56 EE105 pKa = 4.14 VSPGGGVDD113 pKa = 2.59 WCMAFDD119 pKa = 4.86 LRR121 pKa = 11.84 GEE123 pKa = 4.4 THH125 pKa = 6.14 QEE127 pKa = 3.89 TYY129 pKa = 10.71 QYY131 pKa = 11.55 LLGSQLQDD139 pKa = 2.61 IKK141 pKa = 11.5 SYY143 pKa = 10.39 MSSCYY148 pKa = 9.56 EE149 pKa = 3.71 WTIKK153 pKa = 9.79 TGSTEE158 pKa = 4.27 RR159 pKa = 11.84 ILEE162 pKa = 4.25 SMSSDD167 pKa = 2.99 PKK169 pKa = 10.73 VRR171 pKa = 11.84 FVVPANVKK179 pKa = 10.34 GLLTSDD185 pKa = 4.01 TDD187 pKa = 3.31 WDD189 pKa = 4.28 IIPNTTPGKK198 pKa = 10.38 GIAEE202 pKa = 3.99 IVGEE206 pKa = 4.57 KK207 pKa = 8.66 WSQMKK212 pKa = 9.57 VLPRR216 pKa = 11.84 LKK218 pKa = 11.01 YY219 pKa = 10.04 EE220 pKa = 4.55 DD221 pKa = 4.9 PPNKK225 pKa = 10.34 APWKK229 pKa = 9.46 ALLIAADD236 pKa = 3.57 SSYY239 pKa = 11.19 IEE241 pKa = 4.52 TKK243 pKa = 10.74 NGTDD247 pKa = 3.43 QEE249 pKa = 4.14 ILGFFKK255 pKa = 10.71 EE256 pKa = 4.53 SIISVSTLRR265 pKa = 11.84 SSKK268 pKa = 10.36 SAPPYY273 pKa = 10.42 LYY275 pKa = 10.38 NCIKK279 pKa = 10.68 ASVDD283 pKa = 3.29 AAIEE287 pKa = 3.89 GHH289 pKa = 5.16 MVRR292 pKa = 11.84 DD293 pKa = 4.59 RR294 pKa = 11.84 IVEE297 pKa = 3.95 DD298 pKa = 3.59 TKK300 pKa = 11.65 AFGIDD305 pKa = 3.57 FKK307 pKa = 11.43 RR308 pKa = 11.84 KK309 pKa = 8.5 IRR311 pKa = 11.84 NAAEE315 pKa = 3.77 ATATGEE321 pKa = 4.31 WKK323 pKa = 10.38 RR324 pKa = 11.84 SEE326 pKa = 4.28 FPLEE330 pKa = 4.14 KK331 pKa = 10.05 QGLPSWVEE339 pKa = 3.92 DD340 pKa = 4.11 EE341 pKa = 5.49 LDD343 pKa = 3.38 LLYY346 pKa = 10.56 RR347 pKa = 11.84 EE348 pKa = 4.63 CRR350 pKa = 11.84 GRR352 pKa = 11.84 WVEE355 pKa = 4.01 LEE357 pKa = 4.39 KK358 pKa = 10.88 NAQYY362 pKa = 10.85 TPIDD366 pKa = 4.02 EE367 pKa = 4.41 VAEE370 pKa = 4.16 DD371 pKa = 4.4 LVDD374 pKa = 3.85 KK375 pKa = 10.71 FIGLVDD381 pKa = 3.67 TLKK384 pKa = 10.62 VSSVIEE390 pKa = 3.98 KK391 pKa = 9.04 WQVGCTRR398 pKa = 11.84 IFSQLHH404 pKa = 4.58 TDD406 pKa = 3.48 RR407 pKa = 11.84 NRR409 pKa = 11.84 ISLCPIITRR418 pKa = 11.84 CKK420 pKa = 10.52 EE421 pKa = 3.56 EE422 pKa = 5.8 DD423 pKa = 2.98 IGQIWGLVLLGPHH436 pKa = 5.67 HH437 pKa = 6.94 VKK439 pKa = 10.42 RR440 pKa = 11.84 DD441 pKa = 3.44 TDD443 pKa = 3.71 NAPLLLFEE451 pKa = 4.87 FVEE454 pKa = 4.28 NCEE457 pKa = 3.89 EE458 pKa = 3.92 EE459 pKa = 5.1 KK460 pKa = 11.02 YY461 pKa = 10.34 PKK463 pKa = 9.26 HH464 pKa = 5.55 TVLSLNMKK472 pKa = 10.11 KK473 pKa = 10.32 LDD475 pKa = 3.5 GEE477 pKa = 4.69 IYY479 pKa = 10.04 TKK481 pKa = 10.76 LAAIKK486 pKa = 9.08 VTSCSRR492 pKa = 11.84 NKK494 pKa = 10.5 LFTFSTIRR502 pKa = 11.84 RR503 pKa = 11.84 VLIQPASVFSQILLQRR519 pKa = 11.84 AADD522 pKa = 3.92 NNEE525 pKa = 4.19 LNLDD529 pKa = 4.01 SNPEE533 pKa = 3.54 VSLYY537 pKa = 10.42 IGGALRR543 pKa = 11.84 GMTAFQWVRR552 pKa = 11.84 KK553 pKa = 7.53 VLCLEE558 pKa = 4.03 FLMAIYY564 pKa = 10.94 NNAQLEE570 pKa = 4.4 GFLANIRR577 pKa = 11.84 RR578 pKa = 11.84 LHH580 pKa = 5.4 MSRR583 pKa = 11.84 HH584 pKa = 4.67 AMIEE588 pKa = 3.83 RR589 pKa = 11.84 HH590 pKa = 5.13 QVFIPFGSDD599 pKa = 2.78 PASKK603 pKa = 10.78 VEE605 pKa = 3.81 EE606 pKa = 4.58 CIINNPIVLYY616 pKa = 10.19 LAKK619 pKa = 9.57 TWNDD623 pKa = 3.4 MPNVHH628 pKa = 6.27 YY629 pKa = 10.83
Molecular weight: 72.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.49
IPC2_protein 5.525
IPC_protein 5.537
Toseland 5.741
ProMoST 5.792
Dawson 5.677
Bjellqvist 5.741
Wikipedia 5.639
Rodwell 5.652
Grimsley 5.817
Solomon 5.677
Lehninger 5.664
Nozaki 5.893
DTASelect 6.071
Thurlkill 5.982
EMBOSS 5.931
Sillero 5.995
Patrickios 4.101
IPC_peptide 5.703
IPC2_peptide 5.982
IPC2.peptide.svr19 6.025
Protein with the highest isoelectric point:
>tr|X2CUE6|X2CUE6_9ORTO Matrix OS=Upolu virus OX=1428581 GN=ML PE=4 SV=1
MM1 pKa = 7.49 EE2 pKa = 5.72 SEE4 pKa = 4.17 PQEE7 pKa = 3.75 QLKK10 pKa = 10.14 FLNHH14 pKa = 7.57 LITKK18 pKa = 9.36 FKK20 pKa = 11.05 GCLAEE25 pKa = 6.03 AKK27 pKa = 8.08 DD28 pKa = 3.75 TLQKK32 pKa = 11.01 KK33 pKa = 6.71 FTEE36 pKa = 4.25 YY37 pKa = 10.91 EE38 pKa = 3.86 VFKK41 pKa = 10.75 RR42 pKa = 11.84 YY43 pKa = 6.49 TTSKK47 pKa = 10.22 KK48 pKa = 10.45 DD49 pKa = 3.23 HH50 pKa = 6.56 APQMRR55 pKa = 11.84 LCYY58 pKa = 10.05 SIRR61 pKa = 11.84 KK62 pKa = 7.39 PLPIIMKK69 pKa = 6.21 PTKK72 pKa = 10.15 KK73 pKa = 10.08 IPEE76 pKa = 4.33 VYY78 pKa = 10.33 NGTVLKK84 pKa = 10.07 EE85 pKa = 3.88 LKK87 pKa = 10.02 YY88 pKa = 11.03 DD89 pKa = 3.55 LGEE92 pKa = 4.09 SSKK95 pKa = 10.24 IRR97 pKa = 11.84 CSVLVPDD104 pKa = 4.04 YY105 pKa = 9.4 WSKK108 pKa = 11.3 YY109 pKa = 10.27 GEE111 pKa = 4.28 TTTLEE116 pKa = 3.71 IADD119 pKa = 4.85 AITYY123 pKa = 7.2 TEE125 pKa = 3.95 RR126 pKa = 11.84 LKK128 pKa = 11.08 VEE130 pKa = 4.21 RR131 pKa = 11.84 FFSAEE136 pKa = 3.2 WGEE139 pKa = 3.84 IRR141 pKa = 11.84 FGKK144 pKa = 8.5 MLPYY148 pKa = 10.29 RR149 pKa = 11.84 KK150 pKa = 8.84 PVQANPTIVDD160 pKa = 3.53 MSSSNVSHH168 pKa = 6.28 TLIQVFCPQYY178 pKa = 10.36 ATLDD182 pKa = 3.82 SKK184 pKa = 11.28 RR185 pKa = 11.84 KK186 pKa = 8.85 ANQEE190 pKa = 3.73 AVEE193 pKa = 4.03 KK194 pKa = 9.79 LKK196 pKa = 11.3 VVMSPITGYY205 pKa = 8.53 RR206 pKa = 11.84 TQEE209 pKa = 3.77 AAIHH213 pKa = 6.22 IARR216 pKa = 11.84 SLIQSEE222 pKa = 4.81 KK223 pKa = 9.57 KK224 pKa = 9.15 WLPTVVDD231 pKa = 4.21 HH232 pKa = 6.75 TPHH235 pKa = 5.59 TAEE238 pKa = 4.28 MAHH241 pKa = 6.18 FLCSRR246 pKa = 11.84 YY247 pKa = 9.9 HH248 pKa = 6.43 YY249 pKa = 10.39 LATSNRR255 pKa = 11.84 EE256 pKa = 3.9 PNTTKK261 pKa = 11.0 SMDD264 pKa = 3.88 SLCGEE269 pKa = 4.5 LVRR272 pKa = 11.84 RR273 pKa = 11.84 SLKK276 pKa = 9.32 TRR278 pKa = 11.84 APKK281 pKa = 8.94 EE282 pKa = 3.85 TLQANLSKK290 pKa = 11.1 VKK292 pKa = 10.33 IQGRR296 pKa = 11.84 PLSEE300 pKa = 3.8 VLKK303 pKa = 10.11 DD304 pKa = 3.7 HH305 pKa = 6.54 EE306 pKa = 4.81 GEE308 pKa = 4.07 YY309 pKa = 10.06 PYY311 pKa = 11.18 LGICRR316 pKa = 11.84 VALGLTTHH324 pKa = 6.15 YY325 pKa = 10.78 SILIRR330 pKa = 11.84 NTKK333 pKa = 9.05 FVIMSSDD340 pKa = 3.85 LPRR343 pKa = 11.84 KK344 pKa = 5.65 EE345 pKa = 4.09 TKK347 pKa = 10.22 KK348 pKa = 10.3 IFEE351 pKa = 4.89 LSPEE355 pKa = 4.11 VKK357 pKa = 9.9 TIIPYY362 pKa = 9.58 SHH364 pKa = 6.61 FQGFARR370 pKa = 11.84 VYY372 pKa = 7.93 FQAGAVHH379 pKa = 7.24 GYY381 pKa = 7.5 FTSTDD386 pKa = 3.4 RR387 pKa = 11.84 ALHH390 pKa = 6.24 EE391 pKa = 4.23 IHH393 pKa = 6.6 LTAPDD398 pKa = 4.01 NAPLMDD404 pKa = 5.88 IILDD408 pKa = 3.45 ICYY411 pKa = 9.24 YY412 pKa = 9.78 GAYY415 pKa = 9.22 IEE417 pKa = 5.09 PGFEE421 pKa = 3.94 YY422 pKa = 10.46 TFGYY426 pKa = 10.04 FPSNKK431 pKa = 8.84 RR432 pKa = 11.84 DD433 pKa = 3.43 FFDD436 pKa = 4.45 NFFLHH441 pKa = 6.6 HH442 pKa = 6.83 SKK444 pKa = 10.75 DD445 pKa = 3.08 HH446 pKa = 6.09 RR447 pKa = 11.84 AFLKK451 pKa = 10.89 VMGIEE456 pKa = 3.97 EE457 pKa = 4.69 TKK459 pKa = 10.43 PGLYY463 pKa = 10.39 LSPEE467 pKa = 4.38 LNWKK471 pKa = 9.66 EE472 pKa = 3.79 EE473 pKa = 4.03 SRR475 pKa = 11.84 GKK477 pKa = 10.26 VSRR480 pKa = 11.84 VYY482 pKa = 10.09 EE483 pKa = 4.16 LSTIVQPYY491 pKa = 7.97 NQEE494 pKa = 3.61 EE495 pKa = 4.41 KK496 pKa = 10.74 KK497 pKa = 10.69 FVIGEE502 pKa = 4.18 TLSVYY507 pKa = 10.21 VEE509 pKa = 4.09 HH510 pKa = 7.16 EE511 pKa = 4.29 RR512 pKa = 11.84 GLEE515 pKa = 3.77 LLINPSEE522 pKa = 4.17 IKK524 pKa = 10.29 HH525 pKa = 5.2 SQRR528 pKa = 11.84 HH529 pKa = 5.24 PLPIGLDD536 pKa = 3.78 LSQSEE541 pKa = 5.0 FIDD544 pKa = 3.53 IYY546 pKa = 10.02 MDD548 pKa = 4.23 PYY550 pKa = 11.43 SRR552 pKa = 11.84 AKK554 pKa = 10.74 NLFKK558 pKa = 10.89 SITLNKK564 pKa = 8.95 EE565 pKa = 3.38 RR566 pKa = 11.84 CKK568 pKa = 10.89 EE569 pKa = 3.83 FVGNMLEE576 pKa = 4.13 EE577 pKa = 4.47 HH578 pKa = 6.41 QDD580 pKa = 3.39 PAEE583 pKa = 4.22 GVVQDD588 pKa = 4.6 LVPVNTWARR597 pKa = 11.84 SAKK600 pKa = 10.02 RR601 pKa = 11.84 KK602 pKa = 8.1 IHH604 pKa = 4.96 EE605 pKa = 4.94 TIDD608 pKa = 3.93 EE609 pKa = 4.3 DD610 pKa = 4.19 EE611 pKa = 4.19 QWYY614 pKa = 8.9 EE615 pKa = 4.14 CPKK618 pKa = 10.54 KK619 pKa = 10.05 RR620 pKa = 11.84 IKK622 pKa = 9.74 MGYY625 pKa = 8.15 FALIAGSLQHH635 pKa = 6.75 RR636 pKa = 11.84 SKK638 pKa = 10.83 KK639 pKa = 9.72 PSVVPKK645 pKa = 10.55 EE646 pKa = 3.87 FVLNNTQVVLDD657 pKa = 3.99 SRR659 pKa = 11.84 CSKK662 pKa = 10.94 GLIVDD667 pKa = 3.95 TGNRR671 pKa = 11.84 VIIGGEE677 pKa = 3.92 TILRR681 pKa = 11.84 NEE683 pKa = 4.23 VKK685 pKa = 10.65 HH686 pKa = 6.07 LGGYY690 pKa = 8.38 IQTGVFDD697 pKa = 4.71 HH698 pKa = 6.81 QPQCYY703 pKa = 9.45 LVDD706 pKa = 5.39 DD707 pKa = 4.62 IEE709 pKa = 5.45 SGQQMEE715 pKa = 4.73 LSRR718 pKa = 11.84 FCIQSQGRR726 pKa = 11.84 FFQYY730 pKa = 9.76 EE731 pKa = 4.05 KK732 pKa = 10.6 KK733 pKa = 10.01 ISVWEE738 pKa = 3.88 EE739 pKa = 3.34 TDD741 pKa = 3.53 NIKK744 pKa = 9.6 ATLEE748 pKa = 4.11 GQKK751 pKa = 9.2 SQKK754 pKa = 10.07 RR755 pKa = 11.84 RR756 pKa = 11.84 MEE758 pKa = 3.97 AEE760 pKa = 3.58 ARR762 pKa = 11.84 IEE764 pKa = 4.2 SLVKK768 pKa = 10.23 RR769 pKa = 11.84 PRR771 pKa = 11.84 RR772 pKa = 11.84 DD773 pKa = 2.99 VV774 pKa = 3.11
Molecular weight: 89.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.732
IPC2_protein 7.995
IPC_protein 7.819
Toseland 7.863
ProMoST 8.404
Dawson 8.668
Bjellqvist 8.799
Wikipedia 8.682
Rodwell 8.756
Grimsley 7.936
Solomon 8.726
Lehninger 8.712
Nozaki 8.931
DTASelect 8.609
Thurlkill 8.697
EMBOSS 8.843
Sillero 8.946
Patrickios 4.444
IPC_peptide 8.712
IPC2_peptide 7.541
IPC2.peptide.svr19 7.78
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3384
271
774
564.0
64.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.792 ± 0.428
2.009 ± 0.322
4.905 ± 0.264
7.21 ± 0.512
4.019 ± 0.234
5.526 ± 0.386
2.512 ± 0.26
5.999 ± 0.352
7.004 ± 0.407
9.338 ± 0.407
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.926 ± 0.377
4.167 ± 0.375
4.61 ± 0.176
3.871 ± 0.213
5.378 ± 0.17
7.358 ± 0.478
5.999 ± 0.185
6.087 ± 0.131
1.596 ± 0.295
3.694 ± 0.25
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here