Bacillus phage AR9

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 291 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A172JIA0|A0A172JIA0_9CAUD Uncharacterized protein OS=Bacillus phage AR9 OX=1815509 GN=AR9_g192 PE=4 SV=1
MM1 pKa = 7.44TDD3 pKa = 2.73IYY5 pKa = 11.35YY6 pKa = 11.04KK7 pKa = 10.38LLYY10 pKa = 9.89YY11 pKa = 10.07ISEE14 pKa = 4.05NGKK17 pKa = 9.52PKK19 pKa = 9.71VTVATLQWFDD29 pKa = 4.37EE30 pKa = 4.52LDD32 pKa = 4.43DD33 pKa = 3.9NDD35 pKa = 5.45KK36 pKa = 10.98YY37 pKa = 11.62LKK39 pKa = 11.0DD40 pKa = 3.81EE41 pKa = 5.57DD42 pKa = 5.01GDD44 pKa = 4.56DD45 pKa = 3.42LLFYY49 pKa = 10.98NEE51 pKa = 4.18EE52 pKa = 4.13EE53 pKa = 4.14AVQWLLDD60 pKa = 3.54NFQEE64 pKa = 4.31NEE66 pKa = 3.71IDD68 pKa = 4.46SEE70 pKa = 4.57YY71 pKa = 11.09LNKK74 pKa = 10.56

Molecular weight: 8.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A172JI69|A0A172JI69_9CAUD Uncharacterized protein OS=Bacillus phage AR9 OX=1815509 GN=AR9_g162 PE=4 SV=1
MM1 pKa = 6.66NTYY4 pKa = 10.6YY5 pKa = 10.77DD6 pKa = 3.95YY7 pKa = 11.94SNQTKK12 pKa = 8.27KK13 pKa = 8.55TLKK16 pKa = 9.01TNEE19 pKa = 3.78RR20 pKa = 11.84SLSRR24 pKa = 11.84KK25 pKa = 7.26EE26 pKa = 3.72RR27 pKa = 11.84RR28 pKa = 11.84EE29 pKa = 3.69LAKK32 pKa = 10.73LEE34 pKa = 3.9EE35 pKa = 4.34RR36 pKa = 11.84EE37 pKa = 3.84RR38 pKa = 11.84RR39 pKa = 11.84LYY41 pKa = 10.54DD42 pKa = 3.19KK43 pKa = 10.58KK44 pKa = 10.72QRR46 pKa = 11.84EE47 pKa = 3.77IAKK50 pKa = 10.03RR51 pKa = 11.84LGFIKK56 pKa = 10.6

Molecular weight: 7.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

291

0

291

75221

39

1925

258.5

29.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.568 ± 0.173

0.742 ± 0.067

6.84 ± 0.135

7.728 ± 0.17

4.648 ± 0.108

4.648 ± 0.22

1.424 ± 0.061

9.463 ± 0.165

9.755 ± 0.169

8.641 ± 0.166

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.232 ± 0.074

8.258 ± 0.133

2.507 ± 0.094

2.564 ± 0.065

3.676 ± 0.086

7.019 ± 0.154

5.451 ± 0.152

5.191 ± 0.104

0.698 ± 0.048

4.947 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski