Dragonfly circularisvirus
Average proteome isoelectric point is 8.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K0A2Q4|K0A2Q4_9VIRU Replication-associated protein OS=Dragonfly circularisvirus OX=1234872 PE=4 SV=1
MM1 pKa = 7.32 SRR3 pKa = 11.84 CKK5 pKa = 10.06 NWCFTSFNVDD15 pKa = 3.43 SPPKK19 pKa = 9.57 FDD21 pKa = 6.43 PITTEE26 pKa = 3.74 YY27 pKa = 10.66 LCYY30 pKa = 10.48 GRR32 pKa = 11.84 EE33 pKa = 4.03 TCPTTKK39 pKa = 9.99 KK40 pKa = 10.25 SHH42 pKa = 5.73 LQGFICLKK50 pKa = 8.64 EE51 pKa = 4.34 RR52 pKa = 11.84 KK53 pKa = 9.25 RR54 pKa = 11.84 LAGVKK59 pKa = 9.79 KK60 pKa = 10.64 LLGSVHH66 pKa = 7.07 LEE68 pKa = 3.87 ASRR71 pKa = 11.84 GTVDD75 pKa = 5.28 QNIQYY80 pKa = 9.95 CSKK83 pKa = 10.72 DD84 pKa = 3.37 GEE86 pKa = 4.3 FTEE89 pKa = 5.21 FGSRR93 pKa = 11.84 PSSGPATSPFALAIAAATDD112 pKa = 3.24 GRR114 pKa = 11.84 LDD116 pKa = 3.97 DD117 pKa = 4.6 VKK119 pKa = 10.42 STHH122 pKa = 6.33 PGIYY126 pKa = 9.97 LRR128 pKa = 11.84 YY129 pKa = 9.29 KK130 pKa = 9.28 KK131 pKa = 8.89 TLEE134 pKa = 3.86 SLQKK138 pKa = 10.33 FRR140 pKa = 11.84 SDD142 pKa = 5.42 DD143 pKa = 3.75 LDD145 pKa = 4.03 GSCGVWICGPPRR157 pKa = 11.84 IGKK160 pKa = 9.76 DD161 pKa = 3.2 YY162 pKa = 11.1 AVRR165 pKa = 11.84 KK166 pKa = 9.24 IGNVYY171 pKa = 10.38 CKK173 pKa = 10.45 ALNKK177 pKa = 8.98 WWDD180 pKa = 3.78 GYY182 pKa = 11.03 LGEE185 pKa = 4.7 PNVLISDD192 pKa = 4.08 VEE194 pKa = 3.97 PDD196 pKa = 3.05 HH197 pKa = 6.64 FKK199 pKa = 10.46 WIGYY203 pKa = 7.28 FLKK206 pKa = 9.94 IWSDD210 pKa = 3.86 RR211 pKa = 11.84 YY212 pKa = 10.36 PFIAEE217 pKa = 3.91 IKK219 pKa = 8.93 GSSMKK224 pKa = 9.89 IRR226 pKa = 11.84 PEE228 pKa = 4.19 KK229 pKa = 10.72 IFVTSNFRR237 pKa = 11.84 LSDD240 pKa = 3.7 CCNGEE245 pKa = 3.82 ILGALQSRR253 pKa = 11.84 FTIYY257 pKa = 11.43 DD258 pKa = 3.1 MFDD261 pKa = 2.86 NVVRR265 pKa = 11.84 KK266 pKa = 9.8 RR267 pKa = 11.84 PVVAASTCVFDD278 pKa = 5.9 LLVEE282 pKa = 4.46 NGDD285 pKa = 3.46 ACLEE289 pKa = 4.23 KK290 pKa = 10.98 EE291 pKa = 4.4 NVPPAVQEE299 pKa = 4.26 AVATTSKK306 pKa = 10.54 QPTRR310 pKa = 11.84 AEE312 pKa = 4.05 EE313 pKa = 4.26 EE314 pKa = 4.15 EE315 pKa = 4.27 LQDD318 pKa = 3.67 FQVVKK323 pKa = 10.66 KK324 pKa = 10.18 KK325 pKa = 9.94 PRR327 pKa = 11.84 KK328 pKa = 9.45 AKK330 pKa = 10.56 SSS332 pKa = 3.41
Molecular weight: 37.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.48
IPC2_protein 7.819
IPC_protein 7.614
Toseland 7.146
ProMoST 8.214
Dawson 8.361
Bjellqvist 8.77
Wikipedia 8.273
Rodwell 8.404
Grimsley 7.117
Solomon 8.434
Lehninger 8.448
Nozaki 9.048
DTASelect 8.448
Thurlkill 8.536
EMBOSS 8.58
Sillero 8.873
Patrickios 4.406
IPC_peptide 8.434
IPC2_peptide 7.673
IPC2.peptide.svr19 7.853
Protein with the highest isoelectric point:
>tr|K0A2Q4|K0A2Q4_9VIRU Replication-associated protein OS=Dragonfly circularisvirus OX=1234872 PE=4 SV=1
MM1 pKa = 6.75 VKK3 pKa = 9.74 SWGRR7 pKa = 11.84 CSRR10 pKa = 11.84 VLQSTTCLIMLSEE23 pKa = 4.39 SVLLLLRR30 pKa = 11.84 LLVCLICLLKK40 pKa = 10.08 MVMRR44 pKa = 11.84 AWRR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 MYY51 pKa = 10.46 RR52 pKa = 11.84 LRR54 pKa = 11.84 YY55 pKa = 8.25 RR56 pKa = 11.84 KK57 pKa = 9.26 RR58 pKa = 11.84 WQQRR62 pKa = 11.84 RR63 pKa = 11.84 NSRR66 pKa = 11.84 LGRR69 pKa = 11.84 RR70 pKa = 11.84 KK71 pKa = 9.8 RR72 pKa = 11.84 SYY74 pKa = 9.71 KK75 pKa = 8.67 TFKK78 pKa = 10.24 SSKK81 pKa = 10.16 RR82 pKa = 11.84 NLAKK86 pKa = 10.63 LNQVKK91 pKa = 7.88 VTVRR95 pKa = 11.84 DD96 pKa = 3.41 LDD98 pKa = 3.47 YY99 pKa = 11.05 HH100 pKa = 6.8 IIKK103 pKa = 10.13 IPYY106 pKa = 8.05 TGGDD110 pKa = 3.31 PRR112 pKa = 11.84 QIGSEE117 pKa = 3.86 NAEE120 pKa = 4.07 MFKK123 pKa = 10.5 TDD125 pKa = 3.6 VPVHH129 pKa = 5.42 ALKK132 pKa = 10.38 MKK134 pKa = 10.78 LFTLNLQRR142 pKa = 11.84 YY143 pKa = 8.0 KK144 pKa = 10.21 WIKK147 pKa = 9.08 FNYY150 pKa = 8.06 FSFYY154 pKa = 9.21 ITADD158 pKa = 3.88 CISYY162 pKa = 10.41 DD163 pKa = 2.99 IYY165 pKa = 10.63 PYY167 pKa = 10.7 KK168 pKa = 10.55 DD169 pKa = 3.56 EE170 pKa = 4.03 QSKK173 pKa = 9.18 TSGLLSPGINEE184 pKa = 4.41 FFKK187 pKa = 11.0 HH188 pKa = 6.01 LPFYY192 pKa = 9.98 LQWDD196 pKa = 4.02 LDD198 pKa = 3.92 VTFDD202 pKa = 4.38 NRR204 pKa = 11.84 DD205 pKa = 3.49 LTEE208 pKa = 3.79 VNYY211 pKa = 10.58 PNDD214 pKa = 3.12 VHH216 pKa = 6.77 AKK218 pKa = 9.78 KK219 pKa = 10.46 IFIGSRR225 pKa = 11.84 KK226 pKa = 8.4 PAVFKK231 pKa = 10.64 YY232 pKa = 9.51 VIPQHH237 pKa = 5.27 LRR239 pKa = 11.84 KK240 pKa = 9.68 YY241 pKa = 9.62 LPCGVVKK248 pKa = 10.72 SQVTTKK254 pKa = 10.43 PLPLYY259 pKa = 10.05 LQEE262 pKa = 5.11 LFKK265 pKa = 11.04 SDD267 pKa = 3.49 NFRR270 pKa = 11.84 VPSVFYY276 pKa = 11.06 GGVTNLMRR284 pKa = 11.84 ALDD287 pKa = 4.02 LFYY290 pKa = 11.04 NKK292 pKa = 9.59 PSEE295 pKa = 4.23 GRR297 pKa = 11.84 DD298 pKa = 3.32 VPLRR302 pKa = 11.84 FVLTVKK308 pKa = 10.51 CYY310 pKa = 11.36 ANVTFKK316 pKa = 11.33 GIDD319 pKa = 3.4 LVV321 pKa = 3.86
Molecular weight: 38.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.249
IPC2_protein 9.575
IPC_protein 9.706
Toseland 10.16
ProMoST 9.853
Dawson 10.365
Bjellqvist 10.043
Wikipedia 10.54
Rodwell 10.774
Grimsley 10.438
Solomon 10.379
Lehninger 10.35
Nozaki 10.175
DTASelect 10.028
Thurlkill 10.204
EMBOSS 10.555
Sillero 10.277
Patrickios 10.335
IPC_peptide 10.379
IPC2_peptide 8.858
IPC2.peptide.svr19 8.525
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
653
321
332
326.5
37.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.441 ± 1.245
3.063 ± 0.671
5.666 ± 0.518
4.288 ± 1.366
5.36 ± 0.188
5.36 ± 0.996
1.378 ± 0.136
4.9 ± 0.173
9.035 ± 0.001
9.648 ± 1.902
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.685 ± 0.614
3.828 ± 0.405
5.207 ± 0.406
3.063 ± 0.277
7.198 ± 1.16
7.044 ± 0.619
5.207 ± 0.169
7.351 ± 0.57
1.685 ± 0.097
4.594 ± 1.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here