Chaetoceros protobacilladnavirus 4
Average proteome isoelectric point is 7.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E3WH29|E3WH29_9VIRU Putative replication-associated protein OS=Chaetoceros protobacilladnavirus 4 OX=2170143 PE=4 SV=1
MM1 pKa = 7.61 HH2 pKa = 7.31 FRR4 pKa = 11.84 NMSNNSFDD12 pKa = 3.76 IQEE15 pKa = 5.05 LICDD19 pKa = 4.48 DD20 pKa = 4.47 ASIDD24 pKa = 3.76 CSISINTTPSVDD36 pKa = 3.97 FLEE39 pKa = 4.87 TEE41 pKa = 4.31 LKK43 pKa = 10.78 EE44 pKa = 4.22 PTNNTLISRR53 pKa = 11.84 AILTYY58 pKa = 9.92 FPPTSDD64 pKa = 3.07 AKK66 pKa = 10.21 YY67 pKa = 10.46 LEE69 pKa = 4.5 PEE71 pKa = 4.08 FMLGQDD77 pKa = 4.03 IRR79 pKa = 11.84 RR80 pKa = 11.84 LSNWWSQYY88 pKa = 7.88 EE89 pKa = 4.03 KK90 pKa = 10.44 CPEE93 pKa = 3.95 SGAVHH98 pKa = 5.45 IHH100 pKa = 6.76 AYY102 pKa = 10.68 AEE104 pKa = 4.76 FKK106 pKa = 10.86 RR107 pKa = 11.84 EE108 pKa = 3.69 LRR110 pKa = 11.84 PRR112 pKa = 11.84 FNLLDD117 pKa = 3.64 EE118 pKa = 5.66 LIRR121 pKa = 11.84 SHH123 pKa = 6.73 VDD125 pKa = 2.6 CWNCRR130 pKa = 11.84 TARR133 pKa = 11.84 KK134 pKa = 9.69 SSEE137 pKa = 4.02 SQRR140 pKa = 11.84 QGAINYY146 pKa = 5.81 VHH148 pKa = 7.06 KK149 pKa = 10.95 FEE151 pKa = 4.29 TRR153 pKa = 11.84 LDD155 pKa = 3.63 GFQPFTWPKK164 pKa = 9.94 NEE166 pKa = 4.25 SNAKK170 pKa = 9.6 FDD172 pKa = 3.92 PSFVRR177 pKa = 11.84 TNSKK181 pKa = 8.91 QNKK184 pKa = 8.58 RR185 pKa = 11.84 KK186 pKa = 10.12 ARR188 pKa = 11.84 DD189 pKa = 3.44 DD190 pKa = 4.49 AIEE193 pKa = 3.9 EE194 pKa = 3.69 QRR196 pKa = 11.84 QYY198 pKa = 11.01 IEE200 pKa = 4.12 SKK202 pKa = 9.72 PRR204 pKa = 11.84 IWTWDD209 pKa = 3.36 QILHH213 pKa = 5.84 EE214 pKa = 4.65 NEE216 pKa = 3.78 ASKK219 pKa = 11.12 KK220 pKa = 10.7 LLVTCSWGKK229 pKa = 9.75 KK230 pKa = 6.24 YY231 pKa = 10.62 HH232 pKa = 6.38 EE233 pKa = 4.89 GRR235 pKa = 11.84 HH236 pKa = 5.65 AEE238 pKa = 4.14 DD239 pKa = 3.29 KK240 pKa = 11.0 RR241 pKa = 11.84 RR242 pKa = 11.84 DD243 pKa = 3.52 VTDD246 pKa = 3.59 VIIYY250 pKa = 9.63 YY251 pKa = 10.43 GAGGTGKK258 pKa = 7.43 TTLCMEE264 pKa = 4.41 YY265 pKa = 10.24 NQTSSLEE272 pKa = 4.11 PRR274 pKa = 11.84 VEE276 pKa = 3.56 RR277 pKa = 11.84 YY278 pKa = 8.52 YY279 pKa = 10.96 RR280 pKa = 11.84 RR281 pKa = 11.84 NPDD284 pKa = 4.5 DD285 pKa = 4.03 GNFWGGGRR293 pKa = 11.84 TAYY296 pKa = 9.76 KK297 pKa = 8.82 GQRR300 pKa = 11.84 VIHH303 pKa = 6.06 FEE305 pKa = 4.02 EE306 pKa = 5.18 FSGQEE311 pKa = 3.48 PFSRR315 pKa = 11.84 LKK317 pKa = 10.11 EE318 pKa = 3.9 VCDD321 pKa = 3.38 IGKK324 pKa = 9.09 PGPSVNIKK332 pKa = 10.38 GAGVDD337 pKa = 4.25 LNHH340 pKa = 6.34 HH341 pKa = 5.45 TVMFSSNVHH350 pKa = 5.23 PAGWYY355 pKa = 8.66 RR356 pKa = 11.84 NLWRR360 pKa = 11.84 DD361 pKa = 3.71 DD362 pKa = 3.67 PKK364 pKa = 10.89 QFHH367 pKa = 6.28 PFWRR371 pKa = 11.84 RR372 pKa = 11.84 VTKK375 pKa = 10.38 VVFFPSHH382 pKa = 6.56 RR383 pKa = 11.84 PDD385 pKa = 3.01 GSMNIPDD392 pKa = 4.62 EE393 pKa = 5.01 DD394 pKa = 4.42 NPPHH398 pKa = 6.78 MIDD401 pKa = 4.98 QTDD404 pKa = 2.86 EE405 pKa = 3.8 WKK407 pKa = 10.81 ALNGSYY413 pKa = 8.81 EE414 pKa = 3.97 ACMEE418 pKa = 4.51 HH419 pKa = 8.19 ADD421 pKa = 3.62 EE422 pKa = 4.59 HH423 pKa = 6.04 WPVRR427 pKa = 11.84 EE428 pKa = 3.8 VSVDD432 pKa = 3.48 GFVKK436 pKa = 10.54 SFNHH440 pKa = 5.54 SHH442 pKa = 4.64 QTSAA446 pKa = 4.16
Molecular weight: 52.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.128
IPC2_protein 6.046
IPC_protein 6.135
Toseland 6.517
ProMoST 6.547
Dawson 6.465
Bjellqvist 6.453
Wikipedia 6.491
Rodwell 6.465
Grimsley 6.664
Solomon 6.465
Lehninger 6.465
Nozaki 6.751
DTASelect 6.927
Thurlkill 6.956
EMBOSS 6.927
Sillero 6.854
Patrickios 4.291
IPC_peptide 6.478
IPC2_peptide 6.781
IPC2.peptide.svr19 6.745
Protein with the highest isoelectric point:
>tr|E3WH28|E3WH28_9VIRU Uncharacterized protein OS=Chaetoceros protobacilladnavirus 4 OX=2170143 PE=4 SV=1
MM1 pKa = 6.8 VQHH4 pKa = 7.2 DD5 pKa = 3.98 VQNIGRR11 pKa = 11.84 KK12 pKa = 8.72 RR13 pKa = 11.84 DD14 pKa = 3.59 FRR16 pKa = 11.84 TVTPEE21 pKa = 3.93 APLEE25 pKa = 4.21 MPSGQRR31 pKa = 11.84 LHH33 pKa = 5.5 YY34 pKa = 9.69 TEE36 pKa = 5.28 DD37 pKa = 3.59 LAVIGPEE44 pKa = 4.16 PEE46 pKa = 3.77 RR47 pKa = 11.84 APYY50 pKa = 10.27 DD51 pKa = 3.46 IVPFAGDD58 pKa = 3.41 EE59 pKa = 4.29 LKK61 pKa = 11.03 EE62 pKa = 3.92 QMTLIRR68 pKa = 11.84 NNGMTEE74 pKa = 3.86 QQAISYY80 pKa = 7.28 FTDD83 pKa = 3.62 YY84 pKa = 11.44 AEE86 pKa = 4.63 KK87 pKa = 10.33 SHH89 pKa = 7.82 LGEE92 pKa = 4.66 FSAAAYY98 pKa = 7.6 SAPSGVAIRR107 pKa = 11.84 VNPHH111 pKa = 5.88 TGLKK115 pKa = 9.46 EE116 pKa = 3.69 AFIAGTRR123 pKa = 11.84 GPLDD127 pKa = 3.04 WGRR130 pKa = 11.84 NIIEE134 pKa = 4.41 GADD137 pKa = 3.85 SIVSAGLHH145 pKa = 5.26 KK146 pKa = 10.04 VTNIMPKK153 pKa = 10.48 GEE155 pKa = 4.3 LRR157 pKa = 11.84 NKK159 pKa = 10.4 AEE161 pKa = 3.75 AAANVLEE168 pKa = 4.33 NVSVPGRR175 pKa = 11.84 ISKK178 pKa = 10.19 ILTRR182 pKa = 11.84 SEE184 pKa = 4.07 GEE186 pKa = 3.87 EE187 pKa = 3.58 LAEE190 pKa = 4.16 TFRR193 pKa = 11.84 EE194 pKa = 4.02 EE195 pKa = 4.38 GVQVIYY201 pKa = 10.33 GHH203 pKa = 6.03 SRR205 pKa = 11.84 AGAILQYY212 pKa = 10.46 FPEE215 pKa = 4.49 EE216 pKa = 4.19 EE217 pKa = 4.22 FTTIALDD224 pKa = 3.51 GANIVGHH231 pKa = 7.1 DD232 pKa = 4.29 PMSAPNFSSDD242 pKa = 4.55 DD243 pKa = 3.34 IFSRR247 pKa = 11.84 TIGIGSRR254 pKa = 11.84 KK255 pKa = 9.31 RR256 pKa = 11.84 VEE258 pKa = 3.73 LPGRR262 pKa = 11.84 SFHH265 pKa = 7.05 KK266 pKa = 10.49 AWEE269 pKa = 4.41 TKK271 pKa = 9.87 AKK273 pKa = 9.72 KK274 pKa = 10.38 AKK276 pKa = 9.83 ADD278 pKa = 3.47 KK279 pKa = 10.48 KK280 pKa = 11.14 AEE282 pKa = 4.0 DD283 pKa = 3.61 RR284 pKa = 11.84 KK285 pKa = 10.37 KK286 pKa = 11.09 SKK288 pKa = 10.79 LGVPLAEE295 pKa = 4.46 LKK297 pKa = 10.88 KK298 pKa = 10.55 EE299 pKa = 4.26 FYY301 pKa = 10.2 EE302 pKa = 4.3 QADD305 pKa = 3.58 KK306 pKa = 10.42 ATRR309 pKa = 11.84 RR310 pKa = 11.84 QHH312 pKa = 4.87 MKK314 pKa = 9.94 LVEE317 pKa = 3.94 RR318 pKa = 11.84 AKK320 pKa = 10.27 TKK322 pKa = 10.03 IGKK325 pKa = 9.31 KK326 pKa = 9.55 FAANLAKK333 pKa = 10.72 YY334 pKa = 9.22 IAKK337 pKa = 10.12 QSAEE341 pKa = 4.22 TAIKK345 pKa = 10.03 ALPGGSYY352 pKa = 10.7 LPIGQASAPVAAYY365 pKa = 8.32 PPLPRR370 pKa = 11.84 LVEE373 pKa = 4.27 GDD375 pKa = 3.74 LRR377 pKa = 11.84 SGTVVPYY384 pKa = 9.19 TKK386 pKa = 10.32 KK387 pKa = 10.17 RR388 pKa = 11.84 NKK390 pKa = 10.46 KK391 pKa = 10.09 KK392 pKa = 8.14 MTSARR397 pKa = 11.84 KK398 pKa = 9.35 SKK400 pKa = 9.84 YY401 pKa = 10.17 ARR403 pKa = 11.84 LNDD406 pKa = 4.07 EE407 pKa = 4.88 LPHH410 pKa = 6.39 MGDD413 pKa = 2.92 IDD415 pKa = 3.6
Molecular weight: 45.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.824
IPC2_protein 8.756
IPC_protein 8.653
Toseland 9.545
ProMoST 9.209
Dawson 9.765
Bjellqvist 9.399
Wikipedia 9.911
Rodwell 10.16
Grimsley 9.838
Solomon 9.794
Lehninger 9.75
Nozaki 9.502
DTASelect 9.399
Thurlkill 9.604
EMBOSS 9.955
Sillero 9.663
Patrickios 5.614
IPC_peptide 9.794
IPC2_peptide 7.761
IPC2.peptide.svr19 7.888
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1233
372
446
411.0
46.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.948 ± 1.494
1.217 ± 0.451
5.758 ± 0.477
6.569 ± 1.009
4.055 ± 0.48
6.407 ± 0.517
3.244 ± 0.441
5.353 ± 0.246
7.218 ± 0.685
6.002 ± 0.456
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.109 ± 0.143
5.921 ± 1.045
5.272 ± 0.408
3.731 ± 0.376
6.569 ± 0.393
7.218 ± 0.424
5.353 ± 0.184
5.434 ± 0.275
1.541 ± 0.592
3.082 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here