Pseudomonas phage phi1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U4JX40|A0A0U4JX40_9CAUD ATP-dependent Clp protease ATP-binding subunit OS=Pseudomonas phage phi1 OX=1754216 PE=4 SV=1
MM1 pKa = 8.79SMIPLDD7 pKa = 3.78TAKK10 pKa = 10.86SFLDD14 pKa = 5.15VIHH17 pKa = 7.24DD18 pKa = 3.59WDD20 pKa = 4.08DD21 pKa = 3.7AKK23 pKa = 11.25LQLLLDD29 pKa = 4.12GAEE32 pKa = 4.76DD33 pKa = 3.88EE34 pKa = 5.24ACQFMWRR41 pKa = 11.84QSLDD45 pKa = 3.35GLCNCEE51 pKa = 3.91EE52 pKa = 4.14SSEE55 pKa = 4.49VVSSEE60 pKa = 3.65PGIPPSVVIGVLLLLQASYY79 pKa = 10.56QAAPEE84 pKa = 4.39EE85 pKa = 4.28IATLRR90 pKa = 11.84KK91 pKa = 9.12AAEE94 pKa = 4.35VKK96 pKa = 10.42LMPYY100 pKa = 10.05RR101 pKa = 11.84CGLGVV106 pKa = 3.15

Molecular weight: 11.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U4B0K0|A0A0U4B0K0_9CAUD Uncharacterized protein OS=Pseudomonas phage phi1 OX=1754216 PE=4 SV=1
MM1 pKa = 7.18NRR3 pKa = 11.84HH4 pKa = 6.43FKK6 pKa = 10.6RR7 pKa = 11.84IMLYY11 pKa = 8.59TKK13 pKa = 9.68RR14 pKa = 11.84TLLGAMVAILIVFKK28 pKa = 10.99AIDD31 pKa = 3.92LGGAITGEE39 pKa = 4.07ATAEE43 pKa = 3.81QPITHH48 pKa = 7.21LSAAGRR54 pKa = 3.72

Molecular weight: 5.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

13365

19

909

193.7

21.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.857 ± 0.623

1.302 ± 0.161

6.098 ± 0.252

7.063 ± 0.425

3.479 ± 0.179

7.594 ± 0.366

1.826 ± 0.189

5.657 ± 0.312

4.886 ± 0.302

8.23 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.776 ± 0.187

3.277 ± 0.196

4.212 ± 0.26

3.981 ± 0.248

6.599 ± 0.288

6.397 ± 0.387

5.208 ± 0.225

5.918 ± 0.201

1.713 ± 0.141

2.926 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski