bacterium HR32

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 7.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2139 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5ZYX6|A0A2H5ZYX6_9BACT Endolytic murein transglycosylase OS=bacterium HR32 OX=2035427 GN=mltG PE=3 SV=1
MM1 pKa = 7.14GTGFDD6 pKa = 3.38GCGRR10 pKa = 11.84CVEE13 pKa = 4.5GYY15 pKa = 10.25VGEE18 pKa = 4.74FGQQGGIEE26 pKa = 4.37GEE28 pKa = 4.53FGQSGCSEE36 pKa = 4.44DD37 pKa = 3.83GAVGRR42 pKa = 11.84GEE44 pKa = 4.65SPDD47 pKa = 3.37GVQNLGGVVDD57 pKa = 4.4GEE59 pKa = 4.5RR60 pKa = 11.84GGHH63 pKa = 6.45LGFVPDD69 pKa = 4.47EE70 pKa = 4.53APAAADD76 pKa = 3.63DD77 pKa = 4.49SEE79 pKa = 5.33GSGALQVAHH88 pKa = 7.06AGDD91 pKa = 4.39DD92 pKa = 3.76VSGEE96 pKa = 4.04AVGVGVGDD104 pKa = 4.01GGG106 pKa = 3.81

Molecular weight: 10.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5ZX65|A0A2H5ZX65_9BACT Oligopeptide-binding protein AppA OS=bacterium HR32 OX=2035427 GN=appA_1 PE=4 SV=1
MM1 pKa = 7.52RR2 pKa = 11.84RR3 pKa = 11.84SSSSTRR9 pKa = 11.84SRR11 pKa = 11.84HH12 pKa = 5.19SRR14 pKa = 11.84SLPSAVRR21 pKa = 11.84RR22 pKa = 11.84SRR24 pKa = 11.84PHH26 pKa = 6.78LAQKK30 pKa = 9.68GWLMGLMNPRR40 pKa = 11.84VPAAPRR46 pKa = 11.84IRR48 pKa = 11.84YY49 pKa = 8.51VRR51 pKa = 11.84AGSSPEE57 pKa = 3.54RR58 pKa = 11.84SRR60 pKa = 11.84GSRR63 pKa = 11.84GPKK66 pKa = 9.22ASSSRR71 pKa = 11.84RR72 pKa = 11.84TVSSVRR78 pKa = 11.84TTLPSAKK85 pKa = 9.97SATSPKK91 pKa = 10.48GIISMNRR98 pKa = 11.84TSHH101 pKa = 4.69GRR103 pKa = 11.84SRR105 pKa = 11.84VSAASGTTASSLNPRR120 pKa = 11.84TTTVLSLTGCRR131 pKa = 11.84PASSAARR138 pKa = 11.84IPSRR142 pKa = 11.84TSCSRR147 pKa = 11.84PPRR150 pKa = 11.84VMRR153 pKa = 11.84RR154 pKa = 11.84NCSGSRR160 pKa = 11.84VSRR163 pKa = 11.84LTLTRR168 pKa = 11.84RR169 pKa = 11.84SPASASGRR177 pKa = 11.84ARR179 pKa = 11.84SASSTPFVVSATSSMPGMAAMPRR202 pKa = 11.84TSSTSPGRR210 pKa = 11.84TVGSPPVSRR219 pKa = 11.84TFWTPSSAAMRR230 pKa = 11.84VSRR233 pKa = 11.84RR234 pKa = 11.84ISWSCSSSAAGRR246 pKa = 11.84KK247 pKa = 9.08RR248 pKa = 11.84MPSAGMQQ255 pKa = 3.11

Molecular weight: 27.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2139

0

2139

598707

29

1107

279.9

30.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.784 ± 0.075

0.894 ± 0.018

4.468 ± 0.036

6.486 ± 0.059

3.078 ± 0.036

9.071 ± 0.058

2.127 ± 0.037

2.41 ± 0.04

1.468 ± 0.028

11.422 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.597 ± 0.021

1.494 ± 0.027

6.205 ± 0.05

3.157 ± 0.038

10.198 ± 0.061

4.174 ± 0.046

4.419 ± 0.036

10.677 ± 0.059

1.628 ± 0.029

2.242 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski