Temnothorax curvispinosus
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 21925 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6J1Q4E3|A0A6J1Q4E3_9HYME Sodium channel protein OS=Temnothorax curvispinosus OX=300111 GN=LOC112457128 PE=3 SV=1
MM1 pKa = 7.1 LTKK4 pKa = 10.62 DD5 pKa = 3.69 SNNEE9 pKa = 3.82 EE10 pKa = 4.28 KK11 pKa = 10.12 TVDD14 pKa = 4.16 ALNTCYY20 pKa = 10.69 DD21 pKa = 3.91 KK22 pKa = 11.42 EE23 pKa = 4.68 SVMLTNGCNNEE34 pKa = 4.05 EE35 pKa = 4.23 TTIDD39 pKa = 4.52 ALNTCYY45 pKa = 10.78 GINTKK50 pKa = 10.55 SYY52 pKa = 10.09 IDD54 pKa = 4.0 KK55 pKa = 10.94 NNEE58 pKa = 3.78 NNSEE62 pKa = 4.22 SVMLTKK68 pKa = 10.65 DD69 pKa = 3.49 SNNEE73 pKa = 3.53 EE74 pKa = 4.21 TTIDD78 pKa = 4.27 ALNTCYY84 pKa = 10.7 DD85 pKa = 3.91 KK86 pKa = 11.42 EE87 pKa = 4.64 SVMLTNDD94 pKa = 3.42 SNNEE98 pKa = 3.77 EE99 pKa = 4.28 TTDD102 pKa = 4.15 DD103 pKa = 4.49 VLNTCYY109 pKa = 10.5 GYY111 pKa = 10.54 DD112 pKa = 3.31 ISTLDD117 pKa = 3.62 VIFMDD122 pKa = 5.11 KK123 pKa = 10.41 YY124 pKa = 10.18 RR125 pKa = 11.84 KK126 pKa = 7.92 TMNIMNKK133 pKa = 10.07 DD134 pKa = 3.19 CDD136 pKa = 3.68 IEE138 pKa = 4.46 GKK140 pKa = 10.17 SDD142 pKa = 3.79 ATDD145 pKa = 3.2 TCNTVEE151 pKa = 4.44 NDD153 pKa = 4.17 DD154 pKa = 4.6 IAGKK158 pKa = 10.03 DD159 pKa = 3.2
Molecular weight: 17.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.846
IPC_protein 3.846
Toseland 3.617
ProMoST 3.961
Dawson 3.834
Bjellqvist 4.012
Wikipedia 3.757
Rodwell 3.668
Grimsley 3.528
Solomon 3.821
Lehninger 3.783
Nozaki 3.948
DTASelect 4.177
Thurlkill 3.681
EMBOSS 3.77
Sillero 3.961
Patrickios 1.888
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.875
Protein with the highest isoelectric point:
>tr|A0A6J1PSB2|A0A6J1PSB2_9HYME carbonic anhydrase 9-like OS=Temnothorax curvispinosus OX=300111 GN=LOC112454783 PE=3 SV=1
MM1 pKa = 7.47 SPLNRR6 pKa = 11.84 GPLMGPRR13 pKa = 11.84 LPPAGARR20 pKa = 11.84 QLPPPRR26 pKa = 11.84 FVGRR30 pKa = 11.84 ITPHH34 pKa = 5.68 GMPPRR39 pKa = 11.84 MPPLPPLNPMFGPRR53 pKa = 11.84 QRR55 pKa = 11.84 LPLPPRR61 pKa = 11.84 PAGGVSRR68 pKa = 11.84 PNGMLPRR75 pKa = 11.84 FPAGPRR81 pKa = 11.84 SRR83 pKa = 11.84 GMAPIVPSMGPRR95 pKa = 11.84 GSGPPRR101 pKa = 11.84 GPPMRR106 pKa = 11.84 PWPRR110 pKa = 11.84 RR111 pKa = 11.84 MPLPPPPLPLQMVPPMRR128 pKa = 11.84 PRR130 pKa = 11.84 YY131 pKa = 9.28 NAGNGNAKK139 pKa = 10.24 AKK141 pKa = 10.23 TMGNAKK147 pKa = 9.58 KK148 pKa = 10.83 VKK150 pKa = 10.43 VEE152 pKa = 3.87
Molecular weight: 16.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.464
IPC2_protein 11.125
IPC_protein 12.603
Toseland 12.764
ProMoST 13.247
Dawson 12.764
Bjellqvist 12.749
Wikipedia 13.232
Rodwell 12.369
Grimsley 12.793
Solomon 13.261
Lehninger 13.159
Nozaki 12.764
DTASelect 12.749
Thurlkill 12.764
EMBOSS 13.261
Sillero 12.764
Patrickios 12.091
IPC_peptide 13.261
IPC2_peptide 12.252
IPC2.peptide.svr19 9.105
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15254
6671
21925
14401447
34
16248
656.9
73.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.451 ± 0.015
1.865 ± 0.018
5.614 ± 0.014
6.907 ± 0.028
3.359 ± 0.013
5.431 ± 0.019
2.516 ± 0.008
5.545 ± 0.013
6.262 ± 0.027
8.949 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.252 ± 0.008
4.884 ± 0.014
5.154 ± 0.021
4.43 ± 0.022
5.893 ± 0.016
8.225 ± 0.022
6.134 ± 0.013
6.066 ± 0.013
1.088 ± 0.005
2.974 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here