Amino acid dipepetide frequency for Lactococcus virus Bibb29

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.037AlaAla: 1.037 ± 0.357
0.115AlaCys: 0.115 ± 0.125
2.419AlaAsp: 2.419 ± 0.554
4.493AlaGlu: 4.493 ± 0.769
3.111AlaPhe: 3.111 ± 0.777
4.493AlaGly: 4.493 ± 0.985
0.922AlaHis: 0.922 ± 0.284
4.493AlaIle: 4.493 ± 1.323
5.876AlaLys: 5.876 ± 0.907
6.106AlaLeu: 6.106 ± 0.896
2.65AlaMet: 2.65 ± 0.745
4.954AlaAsn: 4.954 ± 0.753
0.806AlaPro: 0.806 ± 0.341
2.419AlaGln: 2.419 ± 0.543
2.535AlaArg: 2.535 ± 0.57
3.456AlaSer: 3.456 ± 0.866
3.341AlaThr: 3.341 ± 0.799
4.954AlaVal: 4.954 ± 1.134
1.728AlaTrp: 1.728 ± 0.537
2.074AlaTyr: 2.074 ± 0.464
0.0AlaXaa: 0.0 ± 0.0
Cys
0.23CysAla: 0.23 ± 0.146
0.0CysCys: 0.0 ± 0.0
0.23CysAsp: 0.23 ± 0.191
0.23CysGlu: 0.23 ± 0.186
0.115CysPhe: 0.115 ± 0.137
0.806CysGly: 0.806 ± 0.344
0.23CysHis: 0.23 ± 0.18
0.691CysIle: 0.691 ± 0.321
0.806CysLys: 0.806 ± 0.366
0.23CysLeu: 0.23 ± 0.17
0.0CysMet: 0.0 ± 0.0
0.346CysAsn: 0.346 ± 0.2
0.461CysPro: 0.461 ± 0.211
0.461CysGln: 0.461 ± 0.217
0.461CysArg: 0.461 ± 0.246
0.23CysSer: 0.23 ± 0.159
0.461CysThr: 0.461 ± 0.235
0.346CysVal: 0.346 ± 0.195
0.115CysTrp: 0.115 ± 0.137
0.461CysTyr: 0.461 ± 0.24
0.0CysXaa: 0.0 ± 0.0
Asp
1.959AspAla: 1.959 ± 0.547
0.23AspCys: 0.23 ± 0.21
2.88AspAsp: 2.88 ± 0.721
4.147AspGlu: 4.147 ± 0.747
3.456AspPhe: 3.456 ± 0.502
2.765AspGly: 2.765 ± 0.451
0.346AspHis: 0.346 ± 0.207
4.839AspIle: 4.839 ± 0.771
5.53AspLys: 5.53 ± 0.683
5.069AspLeu: 5.069 ± 0.837
1.037AspMet: 1.037 ± 0.288
3.917AspAsn: 3.917 ± 0.72
1.613AspPro: 1.613 ± 0.506
0.576AspGln: 0.576 ± 0.368
2.189AspArg: 2.189 ± 0.479
2.995AspSer: 2.995 ± 0.516
5.415AspThr: 5.415 ± 0.765
3.802AspVal: 3.802 ± 0.787
0.691AspTrp: 0.691 ± 0.245
2.765AspTyr: 2.765 ± 0.505
0.0AspXaa: 0.0 ± 0.0
Glu
3.571GluAla: 3.571 ± 0.72
0.23GluCys: 0.23 ± 0.18
3.456GluAsp: 3.456 ± 0.602
5.645GluGlu: 5.645 ± 1.221
3.571GluPhe: 3.571 ± 0.592
1.959GluGly: 1.959 ± 0.403
1.382GluHis: 1.382 ± 0.473
6.336GluIle: 6.336 ± 1.042
7.143GluLys: 7.143 ± 1.535
10.369GluLeu: 10.369 ± 1.686
2.765GluMet: 2.765 ± 0.656
4.954GluAsn: 4.954 ± 0.746
1.613GluPro: 1.613 ± 0.427
4.263GluGln: 4.263 ± 0.931
2.88GluArg: 2.88 ± 0.654
4.378GluSer: 4.378 ± 0.678
4.378GluThr: 4.378 ± 0.641
4.378GluVal: 4.378 ± 0.656
1.037GluTrp: 1.037 ± 0.349
3.111GluTyr: 3.111 ± 0.852
0.0GluXaa: 0.0 ± 0.0
Phe
3.687PheAla: 3.687 ± 0.888
0.23PheCys: 0.23 ± 0.176
3.456PheAsp: 3.456 ± 0.666
2.535PheGlu: 2.535 ± 0.594
2.304PhePhe: 2.304 ± 0.877
2.419PheGly: 2.419 ± 0.464
0.346PheHis: 0.346 ± 0.199
3.341PheIle: 3.341 ± 0.687
4.493PheLys: 4.493 ± 0.708
2.304PheLeu: 2.304 ± 0.464
0.922PheMet: 0.922 ± 0.323
2.765PheAsn: 2.765 ± 0.782
0.691PhePro: 0.691 ± 0.279
1.037PheGln: 1.037 ± 0.379
1.152PheArg: 1.152 ± 0.351
4.378PheSer: 4.378 ± 1.111
2.765PheThr: 2.765 ± 0.505
2.765PheVal: 2.765 ± 0.447
0.461PheTrp: 0.461 ± 0.231
1.267PheTyr: 1.267 ± 0.422
0.0PheXaa: 0.0 ± 0.0
Gly
3.802GlyAla: 3.802 ± 1.209
0.346GlyCys: 0.346 ± 0.216
3.111GlyAsp: 3.111 ± 0.673
4.608GlyGlu: 4.608 ± 0.799
2.765GlyPhe: 2.765 ± 0.854
4.493GlyGly: 4.493 ± 1.157
0.576GlyHis: 0.576 ± 0.272
4.032GlyIle: 4.032 ± 1.339
6.106GlyLys: 6.106 ± 0.592
5.76GlyLeu: 5.76 ± 0.867
0.922GlyMet: 0.922 ± 0.357
3.571GlyAsn: 3.571 ± 0.587
0.23GlyPro: 0.23 ± 0.175
2.304GlyGln: 2.304 ± 0.501
1.613GlyArg: 1.613 ± 0.411
4.608GlySer: 4.608 ± 0.872
2.65GlyThr: 2.65 ± 0.624
5.53GlyVal: 5.53 ± 1.302
1.037GlyTrp: 1.037 ± 0.321
3.111GlyTyr: 3.111 ± 0.571
0.0GlyXaa: 0.0 ± 0.0
His
0.576HisAla: 0.576 ± 0.231
0.461HisCys: 0.461 ± 0.338
0.806HisAsp: 0.806 ± 0.244
0.576HisGlu: 0.576 ± 0.233
0.691HisPhe: 0.691 ± 0.391
1.382HisGly: 1.382 ± 0.34
0.0HisHis: 0.0 ± 0.0
0.576HisIle: 0.576 ± 0.295
1.037HisLys: 1.037 ± 0.393
0.922HisLeu: 0.922 ± 0.369
0.0HisMet: 0.0 ± 0.0
1.959HisAsn: 1.959 ± 0.618
0.115HisPro: 0.115 ± 0.124
0.461HisGln: 0.461 ± 0.214
0.0HisArg: 0.0 ± 0.0
0.115HisSer: 0.115 ± 0.129
0.691HisThr: 0.691 ± 0.32
0.691HisVal: 0.691 ± 0.268
0.346HisTrp: 0.346 ± 0.285
0.691HisTyr: 0.691 ± 0.33
0.0HisXaa: 0.0 ± 0.0
Ile
4.263IleAla: 4.263 ± 0.781
0.346IleCys: 0.346 ± 0.201
4.608IleAsp: 4.608 ± 0.656
6.682IleGlu: 6.682 ± 1.06
2.419IlePhe: 2.419 ± 0.521
3.687IleGly: 3.687 ± 0.867
0.691IleHis: 0.691 ± 0.323
4.378IleIle: 4.378 ± 0.711
7.028IleLys: 7.028 ± 0.801
5.069IleLeu: 5.069 ± 1.144
1.843IleMet: 1.843 ± 0.445
5.069IleAsn: 5.069 ± 0.643
1.613IlePro: 1.613 ± 0.344
2.419IleGln: 2.419 ± 0.437
1.728IleArg: 1.728 ± 0.412
3.571IleSer: 3.571 ± 0.748
5.876IleThr: 5.876 ± 0.869
4.147IleVal: 4.147 ± 0.686
1.382IleTrp: 1.382 ± 0.521
3.226IleTyr: 3.226 ± 0.614
0.0IleXaa: 0.0 ± 0.0
Lys
6.797LysAla: 6.797 ± 1.11
1.037LysCys: 1.037 ± 0.517
5.069LysAsp: 5.069 ± 0.841
9.101LysGlu: 9.101 ± 1.661
2.419LysPhe: 2.419 ± 0.513
5.069LysGly: 5.069 ± 0.75
1.267LysHis: 1.267 ± 0.477
6.221LysIle: 6.221 ± 0.876
8.295LysLys: 8.295 ± 1.115
7.949LysLeu: 7.949 ± 1.082
2.765LysMet: 2.765 ± 0.448
5.645LysAsn: 5.645 ± 0.756
1.152LysPro: 1.152 ± 0.32
3.917LysGln: 3.917 ± 0.642
3.802LysArg: 3.802 ± 0.799
4.954LysSer: 4.954 ± 0.716
6.106LysThr: 6.106 ± 0.955
5.645LysVal: 5.645 ± 0.637
1.613LysTrp: 1.613 ± 0.386
3.571LysTyr: 3.571 ± 0.748
0.0LysXaa: 0.0 ± 0.0
Leu
5.645LeuAla: 5.645 ± 0.811
0.23LeuCys: 0.23 ± 0.18
4.608LeuAsp: 4.608 ± 0.744
6.106LeuGlu: 6.106 ± 1.104
4.032LeuPhe: 4.032 ± 0.799
4.839LeuGly: 4.839 ± 0.719
1.267LeuHis: 1.267 ± 0.427
7.028LeuIle: 7.028 ± 1.013
8.065LeuLys: 8.065 ± 0.797
5.76LeuLeu: 5.76 ± 1.159
1.382LeuMet: 1.382 ± 0.446
4.954LeuAsn: 4.954 ± 0.927
2.995LeuPro: 2.995 ± 0.576
3.226LeuGln: 3.226 ± 0.551
2.65LeuArg: 2.65 ± 0.55
5.184LeuSer: 5.184 ± 0.718
4.954LeuThr: 4.954 ± 0.841
5.645LeuVal: 5.645 ± 0.638
1.613LeuTrp: 1.613 ± 0.439
4.378LeuTyr: 4.378 ± 0.723
0.0LeuXaa: 0.0 ± 0.0
Met
2.765MetAla: 2.765 ± 0.515
0.115MetCys: 0.115 ± 0.104
1.267MetAsp: 1.267 ± 0.415
1.728MetGlu: 1.728 ± 0.621
0.691MetPhe: 0.691 ± 0.282
1.037MetGly: 1.037 ± 0.339
0.115MetHis: 0.115 ± 0.139
2.304MetIle: 2.304 ± 0.596
2.765MetLys: 2.765 ± 0.525
1.613MetLeu: 1.613 ± 0.548
0.23MetMet: 0.23 ± 0.175
1.843MetAsn: 1.843 ± 0.415
0.23MetPro: 0.23 ± 0.152
1.382MetGln: 1.382 ± 0.392
0.576MetArg: 0.576 ± 0.275
1.152MetSer: 1.152 ± 0.329
1.728MetThr: 1.728 ± 0.488
1.382MetVal: 1.382 ± 0.284
0.23MetTrp: 0.23 ± 0.159
1.152MetTyr: 1.152 ± 0.383
0.0MetXaa: 0.0 ± 0.0
Asn
5.3AsnAla: 5.3 ± 1.103
0.115AsnCys: 0.115 ± 0.129
4.263AsnAsp: 4.263 ± 0.782
5.184AsnGlu: 5.184 ± 0.88
2.189AsnPhe: 2.189 ± 0.531
6.336AsnGly: 6.336 ± 0.914
0.691AsnHis: 0.691 ± 0.292
3.571AsnIle: 3.571 ± 0.665
5.991AsnLys: 5.991 ± 1.255
5.184AsnLeu: 5.184 ± 0.768
1.152AsnMet: 1.152 ± 0.405
2.765AsnAsn: 2.765 ± 0.622
2.074AsnPro: 2.074 ± 0.481
2.189AsnGln: 2.189 ± 0.467
2.074AsnArg: 2.074 ± 0.381
5.184AsnSer: 5.184 ± 0.693
4.954AsnThr: 4.954 ± 0.872
3.687AsnVal: 3.687 ± 0.634
1.037AsnTrp: 1.037 ± 0.439
2.419AsnTyr: 2.419 ± 0.688
0.0AsnXaa: 0.0 ± 0.0
Pro
1.152ProAla: 1.152 ± 0.379
0.115ProCys: 0.115 ± 0.109
1.613ProAsp: 1.613 ± 0.46
1.498ProGlu: 1.498 ± 0.345
1.037ProPhe: 1.037 ± 0.312
0.346ProGly: 0.346 ± 0.263
0.115ProHis: 0.115 ± 0.125
1.959ProIle: 1.959 ± 0.567
2.074ProLys: 2.074 ± 0.603
2.074ProLeu: 2.074 ± 0.476
0.691ProMet: 0.691 ± 0.243
2.189ProAsn: 2.189 ± 0.743
0.576ProPro: 0.576 ± 0.274
0.576ProGln: 0.576 ± 0.239
0.346ProArg: 0.346 ± 0.161
1.498ProSer: 1.498 ± 0.461
2.189ProThr: 2.189 ± 0.5
1.382ProVal: 1.382 ± 0.438
0.23ProTrp: 0.23 ± 0.151
0.576ProTyr: 0.576 ± 0.238
0.0ProXaa: 0.0 ± 0.0
Gln
3.456GlnAla: 3.456 ± 0.784
0.346GlnCys: 0.346 ± 0.21
2.074GlnAsp: 2.074 ± 0.499
2.88GlnGlu: 2.88 ± 0.831
1.728GlnPhe: 1.728 ± 0.445
2.535GlnGly: 2.535 ± 0.538
0.461GlnHis: 0.461 ± 0.237
1.843GlnIle: 1.843 ± 0.403
2.765GlnLys: 2.765 ± 0.626
3.341GlnLeu: 3.341 ± 0.578
1.037GlnMet: 1.037 ± 0.278
2.88GlnAsn: 2.88 ± 0.483
1.382GlnPro: 1.382 ± 0.414
1.728GlnGln: 1.728 ± 0.424
1.152GlnArg: 1.152 ± 0.36
2.419GlnSer: 2.419 ± 0.549
2.074GlnThr: 2.074 ± 0.432
2.304GlnVal: 2.304 ± 0.677
0.691GlnTrp: 0.691 ± 0.248
0.922GlnTyr: 0.922 ± 0.286
0.0GlnXaa: 0.0 ± 0.0
Arg
2.189ArgAla: 2.189 ± 0.67
0.461ArgCys: 0.461 ± 0.207
1.843ArgAsp: 1.843 ± 0.47
2.419ArgGlu: 2.419 ± 0.615
0.691ArgPhe: 0.691 ± 0.255
1.843ArgGly: 1.843 ± 0.363
0.691ArgHis: 0.691 ± 0.269
2.189ArgIle: 2.189 ± 0.441
4.378ArgLys: 4.378 ± 0.966
3.917ArgLeu: 3.917 ± 0.658
0.461ArgMet: 0.461 ± 0.24
2.304ArgAsn: 2.304 ± 0.619
0.576ArgPro: 0.576 ± 0.259
1.728ArgGln: 1.728 ± 0.424
1.613ArgArg: 1.613 ± 0.44
1.267ArgSer: 1.267 ± 0.433
1.498ArgThr: 1.498 ± 0.384
1.613ArgVal: 1.613 ± 0.414
0.115ArgTrp: 0.115 ± 0.099
2.189ArgTyr: 2.189 ± 0.525
0.0ArgXaa: 0.0 ± 0.0
Ser
5.645SerAla: 5.645 ± 1.777
0.806SerCys: 0.806 ± 0.37
4.147SerAsp: 4.147 ± 0.714
3.917SerGlu: 3.917 ± 0.687
2.65SerPhe: 2.65 ± 0.496
5.53SerGly: 5.53 ± 1.821
0.691SerHis: 0.691 ± 0.283
3.687SerIle: 3.687 ± 0.757
5.3SerLys: 5.3 ± 0.746
4.954SerLeu: 4.954 ± 0.762
1.843SerMet: 1.843 ± 0.363
3.571SerAsn: 3.571 ± 0.698
0.922SerPro: 0.922 ± 0.288
2.535SerGln: 2.535 ± 0.569
2.765SerArg: 2.765 ± 0.381
5.645SerSer: 5.645 ± 1.041
3.571SerThr: 3.571 ± 0.754
3.917SerVal: 3.917 ± 0.889
0.922SerTrp: 0.922 ± 0.349
1.959SerTyr: 1.959 ± 0.428
0.0SerXaa: 0.0 ± 0.0
Thr
3.917ThrAla: 3.917 ± 0.635
0.23ThrCys: 0.23 ± 0.164
3.341ThrAsp: 3.341 ± 0.753
5.876ThrGlu: 5.876 ± 0.785
2.765ThrPhe: 2.765 ± 0.563
4.032ThrGly: 4.032 ± 0.569
0.346ThrHis: 0.346 ± 0.208
4.493ThrIle: 4.493 ± 0.948
5.76ThrLys: 5.76 ± 0.63
5.876ThrLeu: 5.876 ± 0.75
1.382ThrMet: 1.382 ± 0.419
4.608ThrAsn: 4.608 ± 0.752
2.419ThrPro: 2.419 ± 0.469
2.65ThrGln: 2.65 ± 0.579
1.959ThrArg: 1.959 ± 0.482
4.839ThrSer: 4.839 ± 0.608
3.802ThrThr: 3.802 ± 0.635
3.802ThrVal: 3.802 ± 0.787
1.037ThrTrp: 1.037 ± 0.299
1.843ThrTyr: 1.843 ± 0.446
0.0ThrXaa: 0.0 ± 0.0
Val
4.032ValAla: 4.032 ± 0.795
0.576ValCys: 0.576 ± 0.252
4.032ValAsp: 4.032 ± 0.618
5.069ValGlu: 5.069 ± 0.611
3.226ValPhe: 3.226 ± 0.759
4.263ValGly: 4.263 ± 0.791
0.691ValHis: 0.691 ± 0.285
3.802ValIle: 3.802 ± 0.653
5.3ValLys: 5.3 ± 0.707
2.765ValLeu: 2.765 ± 0.512
1.843ValMet: 1.843 ± 0.439
2.65ValAsn: 2.65 ± 0.571
1.728ValPro: 1.728 ± 0.426
1.843ValGln: 1.843 ± 0.502
2.765ValArg: 2.765 ± 0.784
5.76ValSer: 5.76 ± 1.477
4.839ValThr: 4.839 ± 0.772
3.226ValVal: 3.226 ± 0.715
0.691ValTrp: 0.691 ± 0.263
2.535ValTyr: 2.535 ± 0.447
0.0ValXaa: 0.0 ± 0.0
Trp
0.576TrpAla: 0.576 ± 0.244
0.23TrpCys: 0.23 ± 0.182
0.691TrpAsp: 0.691 ± 0.317
0.806TrpGlu: 0.806 ± 0.316
0.922TrpPhe: 0.922 ± 0.371
0.806TrpGly: 0.806 ± 0.378
0.461TrpHis: 0.461 ± 0.243
0.576TrpIle: 0.576 ± 0.22
0.922TrpLys: 0.922 ± 0.315
1.843TrpLeu: 1.843 ± 0.587
0.461TrpMet: 0.461 ± 0.259
1.728TrpAsn: 1.728 ± 0.49
0.0TrpPro: 0.0 ± 0.0
0.922TrpGln: 0.922 ± 0.325
0.346TrpArg: 0.346 ± 0.266
1.613TrpSer: 1.613 ± 0.34
0.806TrpThr: 0.806 ± 0.327
0.23TrpVal: 0.23 ± 0.176
0.23TrpTrp: 0.23 ± 0.149
1.267TrpTyr: 1.267 ± 0.407
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.382TyrAla: 1.382 ± 0.388
0.691TyrCys: 0.691 ± 0.326
2.304TyrAsp: 2.304 ± 0.716
3.917TyrGlu: 3.917 ± 0.763
2.535TyrPhe: 2.535 ± 0.378
2.535TyrGly: 2.535 ± 0.521
0.806TyrHis: 0.806 ± 0.267
3.687TyrIle: 3.687 ± 0.844
2.765TyrLys: 2.765 ± 0.743
3.456TyrLeu: 3.456 ± 0.68
0.806TyrMet: 0.806 ± 0.352
3.802TyrAsn: 3.802 ± 0.505
1.037TyrPro: 1.037 ± 0.382
1.382TyrGln: 1.382 ± 0.512
1.382TyrArg: 1.382 ± 0.478
1.728TyrSer: 1.728 ± 0.347
2.88TyrThr: 2.88 ± 0.646
2.189TyrVal: 2.189 ± 0.55
0.23TyrTrp: 0.23 ± 0.19
2.419TyrTyr: 2.419 ± 0.693
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (8681 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski