Simian foamy virus
Average proteome isoelectric point is 7.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K7YNA6|K7YNA6_9RETR Pr125Pol OS=Simian foamy virus OX=11642 GN=pol PE=4 SV=1
MM1 pKa = 7.74 DD2 pKa = 4.5 TYY4 pKa = 11.16 QEE6 pKa = 4.26 EE7 pKa = 4.39 EE8 pKa = 4.68 SVASTSGVQDD18 pKa = 3.74 LQTLSEE24 pKa = 4.24 LVGPEE29 pKa = 3.58 AAGEE33 pKa = 4.45 GEE35 pKa = 4.49 DD36 pKa = 3.77 TLSDD40 pKa = 3.58 TEE42 pKa = 4.35 EE43 pKa = 3.84 VSRR46 pKa = 11.84 RR47 pKa = 11.84 SRR49 pKa = 11.84 KK50 pKa = 6.03 NTKK53 pKa = 9.9 RR54 pKa = 11.84 GAKK57 pKa = 9.22 MITFHH62 pKa = 6.89 AYY64 pKa = 9.67 KK65 pKa = 10.14 EE66 pKa = 4.19 IEE68 pKa = 4.38 EE69 pKa = 4.67 KK70 pKa = 11.0 NPQNLKK76 pKa = 8.67 LHH78 pKa = 5.78 NWIPTPEE85 pKa = 4.05 EE86 pKa = 3.8 MSKK89 pKa = 10.65 SICSKK94 pKa = 10.71 FILHH98 pKa = 6.8 GLYY101 pKa = 10.31 SAEE104 pKa = 3.99 KK105 pKa = 10.17 VIEE108 pKa = 4.01 ILNMPFEE115 pKa = 4.58 ISWDD119 pKa = 3.6 QSEE122 pKa = 4.6 TNPKK126 pKa = 9.93 CFIVSYY132 pKa = 9.15 TCIYY136 pKa = 9.82 CSAIIHH142 pKa = 6.24 EE143 pKa = 4.74 PMPVTYY149 pKa = 10.5 NADD152 pKa = 3.17 VGVWVKK158 pKa = 10.45 LNPLRR163 pKa = 11.84 GVVGSAVFMMKK174 pKa = 9.75 KK175 pKa = 9.71 HH176 pKa = 6.05 EE177 pKa = 4.55 KK178 pKa = 8.84 NCCFIKK184 pKa = 10.42 PSPSKK189 pKa = 10.88 QEE191 pKa = 3.98 GPKK194 pKa = 9.77 PRR196 pKa = 11.84 PRR198 pKa = 11.84 HH199 pKa = 6.0 DD200 pKa = 3.14 PVLRR204 pKa = 11.84 CKK206 pKa = 10.66 AFEE209 pKa = 4.19 RR210 pKa = 11.84 NYY212 pKa = 10.47 KK213 pKa = 9.62 PRR215 pKa = 11.84 PKK217 pKa = 10.02 RR218 pKa = 11.84 PRR220 pKa = 11.84 PGPINNEE227 pKa = 3.9 PCASSSDD234 pKa = 3.83 PLAVQPGSLCTDD246 pKa = 4.16 PVWDD250 pKa = 4.35 PAAILQVLSEE260 pKa = 4.09 QRR262 pKa = 11.84 EE263 pKa = 4.22 DD264 pKa = 3.69 SQSLAIHH271 pKa = 6.11 MSGGPFWEE279 pKa = 4.21 EE280 pKa = 4.4 VYY282 pKa = 10.84 GDD284 pKa = 4.37 SYY286 pKa = 10.61 MGPPTRR292 pKa = 11.84 PGDD295 pKa = 3.7 HH296 pKa = 6.23 TVLL299 pKa = 3.97
Molecular weight: 33.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.551
IPC2_protein 5.601
IPC_protein 5.588
Toseland 5.855
ProMoST 5.881
Dawson 5.779
Bjellqvist 5.817
Wikipedia 5.753
Rodwell 5.753
Grimsley 5.931
Solomon 5.779
Lehninger 5.766
Nozaki 6.02
DTASelect 6.186
Thurlkill 6.148
EMBOSS 6.109
Sillero 6.109
Patrickios 4.05
IPC_peptide 5.792
IPC2_peptide 6.109
IPC2.peptide.svr19 6.13
Protein with the highest isoelectric point:
>tr|K7Z2A0|K7Z2A0_9RETR Gag polyprotein OS=Simian foamy virus OX=11642 GN=gag PE=4 SV=1
MM1 pKa = 7.58 NPLQLLQPLQAEE13 pKa = 4.58 VKK15 pKa = 9.03 GNKK18 pKa = 9.55 LIAHH22 pKa = 7.1 WDD24 pKa = 3.32 SGASITCIPEE34 pKa = 3.83 SFLEE38 pKa = 4.23 EE39 pKa = 3.66 EE40 pKa = 4.48 TPIKK44 pKa = 9.66 KK45 pKa = 8.81 TIIKK49 pKa = 8.36 TIHH52 pKa = 5.28 GQKK55 pKa = 8.18 EE56 pKa = 3.93 QKK58 pKa = 10.41 VYY60 pKa = 11.34 YY61 pKa = 9.47 LTFKK65 pKa = 10.75 VNGRR69 pKa = 11.84 KK70 pKa = 9.15 VEE72 pKa = 4.14 AEE74 pKa = 4.44 VIASPYY80 pKa = 10.8 DD81 pKa = 3.83 YY82 pKa = 10.89 ILLSPTDD89 pKa = 4.01 VPWLVQKK96 pKa = 10.19 PLQLTILVPLQDD108 pKa = 3.74 YY109 pKa = 9.76 QSRR112 pKa = 11.84 ILEE115 pKa = 4.13 KK116 pKa = 9.68 TALPEE121 pKa = 3.92 EE122 pKa = 4.71 FKK124 pKa = 11.13 KK125 pKa = 10.53 QLQTLFLKK133 pKa = 10.71 YY134 pKa = 10.67 DD135 pKa = 3.76 NLWQHH140 pKa = 6.27 WEE142 pKa = 3.98 NQVGHH147 pKa = 6.44 RR148 pKa = 11.84 KK149 pKa = 8.88 IRR151 pKa = 11.84 PHH153 pKa = 6.76 NIATGDD159 pKa = 3.71 YY160 pKa = 9.61 PPRR163 pKa = 11.84 PQKK166 pKa = 10.35 QYY168 pKa = 10.54 PINPKK173 pKa = 9.76 ARR175 pKa = 11.84 SSIQVVIDD183 pKa = 4.03 DD184 pKa = 4.51 LLKK187 pKa = 10.94 QGVLVQQNSTMNTPVYY203 pKa = 9.51 PVPKK207 pKa = 9.66 PDD209 pKa = 3.26 GRR211 pKa = 11.84 WRR213 pKa = 11.84 MVLDD217 pKa = 3.37 YY218 pKa = 11.29 RR219 pKa = 11.84 EE220 pKa = 4.13 VNKK223 pKa = 9.58 TIPLIAAQNQHH234 pKa = 5.64 SAGILATIVRR244 pKa = 11.84 KK245 pKa = 9.84 KK246 pKa = 11.06 YY247 pKa = 9.06 KK248 pKa = 8.27 TTLDD252 pKa = 3.57 LANGFWAHH260 pKa = 7.43 PITPEE265 pKa = 4.44 SYY267 pKa = 9.5 WLTAFTWQGKK277 pKa = 6.31 QYY279 pKa = 10.3 CWTRR283 pKa = 11.84 LPQGFLNSPALFTADD298 pKa = 3.46 VVDD301 pKa = 4.32 LLKK304 pKa = 10.84 EE305 pKa = 3.81 ISNVQAYY312 pKa = 10.42 VDD314 pKa = 4.91 DD315 pKa = 5.2 IYY317 pKa = 11.3 LSHH320 pKa = 8.21 DD321 pKa = 4.13 DD322 pKa = 3.97 PQEE325 pKa = 4.03 HH326 pKa = 6.83 LNQLEE331 pKa = 4.31 KK332 pKa = 11.01 VFQILLQAGYY342 pKa = 10.34 VVSLKK347 pKa = 10.54 KK348 pKa = 10.59 SEE350 pKa = 4.12 IAQKK354 pKa = 9.0 TVEE357 pKa = 4.04 FLGFNITKK365 pKa = 9.78 EE366 pKa = 4.22 GRR368 pKa = 11.84 GLTEE372 pKa = 3.8 AFKK375 pKa = 11.17 AKK377 pKa = 10.37 LLDD380 pKa = 3.27 ITPPKK385 pKa = 10.15 DD386 pKa = 3.48 LKK388 pKa = 10.77 QLQSILGLLNFARR401 pKa = 11.84 NFILNFAEE409 pKa = 4.49 LVKK412 pKa = 10.28 PLYY415 pKa = 10.76 SLISSAKK422 pKa = 8.61 GKK424 pKa = 10.29 YY425 pKa = 9.43 IEE427 pKa = 4.27 WSNEE431 pKa = 3.35 NTVQLQTIIKK441 pKa = 9.62 ALNDD445 pKa = 3.51 ADD447 pKa = 3.95 NLEE450 pKa = 3.86 EE451 pKa = 4.93 RR452 pKa = 11.84 IPEE455 pKa = 3.62 KK456 pKa = 10.77 RR457 pKa = 11.84 LIIKK461 pKa = 10.12 VNTSPSAGYY470 pKa = 9.09 VRR472 pKa = 11.84 YY473 pKa = 10.45 YY474 pKa = 11.15 NEE476 pKa = 4.16 TGKK479 pKa = 10.81 KK480 pKa = 9.46 PIMYY484 pKa = 9.87 LNYY487 pKa = 9.51 VFSKK491 pKa = 11.38 AEE493 pKa = 3.87 LKK495 pKa = 9.27 FTLLEE500 pKa = 4.21 KK501 pKa = 10.93 LLTTMHH507 pKa = 6.98 KK508 pKa = 10.82 ALIKK512 pKa = 11.07 AMDD515 pKa = 3.95 LAMGQEE521 pKa = 3.94 ILVYY525 pKa = 10.3 SPVVSMTKK533 pKa = 9.45 IQKK536 pKa = 8.43 TPIPEE541 pKa = 4.03 RR542 pKa = 11.84 KK543 pKa = 9.23 ALPIRR548 pKa = 11.84 WITWMTYY555 pKa = 10.7 LEE557 pKa = 4.43 DD558 pKa = 3.7 PRR560 pKa = 11.84 IQFHH564 pKa = 5.99 YY565 pKa = 10.92 DD566 pKa = 2.7 KK567 pKa = 10.67 TLPEE571 pKa = 4.72 LKK573 pKa = 10.38 NIPDD577 pKa = 3.61 VLTEE581 pKa = 3.97 NSSKK585 pKa = 10.89 IMIHH589 pKa = 6.1 PSQYY593 pKa = 10.99 NSVFYY598 pKa = 10.53 TDD600 pKa = 3.69 GSAIRR605 pKa = 11.84 SPDD608 pKa = 3.09 PTKK611 pKa = 10.91 SHH613 pKa = 6.21 NAGMGIVQAKK623 pKa = 8.7 FSPEE627 pKa = 3.79 LQVINQWSIPLGNHH641 pKa = 5.23 TAQMAEE647 pKa = 3.6 IAAVEE652 pKa = 4.32 FACKK656 pKa = 9.73 KK657 pKa = 9.7 ALKK660 pKa = 8.67 ITGPILIITDD670 pKa = 3.6 SFYY673 pKa = 11.35 VAEE676 pKa = 4.48 SANKK680 pKa = 8.91 EE681 pKa = 3.85 LPYY684 pKa = 9.92 WKK686 pKa = 10.68 SNGFVNNKK694 pKa = 9.3 KK695 pKa = 10.41 KK696 pKa = 10.05 PLKK699 pKa = 10.29 HH700 pKa = 5.03 VSKK703 pKa = 9.37 WKK705 pKa = 10.68 SIAEE709 pKa = 4.16 CLSLKK714 pKa = 10.35 PDD716 pKa = 2.92 ITIQHH721 pKa = 5.9 EE722 pKa = 4.27 RR723 pKa = 11.84 GHH725 pKa = 5.48 QPIYY729 pKa = 10.56 TSIHH733 pKa = 5.42 TEE735 pKa = 4.02 GNALADD741 pKa = 3.72 KK742 pKa = 10.67 LATQGSYY749 pKa = 9.19 VVNNNDD755 pKa = 4.09 KK756 pKa = 10.94 KK757 pKa = 10.69 PNLDD761 pKa = 3.68 AEE763 pKa = 4.7 LDD765 pKa = 3.58 HH766 pKa = 7.08 LIQGKK771 pKa = 8.1 YY772 pKa = 8.64 PKK774 pKa = 9.73 GYY776 pKa = 8.36 PKK778 pKa = 10.2 QYY780 pKa = 9.44 PYY782 pKa = 11.62 YY783 pKa = 9.86 MEE785 pKa = 5.19 DD786 pKa = 2.9 GKK788 pKa = 11.64 VKK790 pKa = 10.32 VNRR793 pKa = 11.84 PEE795 pKa = 3.86 GTKK798 pKa = 9.91 IIPPSLEE805 pKa = 3.54 RR806 pKa = 11.84 AGIVQKK812 pKa = 10.91 AHH814 pKa = 6.18 NLAHH818 pKa = 6.88 TGRR821 pKa = 11.84 EE822 pKa = 3.94 ATLLKK827 pKa = 10.2 IANLYY832 pKa = 6.99 WWPNMRR838 pKa = 11.84 KK839 pKa = 9.79 DD840 pKa = 3.63 VVKK843 pKa = 10.73 QLGRR847 pKa = 11.84 CQQCLVTNAFNQTSGPILRR866 pKa = 11.84 PTRR869 pKa = 11.84 PLKK872 pKa = 10.76 PFDD875 pKa = 4.3 KK876 pKa = 10.94 FFIDD880 pKa = 4.29 YY881 pKa = 10.24 IGPLPPSNGYY891 pKa = 8.92 LHH893 pKa = 6.42 VLVVVDD899 pKa = 4.43 SMTGFTWLYY908 pKa = 5.79 PTKK911 pKa = 10.77 APTTNATVKK920 pKa = 10.39 ALNVLTSIAVPKK932 pKa = 10.18 VIHH935 pKa = 6.68 SDD937 pKa = 2.8 QGAAFTSSTFADD949 pKa = 3.46 WAKK952 pKa = 10.69 EE953 pKa = 3.63 RR954 pKa = 11.84 GIQLEE959 pKa = 4.34 FSTPYY964 pKa = 10.25 HH965 pKa = 5.98 PQSSGKK971 pKa = 8.3 VEE973 pKa = 4.3 RR974 pKa = 11.84 KK975 pKa = 10.0 NSDD978 pKa = 2.61 IKK980 pKa = 11.08 RR981 pKa = 11.84 LLTKK985 pKa = 10.58 LLVGRR990 pKa = 11.84 PTKK993 pKa = 10.03 WYY995 pKa = 9.91 DD996 pKa = 3.56 LLPVVQLALNNSYY1009 pKa = 10.92 SPSLKK1014 pKa = 9.01 HH1015 pKa = 6.12 TPHH1018 pKa = 6.28 QLLFGIDD1025 pKa = 3.66 SNTPFANQDD1034 pKa = 3.49 TLDD1037 pKa = 3.66 LTRR1040 pKa = 11.84 EE1041 pKa = 4.18 EE1042 pKa = 4.48 EE1043 pKa = 4.13 LSLLQEE1049 pKa = 4.45 IRR1051 pKa = 11.84 SSLHH1055 pKa = 5.69 QPSTPPASTRR1065 pKa = 11.84 SWSPIVGQIVQEE1077 pKa = 4.17 RR1078 pKa = 11.84 VPRR1081 pKa = 11.84 PASLRR1086 pKa = 11.84 PRR1088 pKa = 11.84 WHH1090 pKa = 6.07 KK1091 pKa = 9.93 PSRR1094 pKa = 11.84 IVDD1097 pKa = 3.34 ILNEE1101 pKa = 4.07 RR1102 pKa = 11.84 TVVIVDD1108 pKa = 3.11 HH1109 pKa = 6.75 LGNNRR1114 pKa = 11.84 TVSIDD1119 pKa = 3.5 NLKK1122 pKa = 8.92 LTPHH1126 pKa = 6.48 QDD1128 pKa = 2.79 GTSNVSSTMDD1138 pKa = 3.22 HH1139 pKa = 7.04 LEE1141 pKa = 3.79
Molecular weight: 129.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.709
IPC2_protein 8.697
IPC_protein 8.536
Toseland 9.311
ProMoST 9.092
Dawson 9.604
Bjellqvist 9.297
Wikipedia 9.765
Rodwell 9.897
Grimsley 9.677
Solomon 9.619
Lehninger 9.575
Nozaki 9.37
DTASelect 9.282
Thurlkill 9.443
EMBOSS 9.75
Sillero 9.531
Patrickios 4.8
IPC_peptide 9.604
IPC2_peptide 7.775
IPC2.peptide.svr19 7.74
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3553
299
1141
710.6
80.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.685 ± 0.185
1.379 ± 0.483
4.391 ± 0.21
6.023 ± 0.577
2.702 ± 0.176
5.573 ± 0.951
2.477 ± 0.236
6.642 ± 0.632
5.826 ± 1.276
9.063 ± 0.879
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.97 ± 0.234
5.094 ± 0.215
7.233 ± 0.839
5.77 ± 0.52
5.207 ± 0.939
6.98 ± 0.605
5.995 ± 0.28
6.164 ± 0.14
1.998 ± 0.272
3.828 ± 0.41
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here