Rhinolophus bat coronavirus HKU2

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Rhinacovirus

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8JNZ7|A8JNZ7_9ALPC Uncharacterized protein NS7a OS=Rhinolophus bat coronavirus HKU2 OX=693998 GN=NS7a PE=4 SV=1
MM1 pKa = 6.9NQLIFFLCMMCCCAILFDD19 pKa = 3.92WLFNLFFYY27 pKa = 10.66ACQVDD32 pKa = 3.77TWQEE36 pKa = 3.75FAFSCNWSWSLFLEE50 pKa = 5.61DD51 pKa = 4.06FSTWFKK57 pKa = 10.68CLSVVLIGIFSAASLMFVDD76 pKa = 5.48FAVEE80 pKa = 3.99VFDD83 pKa = 5.11LFEE86 pKa = 4.78HH87 pKa = 6.35FFINIGKK94 pKa = 9.15FCRR97 pKa = 11.84FVV99 pKa = 2.94

Molecular weight: 11.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8JNZ7|A8JNZ7_9ALPC Uncharacterized protein NS7a OS=Rhinolophus bat coronavirus HKU2 OX=693998 GN=NS7a PE=4 SV=1
MM1 pKa = 7.27ATVNWGDD8 pKa = 3.3AVEE11 pKa = 4.15QAEE14 pKa = 4.31SRR16 pKa = 11.84GRR18 pKa = 11.84KK19 pKa = 9.27RR20 pKa = 11.84IPLSLFAPLRR30 pKa = 11.84VTDD33 pKa = 5.74GKK35 pKa = 10.65NFWNVMPRR43 pKa = 11.84NGVPTGRR50 pKa = 11.84GNPDD54 pKa = 2.91QQIGYY59 pKa = 8.74WVEE62 pKa = 3.8QKK64 pKa = 9.42RR65 pKa = 11.84WRR67 pKa = 11.84MQKK70 pKa = 9.23GQRR73 pKa = 11.84KK74 pKa = 8.2DD75 pKa = 3.49QPSNWHH81 pKa = 6.59FYY83 pKa = 10.41YY84 pKa = 10.82LGTGPHH90 pKa = 6.71ADD92 pKa = 2.98APFRR96 pKa = 11.84KK97 pKa = 9.62RR98 pKa = 11.84IQGVHH103 pKa = 4.57WVAVDD108 pKa = 3.84GAKK111 pKa = 9.74TSPTGLGVRR120 pKa = 11.84NRR122 pKa = 11.84NKK124 pKa = 10.5EE125 pKa = 3.53PATPQFGFQLPADD138 pKa = 4.02VTVVEE143 pKa = 4.37ATSRR147 pKa = 11.84SASRR151 pKa = 11.84SQSRR155 pKa = 11.84SRR157 pKa = 11.84NQSQSRR163 pKa = 11.84SGAQTPRR170 pKa = 11.84VQQSSQAVDD179 pKa = 3.09IVAAVKK185 pKa = 10.09QALADD190 pKa = 3.97LGIASSQSKK199 pKa = 9.3SQSGKK204 pKa = 7.97NTPKK208 pKa = 9.49PRR210 pKa = 11.84SRR212 pKa = 11.84AVSPAPAPKK221 pKa = 9.61PARR224 pKa = 11.84KK225 pKa = 9.63QMDD228 pKa = 3.32KK229 pKa = 11.02PEE231 pKa = 4.2WKK233 pKa = 9.76RR234 pKa = 11.84VPNSEE239 pKa = 3.83EE240 pKa = 3.9DD241 pKa = 3.4VRR243 pKa = 11.84KK244 pKa = 10.39CFGPRR249 pKa = 11.84SASRR253 pKa = 11.84NFGDD257 pKa = 4.14SDD259 pKa = 4.08LVQHH263 pKa = 6.07GVEE266 pKa = 4.22AKK268 pKa = 10.17HH269 pKa = 6.01FPTIAEE275 pKa = 4.36LLPTQAALAFGSEE288 pKa = 4.03ITTKK292 pKa = 10.34EE293 pKa = 3.54AGDD296 pKa = 3.87FVEE299 pKa = 4.54VTYY302 pKa = 10.44HH303 pKa = 5.7YY304 pKa = 11.21VMKK307 pKa = 10.7VSKK310 pKa = 9.41TDD312 pKa = 3.13KK313 pKa = 10.21NLPRR317 pKa = 11.84FLEE320 pKa = 4.03QVAAYY325 pKa = 8.91SKK327 pKa = 9.88PSQIRR332 pKa = 11.84RR333 pKa = 11.84SQSQQDD339 pKa = 4.0LNVDD343 pKa = 3.77APAFTPAPPATPVSRR358 pKa = 11.84NPDD361 pKa = 3.25FPEE364 pKa = 4.32EE365 pKa = 3.92EE366 pKa = 4.14VEE368 pKa = 4.46MVDD371 pKa = 5.13EE372 pKa = 5.29IINN375 pKa = 3.53

Molecular weight: 41.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

12921

75

6727

1615.1

179.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.182 ± 0.272

3.398 ± 0.419

6.029 ± 0.683

3.792 ± 0.165

6.215 ± 0.315

6.911 ± 0.281

1.865 ± 0.165

4.961 ± 0.222

5.805 ± 0.551

8.885 ± 0.386

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.989 ± 0.131

5.394 ± 0.202

3.235 ± 0.63

2.709 ± 0.607

3.506 ± 0.53

6.996 ± 0.283

5.626 ± 0.316

9.837 ± 0.462

1.3 ± 0.28

4.365 ± 0.318
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski