Circoviridae 3 LDMD-2013

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; environmental samples

Average proteome isoelectric point is 9.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5T672|S5T672_9CIRC Coat protein OS=Circoviridae 3 LDMD-2013 OX=1379707 PE=4 SV=1
MM1 pKa = 7.76SIPAQTWNTLGFGTRR16 pKa = 11.84ATAAEE21 pKa = 4.27ALAGDD26 pKa = 4.55ALIAAGPVALGAAALGGIAYY46 pKa = 9.9AGYY49 pKa = 9.66KK50 pKa = 9.61QMARR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84PRR58 pKa = 11.84SFPGPYY64 pKa = 9.07RR65 pKa = 11.84PKK67 pKa = 10.54KK68 pKa = 9.24RR69 pKa = 11.84GSYY72 pKa = 9.99GPRR75 pKa = 11.84SRR77 pKa = 11.84PPSRR81 pKa = 11.84RR82 pKa = 11.84YY83 pKa = 9.53RR84 pKa = 11.84LPNLRR89 pKa = 11.84IGGWLNRR96 pKa = 11.84EE97 pKa = 4.27LKK99 pKa = 10.77FKK101 pKa = 10.57DD102 pKa = 3.17VQRR105 pKa = 11.84NVSISTTIAGSEE117 pKa = 3.81IDD119 pKa = 3.6PDD121 pKa = 4.17GGVNCLNAISQGDD134 pKa = 4.02GEE136 pKa = 4.35EE137 pKa = 4.05QRR139 pKa = 11.84DD140 pKa = 3.63GRR142 pKa = 11.84GYY144 pKa = 10.81KK145 pKa = 8.4ITSVHH150 pKa = 5.39LRR152 pKa = 11.84GYY154 pKa = 10.55VLFAGQSGAGATSDD168 pKa = 3.05HH169 pKa = 5.97MRR171 pKa = 11.84IILLQDD177 pKa = 3.21TQTNGTQFNAEE188 pKa = 4.09DD189 pKa = 4.12VIDD192 pKa = 4.05NSSGVNEE199 pKa = 4.27LQTVAFRR206 pKa = 11.84NLEE209 pKa = 3.69NTNRR213 pKa = 11.84FKK215 pKa = 10.63ILKK218 pKa = 9.54DD219 pKa = 3.22IVVHH223 pKa = 6.86KK224 pKa = 7.98PTTGLAGNQANPGDD238 pKa = 4.14VEE240 pKa = 5.0SNSATMPIQMDD251 pKa = 3.81VNFKK255 pKa = 10.78YY256 pKa = 10.02PVNVLCTGTGGTVSNLTDD274 pKa = 3.3NSFHH278 pKa = 7.66LMAISVNSGNSFRR291 pKa = 11.84YY292 pKa = 8.12ICRR295 pKa = 11.84TRR297 pKa = 11.84FVGG300 pKa = 4.21

Molecular weight: 32.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5T672|S5T672_9CIRC Coat protein OS=Circoviridae 3 LDMD-2013 OX=1379707 PE=4 SV=1
MM1 pKa = 8.05PSQQSRR7 pKa = 11.84NWVFTWNNPPFQGTDD22 pKa = 3.35LEE24 pKa = 4.44IEE26 pKa = 4.25EE27 pKa = 4.8KK28 pKa = 10.91LDD30 pKa = 4.43DD31 pKa = 4.37FFTGQEE37 pKa = 3.96RR38 pKa = 11.84LGDD41 pKa = 3.51WSYY44 pKa = 11.83LSWQLEE50 pKa = 4.1RR51 pKa = 11.84GEE53 pKa = 4.63SGTLHH58 pKa = 5.05VQAYY62 pKa = 7.73VQFAGNKK69 pKa = 8.86RR70 pKa = 11.84LASLRR75 pKa = 11.84ALWPGCHH82 pKa = 6.21AEE84 pKa = 4.12VRR86 pKa = 11.84RR87 pKa = 11.84GSHH90 pKa = 4.89EE91 pKa = 3.84QAVEE95 pKa = 4.03YY96 pKa = 9.9TSKK99 pKa = 10.91EE100 pKa = 3.8EE101 pKa = 3.85SRR103 pKa = 11.84IDD105 pKa = 3.41GGRR108 pKa = 11.84HH109 pKa = 5.4HH110 pKa = 6.65EE111 pKa = 4.33HH112 pKa = 6.47GEE114 pKa = 3.69ARR116 pKa = 11.84NRR118 pKa = 11.84PGRR121 pKa = 11.84RR122 pKa = 11.84RR123 pKa = 11.84DD124 pKa = 3.57LEE126 pKa = 4.3AMPGNLVAQKK136 pKa = 10.75LSGFPSPTVSVPFGMVTMVKK156 pKa = 10.13RR157 pKa = 11.84LLSLMSSMGGSHH169 pKa = 6.16MAFCADD175 pKa = 3.82CWTAIPSVWRR185 pKa = 11.84RR186 pKa = 11.84KK187 pKa = 10.68DD188 pKa = 3.27PVFLFWRR195 pKa = 11.84SVLSSLPTSVLHH207 pKa = 7.24DD208 pKa = 3.24GTGRR212 pKa = 11.84VFVLFVAVLVVNSVRR227 pKa = 11.84CGRR230 pKa = 11.84RR231 pKa = 11.84RR232 pKa = 11.84LWKK235 pKa = 8.91XII237 pKa = 3.74

Molecular weight: 26.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

537

237

300

268.5

29.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.449 ± 1.109

1.304 ± 0.217

4.283 ± 0.274

4.283 ± 0.917

4.283 ± 0.441

10.056 ± 0.913

2.235 ± 0.645

3.911 ± 1.256

3.166 ± 0.12

8.007 ± 0.72

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.421 ± 0.301

5.4 ± 1.382

5.028 ± 0.02

3.911 ± 0.064

8.752 ± 0.538

8.007 ± 0.72

5.773 ± 0.878

7.263 ± 0.903

2.048 ± 0.988

2.235 ± 0.547
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski