Stemphylium lycopersici
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9079 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A364N1R0|A0A364N1R0_9PLEO 3'(2') 5'-bisphosphate nucleotidase OS=Stemphylium lycopersici OX=183478 GN=DDE83_005700 PE=3 SV=1
MM1 pKa = 7.56 GFLSEE6 pKa = 5.51 LISDD10 pKa = 4.66 FKK12 pKa = 11.33 GSSSSGQGDD21 pKa = 3.45 QQQYY25 pKa = 7.46 QQQQSGYY32 pKa = 8.11 SQYY35 pKa = 10.82 PPQQHH40 pKa = 6.18 SPQPPPVSPPWYY52 pKa = 10.12 AEE54 pKa = 3.24 WDD56 pKa = 3.64 SRR58 pKa = 11.84 DD59 pKa = 3.57 NRR61 pKa = 11.84 WFFVNQHH68 pKa = 4.82 TGEE71 pKa = 3.89 RR72 pKa = 11.84 TYY74 pKa = 11.12 NYY76 pKa = 8.05 PGPGYY81 pKa = 10.39 AQQQGDD87 pKa = 4.14 YY88 pKa = 10.36 GAPPPQGGYY97 pKa = 10.98 GYY99 pKa = 10.91 GQGGYY104 pKa = 9.44 SNQGAYY110 pKa = 8.34 EE111 pKa = 3.94 QSAYY115 pKa = 10.9 DD116 pKa = 4.09 PTRR119 pKa = 11.84 SAQPQEE125 pKa = 4.31 EE126 pKa = 4.35 KK127 pKa = 10.89 SGGNGWKK134 pKa = 10.01 YY135 pKa = 10.66 AAAGAAGLAGGALLMHH151 pKa = 7.09 EE152 pKa = 4.55 SDD154 pKa = 4.96 KK155 pKa = 11.49 IEE157 pKa = 5.32 DD158 pKa = 3.72 GWDD161 pKa = 3.28 SAKK164 pKa = 10.81 QNISNGVDD172 pKa = 3.01 EE173 pKa = 5.35 FGNDD177 pKa = 2.95 VSNFPEE183 pKa = 4.46 NAAGWTGEE191 pKa = 3.98 QVGRR195 pKa = 11.84 AEE197 pKa = 4.13 NFGDD201 pKa = 4.81 DD202 pKa = 5.56 VEE204 pKa = 4.72 QKK206 pKa = 10.13 WDD208 pKa = 3.9 DD209 pKa = 3.58 GVQDD213 pKa = 4.27 VEE215 pKa = 5.22 DD216 pKa = 4.61 FPEE219 pKa = 4.75 NAAEE223 pKa = 4.01 WTGEE227 pKa = 4.0 KK228 pKa = 10.02 VQEE231 pKa = 4.45 VEE233 pKa = 6.29 DD234 pKa = 3.98 IPQDD238 pKa = 4.93 IEE240 pKa = 4.54 NKK242 pKa = 7.83 WDD244 pKa = 3.4 NMTEE248 pKa = 4.27 GIEE251 pKa = 4.44 QFGDD255 pKa = 3.8 DD256 pKa = 4.41 MGDD259 pKa = 3.29 AYY261 pKa = 11.01 DD262 pKa = 4.0 EE263 pKa = 4.69 GRR265 pKa = 11.84 DD266 pKa = 3.59 EE267 pKa = 5.28 QRR269 pKa = 11.84 YY270 pKa = 8.89 EE271 pKa = 4.05 DD272 pKa = 5.21 DD273 pKa = 3.71 YY274 pKa = 12.24
Molecular weight: 30.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.706
IPC2_protein 3.834
IPC_protein 3.834
Toseland 3.617
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.719
Rodwell 3.656
Grimsley 3.528
Solomon 3.808
Lehninger 3.757
Nozaki 3.923
DTASelect 4.126
Thurlkill 3.656
EMBOSS 3.732
Sillero 3.948
Patrickios 1.252
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.85
Protein with the highest isoelectric point:
>tr|A0A364N615|A0A364N615_9PLEO Replication factor A protein 3 OS=Stemphylium lycopersici OX=183478 GN=DDE83_003943 PE=3 SV=1
MM1 pKa = 7.54 PLARR5 pKa = 11.84 RR6 pKa = 11.84 HH7 pKa = 5.57 APRR10 pKa = 11.84 TARR13 pKa = 11.84 ATRR16 pKa = 11.84 TTARR20 pKa = 11.84 PSLKK24 pKa = 8.96 TRR26 pKa = 11.84 LMGGRR31 pKa = 11.84 KK32 pKa = 7.3 TRR34 pKa = 11.84 TKK36 pKa = 9.17 RR37 pKa = 11.84 TPGTTVTTTTTTRR50 pKa = 11.84 TTRR53 pKa = 11.84 TAGGHH58 pKa = 6.24 HH59 pKa = 6.1 SHH61 pKa = 7.22 AAPVHH66 pKa = 4.45 HH67 pKa = 7.29 HH68 pKa = 6.34 KK69 pKa = 10.63 RR70 pKa = 11.84 HH71 pKa = 4.81 ATMGDD76 pKa = 3.45 KK77 pKa = 10.91 VSGMMMKK84 pKa = 10.69 LKK86 pKa = 10.71 GSLTHH91 pKa = 7.24 KK92 pKa = 10.05 PGLKK96 pKa = 10.08 AAGTRR101 pKa = 11.84 RR102 pKa = 11.84 MHH104 pKa = 5.92 GTDD107 pKa = 2.72 GRR109 pKa = 11.84 NARR112 pKa = 11.84 RR113 pKa = 11.84 VYY115 pKa = 10.89
Molecular weight: 12.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.382
IPC2_protein 10.921
IPC_protein 12.384
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.266
Grimsley 12.588
Solomon 13.042
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 11.974
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.068
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9079
0
9079
5419111
9
10241
596.9
65.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.957 ± 0.018
1.151 ± 0.008
5.728 ± 0.017
6.271 ± 0.023
3.632 ± 0.015
6.822 ± 0.02
2.448 ± 0.009
4.716 ± 0.016
5.051 ± 0.021
8.454 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.255 ± 0.009
3.751 ± 0.013
6.262 ± 0.03
4.179 ± 0.02
5.978 ± 0.019
8.121 ± 0.028
6.073 ± 0.016
5.938 ± 0.016
1.434 ± 0.008
2.778 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here