Stemphylium lycopersici

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae; Stemphylium

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9079 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A364N1R0|A0A364N1R0_9PLEO 3'(2') 5'-bisphosphate nucleotidase OS=Stemphylium lycopersici OX=183478 GN=DDE83_005700 PE=3 SV=1
MM1 pKa = 7.56GFLSEE6 pKa = 5.51LISDD10 pKa = 4.66FKK12 pKa = 11.33GSSSSGQGDD21 pKa = 3.45QQQYY25 pKa = 7.46QQQQSGYY32 pKa = 8.11SQYY35 pKa = 10.82PPQQHH40 pKa = 6.18SPQPPPVSPPWYY52 pKa = 10.12AEE54 pKa = 3.24WDD56 pKa = 3.64SRR58 pKa = 11.84DD59 pKa = 3.57NRR61 pKa = 11.84WFFVNQHH68 pKa = 4.82TGEE71 pKa = 3.89RR72 pKa = 11.84TYY74 pKa = 11.12NYY76 pKa = 8.05PGPGYY81 pKa = 10.39AQQQGDD87 pKa = 4.14YY88 pKa = 10.36GAPPPQGGYY97 pKa = 10.98GYY99 pKa = 10.91GQGGYY104 pKa = 9.44SNQGAYY110 pKa = 8.34EE111 pKa = 3.94QSAYY115 pKa = 10.9DD116 pKa = 4.09PTRR119 pKa = 11.84SAQPQEE125 pKa = 4.31EE126 pKa = 4.35KK127 pKa = 10.89SGGNGWKK134 pKa = 10.01YY135 pKa = 10.66AAAGAAGLAGGALLMHH151 pKa = 7.09EE152 pKa = 4.55SDD154 pKa = 4.96KK155 pKa = 11.49IEE157 pKa = 5.32DD158 pKa = 3.72GWDD161 pKa = 3.28SAKK164 pKa = 10.81QNISNGVDD172 pKa = 3.01EE173 pKa = 5.35FGNDD177 pKa = 2.95VSNFPEE183 pKa = 4.46NAAGWTGEE191 pKa = 3.98QVGRR195 pKa = 11.84AEE197 pKa = 4.13NFGDD201 pKa = 4.81DD202 pKa = 5.56VEE204 pKa = 4.72QKK206 pKa = 10.13WDD208 pKa = 3.9DD209 pKa = 3.58GVQDD213 pKa = 4.27VEE215 pKa = 5.22DD216 pKa = 4.61FPEE219 pKa = 4.75NAAEE223 pKa = 4.01WTGEE227 pKa = 4.0KK228 pKa = 10.02VQEE231 pKa = 4.45VEE233 pKa = 6.29DD234 pKa = 3.98IPQDD238 pKa = 4.93IEE240 pKa = 4.54NKK242 pKa = 7.83WDD244 pKa = 3.4NMTEE248 pKa = 4.27GIEE251 pKa = 4.44QFGDD255 pKa = 3.8DD256 pKa = 4.41MGDD259 pKa = 3.29AYY261 pKa = 11.01DD262 pKa = 4.0EE263 pKa = 4.69GRR265 pKa = 11.84DD266 pKa = 3.59EE267 pKa = 5.28QRR269 pKa = 11.84YY270 pKa = 8.89EE271 pKa = 4.05DD272 pKa = 5.21DD273 pKa = 3.71YY274 pKa = 12.24

Molecular weight: 30.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A364N615|A0A364N615_9PLEO Replication factor A protein 3 OS=Stemphylium lycopersici OX=183478 GN=DDE83_003943 PE=3 SV=1
MM1 pKa = 7.54PLARR5 pKa = 11.84RR6 pKa = 11.84HH7 pKa = 5.57APRR10 pKa = 11.84TARR13 pKa = 11.84ATRR16 pKa = 11.84TTARR20 pKa = 11.84PSLKK24 pKa = 8.96TRR26 pKa = 11.84LMGGRR31 pKa = 11.84KK32 pKa = 7.3TRR34 pKa = 11.84TKK36 pKa = 9.17RR37 pKa = 11.84TPGTTVTTTTTTRR50 pKa = 11.84TTRR53 pKa = 11.84TAGGHH58 pKa = 6.24HH59 pKa = 6.1SHH61 pKa = 7.22AAPVHH66 pKa = 4.45HH67 pKa = 7.29HH68 pKa = 6.34KK69 pKa = 10.63RR70 pKa = 11.84HH71 pKa = 4.81ATMGDD76 pKa = 3.45KK77 pKa = 10.91VSGMMMKK84 pKa = 10.69LKK86 pKa = 10.71GSLTHH91 pKa = 7.24KK92 pKa = 10.05PGLKK96 pKa = 10.08AAGTRR101 pKa = 11.84RR102 pKa = 11.84MHH104 pKa = 5.92GTDD107 pKa = 2.72GRR109 pKa = 11.84NARR112 pKa = 11.84RR113 pKa = 11.84VYY115 pKa = 10.89

Molecular weight: 12.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9079

0

9079

5419111

9

10241

596.9

65.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.957 ± 0.018

1.151 ± 0.008

5.728 ± 0.017

6.271 ± 0.023

3.632 ± 0.015

6.822 ± 0.02

2.448 ± 0.009

4.716 ± 0.016

5.051 ± 0.021

8.454 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.255 ± 0.009

3.751 ± 0.013

6.262 ± 0.03

4.179 ± 0.02

5.978 ± 0.019

8.121 ± 0.028

6.073 ± 0.016

5.938 ± 0.016

1.434 ± 0.008

2.778 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski