Paenibacillus sp. YN15

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5879 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A329LCR8|A0A329LCR8_9BACL Uncharacterized protein OS=Paenibacillus sp. YN15 OX=1742774 GN=DQG13_04040 PE=4 SV=1
MM1 pKa = 7.58TEE3 pKa = 4.3HH4 pKa = 6.74NHH6 pKa = 7.24DD7 pKa = 4.36CGCGHH12 pKa = 7.17DD13 pKa = 4.41HH14 pKa = 7.41DD15 pKa = 5.58HH16 pKa = 6.8DD17 pKa = 4.33HH18 pKa = 6.69EE19 pKa = 4.63EE20 pKa = 4.18EE21 pKa = 4.23VFLIADD27 pKa = 3.59EE28 pKa = 4.63EE29 pKa = 4.71GVEE32 pKa = 4.14RR33 pKa = 11.84EE34 pKa = 3.91MVMVYY39 pKa = 10.39SFEE42 pKa = 4.35CDD44 pKa = 3.08EE45 pKa = 4.21NVYY48 pKa = 10.96AVLLDD53 pKa = 3.9RR54 pKa = 11.84NNPEE58 pKa = 3.48SDD60 pKa = 3.25GVIFRR65 pKa = 11.84IEE67 pKa = 3.93QEE69 pKa = 4.18GEE71 pKa = 3.5DD72 pKa = 3.69AYY74 pKa = 11.44LVNIEE79 pKa = 5.5NDD81 pKa = 3.72EE82 pKa = 4.07EE83 pKa = 4.31WQKK86 pKa = 10.97VVAAYY91 pKa = 10.5DD92 pKa = 3.79EE93 pKa = 4.64IVANEE98 pKa = 3.93QQQ100 pKa = 3.06

Molecular weight: 11.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A329K8W9|A0A329K8W9_9BACL Orotate phosphoribosyltransferase OS=Paenibacillus sp. YN15 OX=1742774 GN=pyrE PE=3 SV=1
MM1 pKa = 7.41KK2 pKa = 10.1PSFNPNVSKK11 pKa = 10.69RR12 pKa = 11.84KK13 pKa = 9.0KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.98VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.09VLTAA44 pKa = 4.32

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5879

0

5879

2125208

26

3393

361.5

40.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.383 ± 0.048

0.819 ± 0.01

4.976 ± 0.024

6.779 ± 0.034

3.998 ± 0.022

7.992 ± 0.037

1.948 ± 0.016

5.83 ± 0.03

4.907 ± 0.029

10.2 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.725 ± 0.017

3.567 ± 0.021

4.352 ± 0.024

3.693 ± 0.019

5.511 ± 0.03

6.072 ± 0.025

5.261 ± 0.031

6.974 ± 0.021

1.386 ± 0.013

3.627 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski