Murine feces-associated gemycircularvirus 1
Average proteome isoelectric point is 7.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S0SZ49|A0A2S0SZ49_9VIRU Cap OS=Murine feces-associated gemycircularvirus 1 OX=2171384 PE=4 SV=1
MM1 pKa = 7.39 NPGPGPWSSTMLYY14 pKa = 10.12 SGPSDD19 pKa = 3.42 PLEE22 pKa = 4.32 NLCHH26 pKa = 6.68 HH27 pKa = 7.09 SDD29 pKa = 3.72 SKK31 pKa = 11.62 HH32 pKa = 7.23 DD33 pKa = 4.04 MDD35 pKa = 6.14 SLPIPNADD43 pKa = 3.32 DD44 pKa = 4.04 SVLSEE49 pKa = 4.31 YY50 pKa = 10.79 PNITRR55 pKa = 11.84 LLVQISSSAAKK66 pKa = 8.25 RR67 pKa = 11.84 TAMVEE72 pKa = 4.13 RR73 pKa = 11.84 ISMFLQILSEE83 pKa = 4.14 SSEE86 pKa = 4.45 LEE88 pKa = 4.05 TVANSMLRR96 pKa = 11.84 TSIQISFLHH105 pKa = 6.98 SEE107 pKa = 3.88 ILLAVGTMQRR117 pKa = 11.84 RR118 pKa = 11.84 MATFVAGNYY127 pKa = 8.23 EE128 pKa = 4.12 DD129 pKa = 4.56 QNLLEE134 pKa = 4.3 VLKK137 pKa = 10.68 EE138 pKa = 3.81 ADD140 pKa = 3.32 KK141 pKa = 11.59 TMDD144 pKa = 3.56 GTDD147 pKa = 4.3 SEE149 pKa = 4.52 LQSLGTSFSEE159 pKa = 3.99 LRR161 pKa = 11.84 ASISLVTWSNPSTASVHH178 pKa = 5.04 TQTGSLEE185 pKa = 4.15 WIDD188 pKa = 5.52 RR189 pKa = 11.84 LMCTIQNSRR198 pKa = 11.84 SVIHH202 pKa = 6.05 NWMAFGNGLGSFLPVANMVCIAPCTLGCSQSHH234 pKa = 5.85 SPPLRR239 pKa = 11.84 GGDD242 pKa = 3.48 FHH244 pKa = 7.77 DD245 pKa = 4.48 AEE247 pKa = 4.61 ARR249 pKa = 11.84 RR250 pKa = 11.84 SPSVAGLAWRR260 pKa = 11.84 RR261 pKa = 11.84 QEE263 pKa = 3.64 EE264 pKa = 4.23 WAFTNWII271 pKa = 3.85
Molecular weight: 29.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.005
IPC2_protein 5.029
IPC_protein 4.965
Toseland 4.952
ProMoST 5.13
Dawson 4.991
Bjellqvist 5.105
Wikipedia 4.863
Rodwell 4.914
Grimsley 4.889
Solomon 4.991
Lehninger 4.952
Nozaki 5.118
DTASelect 5.27
Thurlkill 4.978
EMBOSS 4.927
Sillero 5.194
Patrickios 3.961
IPC_peptide 5.003
IPC2_peptide 5.194
IPC2.peptide.svr19 5.135
Protein with the highest isoelectric point:
>tr|A0A2S0SZ49|A0A2S0SZ49_9VIRU Cap OS=Murine feces-associated gemycircularvirus 1 OX=2171384 PE=4 SV=1
MM1 pKa = 7.65 AYY3 pKa = 10.07 SRR5 pKa = 11.84 RR6 pKa = 11.84 SRR8 pKa = 11.84 FRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 YY14 pKa = 7.44 PARR17 pKa = 11.84 RR18 pKa = 11.84 YY19 pKa = 9.43 ARR21 pKa = 11.84 RR22 pKa = 11.84 SFRR25 pKa = 11.84 SPRR28 pKa = 11.84 FARR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 TTRR38 pKa = 11.84 MSTRR42 pKa = 11.84 RR43 pKa = 11.84 VRR45 pKa = 11.84 NLAARR50 pKa = 11.84 KK51 pKa = 9.7 CRR53 pKa = 11.84 DD54 pKa = 3.32 NMLSTPRR61 pKa = 11.84 NDD63 pKa = 3.49 AGEE66 pKa = 4.17 VQPAQPYY73 pKa = 10.69 LMTGGTSYY81 pKa = 11.26 RR82 pKa = 11.84 FLFSPSFRR90 pKa = 11.84 SVGSIIGPEE99 pKa = 3.86 EE100 pKa = 3.93 RR101 pKa = 11.84 QISASAAEE109 pKa = 4.07 RR110 pKa = 11.84 WKK112 pKa = 10.58 SKK114 pKa = 10.7 CYY116 pKa = 8.6 MRR118 pKa = 11.84 GYY120 pKa = 10.48 KK121 pKa = 8.78 EE122 pKa = 4.22 TVEE125 pKa = 5.38 LEE127 pKa = 4.52 TNDD130 pKa = 3.22 SSSWLWRR137 pKa = 11.84 RR138 pKa = 11.84 IVFTTLGWGAPLADD152 pKa = 3.95 YY153 pKa = 10.56 IAANVTQRR161 pKa = 11.84 GYY163 pKa = 11.34 GRR165 pKa = 11.84 LMYY168 pKa = 10.98 NLDD171 pKa = 3.78 NGQHH175 pKa = 6.5 DD176 pKa = 4.62 VLLGDD181 pKa = 4.08 AYY183 pKa = 11.48 SRR185 pKa = 11.84 LFEE188 pKa = 4.18 GQRR191 pKa = 11.84 TVDD194 pKa = 3.06 WVNPLTAKK202 pKa = 10.0 VDD204 pKa = 3.4 SRR206 pKa = 11.84 RR207 pKa = 11.84 IRR209 pKa = 11.84 VISDD213 pKa = 3.19 TLRR216 pKa = 11.84 TIQSPNSEE224 pKa = 4.05 SGRR227 pKa = 11.84 YY228 pKa = 7.52 QVTKK232 pKa = 9.83 RR233 pKa = 11.84 WYY235 pKa = 8.53 PVNKK239 pKa = 10.18 SIIYY243 pKa = 10.47 DD244 pKa = 3.64 DD245 pKa = 4.93 DD246 pKa = 4.66 EE247 pKa = 6.27 NGTAHH252 pKa = 7.12 KK253 pKa = 10.33 FSVGWASGAAQANAGDD269 pKa = 4.38 LFVFDD274 pKa = 4.87 MFDD277 pKa = 3.19 AATDD281 pKa = 3.65 ATEE284 pKa = 4.23 DD285 pKa = 3.36 TTLAMGTSGTYY296 pKa = 8.98 YY297 pKa = 8.22 WHH299 pKa = 7.07 EE300 pKa = 4.0 GSGRR304 pKa = 3.49
Molecular weight: 34.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.31
IPC2_protein 9.78
IPC_protein 10.745
Toseland 10.35
ProMoST 10.365
Dawson 10.584
Bjellqvist 10.394
Wikipedia 10.877
Rodwell 10.599
Grimsley 10.687
Solomon 10.672
Lehninger 10.613
Nozaki 10.35
DTASelect 10.379
Thurlkill 10.438
EMBOSS 10.789
Sillero 10.511
Patrickios 9.984
IPC_peptide 10.672
IPC2_peptide 9.487
IPC2.peptide.svr19 8.457
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
575
271
304
287.5
32.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.0 ± 0.682
1.217 ± 0.433
5.391 ± 0.41
4.87 ± 0.713
3.478 ± 0.363
6.435 ± 0.62
1.913 ± 0.716
4.174 ± 0.684
2.087 ± 0.421
7.826 ± 1.727
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.478 ± 0.655
4.348 ± 0.31
4.348 ± 0.564
3.304 ± 0.266
9.913 ± 3.271
11.478 ± 1.753
6.957 ± 0.471
4.87 ± 0.05
2.609 ± 0.018
3.304 ± 1.514
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here