Arsenophonus endosymbiont of Bemisia tabaci Asia II 3
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1589 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q1BE90|A0A4Q1BE90_9GAMM XRE family transcriptional regulator OS=Arsenophonus endosymbiont of Bemisia tabaci Asia II 3 OX=1868623 GN=BBD39_07365 PE=4 SV=1
MM1 pKa = 7.49 FEE3 pKa = 4.36 WLVQDD8 pKa = 4.28 RR9 pKa = 11.84 FCFSLGLSEE18 pKa = 4.34 EE19 pKa = 4.44 DD20 pKa = 2.94 EE21 pKa = 4.47 AVFVEE26 pKa = 4.66 LFSVGKK32 pKa = 10.16 AADD35 pKa = 3.23 GRR37 pKa = 11.84 YY38 pKa = 9.51 AVLDD42 pKa = 3.47 IAEE45 pKa = 4.62 IGHH48 pKa = 6.84 DD49 pKa = 3.7 FSDD52 pKa = 3.68 AQGAGWVDD60 pKa = 3.63 CVLVLVFCVYY70 pKa = 10.72 LVDD73 pKa = 6.05 DD74 pKa = 4.66 LFEE77 pKa = 4.13
Molecular weight: 8.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.762
IPC2_protein 3.681
IPC_protein 3.617
Toseland 3.414
ProMoST 3.757
Dawson 3.617
Bjellqvist 3.846
Wikipedia 3.567
Rodwell 3.452
Grimsley 3.338
Solomon 3.579
Lehninger 3.541
Nozaki 3.745
DTASelect 3.935
Thurlkill 3.49
EMBOSS 3.579
Sillero 3.745
Patrickios 0.693
IPC_peptide 3.579
IPC2_peptide 3.706
IPC2.peptide.svr19 3.715
Protein with the highest isoelectric point:
>tr|A0A4Q1B3G5|A0A4Q1B3G5_9GAMM Uncharacterized protein OS=Arsenophonus endosymbiont of Bemisia tabaci Asia II 3 OX=1868623 GN=BBD39_03450 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 NRR14 pKa = 11.84 SHH16 pKa = 7.16 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.29 ARR39 pKa = 11.84 ARR41 pKa = 11.84 LTVSSKK47 pKa = 11.16
Molecular weight: 5.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1589
0
1589
407097
38
1482
256.2
28.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.052 ± 0.061
1.113 ± 0.022
5.178 ± 0.041
5.881 ± 0.062
3.922 ± 0.045
6.421 ± 0.056
2.266 ± 0.029
7.828 ± 0.072
5.955 ± 0.049
10.407 ± 0.074
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.55 ± 0.032
4.824 ± 0.059
3.9 ± 0.043
4.748 ± 0.055
5.042 ± 0.056
6.05 ± 0.05
5.3 ± 0.04
6.188 ± 0.054
1.205 ± 0.028
3.17 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here