Streptococcus phage Javan241
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6A649|A0A4D6A649_9CAUD Uncharacterized protein OS=Streptococcus phage Javan241 OX=2548065 GN=Javan241_0005 PE=4 SV=1
MM1 pKa = 6.43 TTVNEE6 pKa = 4.18 ALNDD10 pKa = 3.99 LSALVYY16 pKa = 10.75 SGTPVGNGEE25 pKa = 4.42 CYY27 pKa = 10.82 ALASYY32 pKa = 10.59 YY33 pKa = 10.57 EE34 pKa = 4.24 SLINPDD40 pKa = 3.04 GTVGLGAGVGYY51 pKa = 10.77 VSGAIGDD58 pKa = 4.43 TICAANIGTSYY69 pKa = 11.07 DD70 pKa = 2.85 WSANGWSVSSDD81 pKa = 4.1 DD82 pKa = 4.21 EE83 pKa = 4.31 LQPGQILTIQANAYY97 pKa = 9.18 NEE99 pKa = 4.18 YY100 pKa = 8.53 GHH102 pKa = 6.13 VVVVEE107 pKa = 4.24 AVDD110 pKa = 4.19 GDD112 pKa = 3.85 QLTVIEE118 pKa = 4.31 QNYY121 pKa = 10.0 AGARR125 pKa = 11.84 YY126 pKa = 8.23 PVRR129 pKa = 11.84 NYY131 pKa = 10.66 YY132 pKa = 10.02 SASEE136 pKa = 4.1 YY137 pKa = 9.63 QQTVAHH143 pKa = 6.67 YY144 pKa = 7.2 ITPPGAEE151 pKa = 4.2 TTEE154 pKa = 4.4 SASDD158 pKa = 3.66 TNTSTTSTEE167 pKa = 3.86 PNSYY171 pKa = 10.62 KK172 pKa = 10.47 EE173 pKa = 4.45 DD174 pKa = 3.17 GTMTVTVDD182 pKa = 4.2 AINVRR187 pKa = 11.84 RR188 pKa = 11.84 SPDD191 pKa = 3.0 TSGEE195 pKa = 4.05 VVAQYY200 pKa = 10.62 TKK202 pKa = 10.75 GQSFKK207 pKa = 10.33 YY208 pKa = 8.72 DD209 pKa = 3.48 TVIIDD214 pKa = 3.42 YY215 pKa = 11.16 NGFVWVSYY223 pKa = 10.79 VGASGNRR230 pKa = 11.84 NYY232 pKa = 10.56 VATGPTQNGKK242 pKa = 9.79 RR243 pKa = 11.84 FGDD246 pKa = 2.84 AWGTFKK252 pKa = 11.14
Molecular weight: 26.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.025
IPC2_protein 4.101
IPC_protein 4.05
Toseland 3.859
ProMoST 4.19
Dawson 4.024
Bjellqvist 4.177
Wikipedia 3.935
Rodwell 3.884
Grimsley 3.77
Solomon 4.024
Lehninger 3.973
Nozaki 4.139
DTASelect 4.342
Thurlkill 3.897
EMBOSS 3.948
Sillero 4.164
Patrickios 0.146
IPC_peptide 4.024
IPC2_peptide 4.151
IPC2.peptide.svr19 4.074
Protein with the highest isoelectric point:
>tr|A0A4D6A7I5|A0A4D6A7I5_9CAUD Uncharacterized protein OS=Streptococcus phage Javan241 OX=2548065 GN=Javan241_0046 PE=4 SV=1
MM1 pKa = 7.66 ADD3 pKa = 3.04 VRR5 pKa = 11.84 VVVDD9 pKa = 3.89 LGGVDD14 pKa = 3.77 RR15 pKa = 11.84 KK16 pKa = 10.56 LSPEE20 pKa = 3.65 AEE22 pKa = 4.22 KK23 pKa = 10.68 RR24 pKa = 11.84 GKK26 pKa = 10.21 LAMASQGMMIMEE38 pKa = 4.53 SYY40 pKa = 10.53 IPLRR44 pKa = 11.84 GGPLRR49 pKa = 11.84 ASGRR53 pKa = 11.84 IEE55 pKa = 3.98 ANGDD59 pKa = 2.59 ISYY62 pKa = 8.34 NTVYY66 pKa = 10.91 ARR68 pKa = 11.84 AHH70 pKa = 6.28 FYY72 pKa = 9.96 GANGIVVFRR81 pKa = 11.84 RR82 pKa = 11.84 YY83 pKa = 7.67 RR84 pKa = 11.84 TPGTGKK90 pKa = 10.07 RR91 pKa = 11.84 WDD93 pKa = 3.71 RR94 pKa = 11.84 ALKK97 pKa = 10.94 ANVEE101 pKa = 4.14 KK102 pKa = 10.75 LKK104 pKa = 10.68 QVAVRR109 pKa = 11.84 AMGLRR114 pKa = 3.59
Molecular weight: 12.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.326
IPC2_protein 9.867
IPC_protein 10.73
Toseland 10.745
ProMoST 10.716
Dawson 10.847
Bjellqvist 10.584
Wikipedia 11.082
Rodwell 11.052
Grimsley 10.906
Solomon 10.965
Lehninger 10.935
Nozaki 10.701
DTASelect 10.584
Thurlkill 10.745
EMBOSS 11.14
Sillero 10.774
Patrickios 10.774
IPC_peptide 10.979
IPC2_peptide 9.428
IPC2.peptide.svr19 8.583
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
48
0
48
10945
48
1290
228.0
25.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.775 ± 1.018
0.457 ± 0.079
6.725 ± 0.449
6.971 ± 0.638
4.111 ± 0.224
6.633 ± 0.579
1.151 ± 0.155
6.88 ± 0.285
7.784 ± 0.433
8.287 ± 0.362
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.503 ± 0.243
5.793 ± 0.388
2.805 ± 0.231
4.358 ± 0.211
4.157 ± 0.376
6.578 ± 0.306
5.747 ± 0.28
6.423 ± 0.355
0.978 ± 0.126
3.883 ± 0.341
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here