Yerba mate chlorosis-associated virus
Average proteome isoelectric point is 7.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W5S0M4|A0A1W5S0M4_9RHAB Large structural protein OS=Yerba mate chlorosis-associated virus OX=2487100 PE=4 SV=1
MM1 pKa = 7.67 SNGFEE6 pKa = 4.14 FKK8 pKa = 10.61 KK9 pKa = 10.54 IEE11 pKa = 4.17 GKK13 pKa = 10.36 LDD15 pKa = 3.52 YY16 pKa = 11.19 SGVQHH21 pKa = 6.39 MSSALDD27 pKa = 4.06 DD28 pKa = 5.12 DD29 pKa = 5.74 NMFADD34 pKa = 4.35 KK35 pKa = 10.85 NVSLEE40 pKa = 4.07 NKK42 pKa = 10.29 EE43 pKa = 4.42 EE44 pKa = 4.21 GTKK47 pKa = 8.94 TAEE50 pKa = 3.82 IVVTQNIPAASKK62 pKa = 10.26 EE63 pKa = 4.26 KK64 pKa = 10.73 NSPFLKK70 pKa = 10.51 ALSKK74 pKa = 9.97 KK75 pKa = 9.17 TEE77 pKa = 4.47 DD78 pKa = 3.42 NTGKK82 pKa = 10.28 SGEE85 pKa = 4.21 VEE87 pKa = 3.94 EE88 pKa = 5.1 SKK90 pKa = 9.87 TPKK93 pKa = 9.89 DD94 pKa = 3.01 TDD96 pKa = 3.4 KK97 pKa = 11.61 AKK99 pKa = 8.69 EE100 pKa = 4.25 TKK102 pKa = 9.81 SVKK105 pKa = 9.95 SLKK108 pKa = 10.4 EE109 pKa = 3.75 NLKK112 pKa = 10.73 SRR114 pKa = 11.84 LEE116 pKa = 4.0 DD117 pKa = 3.23 HH118 pKa = 7.35 LGRR121 pKa = 11.84 SSNNSKK127 pKa = 10.9 EE128 pKa = 4.04 EE129 pKa = 4.02 NKK131 pKa = 10.42 DD132 pKa = 3.26 SKK134 pKa = 11.32 AVEE137 pKa = 4.08 KK138 pKa = 10.12 TKK140 pKa = 11.19 KK141 pKa = 10.29 NLSKK145 pKa = 10.88 DD146 pKa = 3.43 LNKK149 pKa = 9.96 VLEE152 pKa = 4.35 EE153 pKa = 4.29 DD154 pKa = 4.2 EE155 pKa = 4.87 EE156 pKa = 5.36 IEE158 pKa = 5.03 DD159 pKa = 3.67 EE160 pKa = 4.46 SEE162 pKa = 4.0 EE163 pKa = 4.1 LFSPEE168 pKa = 4.77 EE169 pKa = 3.81 IRR171 pKa = 11.84 DD172 pKa = 4.06 CLDD175 pKa = 3.51 LFCNKK180 pKa = 10.11 LGVDD184 pKa = 5.23 GEE186 pKa = 4.82 SAWYY190 pKa = 9.02 NHH192 pKa = 5.99 FVASLNINGAVFSSEE207 pKa = 3.66 IEE209 pKa = 4.15 SWVSAIKK216 pKa = 10.42 HH217 pKa = 4.7 EE218 pKa = 4.37 RR219 pKa = 11.84 ATASHH224 pKa = 6.63 RR225 pKa = 11.84 AIKK228 pKa = 10.3 EE229 pKa = 3.42 ILTNLNTQVVRR240 pKa = 11.84 LTAQVKK246 pKa = 10.11 ALSDD250 pKa = 3.93 TNGSLAKK257 pKa = 10.44 QNEE260 pKa = 4.55 SLLNMVDD267 pKa = 3.84 SMRR270 pKa = 11.84 KK271 pKa = 8.04 NQEE274 pKa = 3.13 EE275 pKa = 4.29 WLNTQLEE282 pKa = 4.39 LRR284 pKa = 11.84 QGAIRR289 pKa = 11.84 GGYY292 pKa = 8.3 QKK294 pKa = 10.93 EE295 pKa = 4.14 KK296 pKa = 10.99 GKK298 pKa = 8.89 LTEE301 pKa = 4.17 EE302 pKa = 4.24 MSDD305 pKa = 3.56 EE306 pKa = 4.23 NASSGSYY313 pKa = 10.72 YY314 pKa = 10.62 LDD316 pKa = 3.4 EE317 pKa = 4.99 TDD319 pKa = 5.95 DD320 pKa = 3.82 EE321 pKa = 4.61 TSEE324 pKa = 4.51 LLVSHH329 pKa = 7.39 FLTLLKK335 pKa = 10.47 INSTQIQDD343 pKa = 3.3 PRR345 pKa = 11.84 VRR347 pKa = 11.84 MAVRR351 pKa = 11.84 SLFPMKK357 pKa = 10.19 ILVAACEE364 pKa = 4.0 HH365 pKa = 7.29 GIRR368 pKa = 11.84 EE369 pKa = 3.98 ADD371 pKa = 3.41 KK372 pKa = 10.94 PKK374 pKa = 10.73 ALEE377 pKa = 4.32 KK378 pKa = 10.49 LKK380 pKa = 10.81 EE381 pKa = 4.14 WILNNN386 pKa = 3.72
Molecular weight: 43.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.185
IPC2_protein 5.156
IPC_protein 5.092
Toseland 5.016
ProMoST 5.194
Dawson 5.054
Bjellqvist 5.181
Wikipedia 4.889
Rodwell 4.978
Grimsley 4.94
Solomon 5.054
Lehninger 5.003
Nozaki 5.169
DTASelect 5.27
Thurlkill 5.003
EMBOSS 4.927
Sillero 5.245
Patrickios 4.533
IPC_peptide 5.054
IPC2_peptide 5.245
IPC2.peptide.svr19 5.223
Protein with the highest isoelectric point:
>tr|A0A1W5RYH2|A0A1W5RYH2_9RHAB Phosphoprotein OS=Yerba mate chlorosis-associated virus OX=2487100 PE=4 SV=1
MM1 pKa = 7.24 FFIIKK6 pKa = 9.58 PLIKK10 pKa = 10.52 LLVYY14 pKa = 8.9 LLCLFFLLSVLVWGCIMMCDD34 pKa = 5.12 LYY36 pKa = 11.18 EE37 pKa = 4.06 CHH39 pKa = 7.0 NNLKK43 pKa = 10.84 LNIISMKK50 pKa = 10.6 KK51 pKa = 10.57 FIDD54 pKa = 3.73 CVKK57 pKa = 10.45 KK58 pKa = 10.32 KK59 pKa = 10.07 RR60 pKa = 11.84 QNPFCPLL67 pKa = 3.2
Molecular weight: 8.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.461
IPC2_protein 8.273
IPC_protein 8.2
Toseland 8.141
ProMoST 8.697
Dawson 8.916
Bjellqvist 9.194
Wikipedia 8.858
Rodwell 8.99
Grimsley 7.819
Solomon 9.136
Lehninger 9.136
Nozaki 9.531
DTASelect 8.916
Thurlkill 9.033
EMBOSS 9.136
Sillero 9.326
Patrickios 4.202
IPC_peptide 9.121
IPC2_peptide 8.317
IPC2.peptide.svr19 8.136
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
4015
67
2134
573.6
65.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.359 ± 0.752
1.893 ± 0.451
5.355 ± 0.496
6.426 ± 0.972
4.633 ± 0.452
5.056 ± 0.256
2.391 ± 0.297
7.547 ± 0.72
8.02 ± 0.818
10.66 ± 0.439
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.64 ± 0.291
5.305 ± 0.448
4.209 ± 0.472
3.362 ± 0.426
3.985 ± 0.596
8.344 ± 0.741
5.28 ± 0.766
5.006 ± 0.234
1.694 ± 0.211
3.836 ± 0.569
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here