Escherichia phage 4MG

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Vequintavirinae; Seunavirus; Escherichia virus 4MG

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 271 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5KSM2|V5KSM2_9CAUD Hyphothetical protein OS=Escherichia phage 4MG OX=1391428 GN=4MG_097 PE=4 SV=1
MM1 pKa = 7.37GLSMDD6 pKa = 4.91FPVEE10 pKa = 3.8MSDD13 pKa = 3.79YY14 pKa = 10.32LPHH17 pKa = 7.59IVGDD21 pKa = 3.76LRR23 pKa = 11.84NGGFTVDD30 pKa = 3.73IIGYY34 pKa = 8.19QPTMQGTSINFFVTWW49 pKa = 3.46

Molecular weight: 5.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5KSH0|V5KSH0_9CAUD Cell wall hydrolase SleB OS=Escherichia phage 4MG OX=1391428 GN=4MG_270 PE=4 SV=1
MM1 pKa = 7.49NLLTLLRR8 pKa = 11.84TNKK11 pKa = 8.58YY12 pKa = 5.49TQKK15 pKa = 10.96QMGFLLGIPSWKK27 pKa = 9.77VSKK30 pKa = 10.66LVLKK34 pKa = 10.75LEE36 pKa = 4.15SRR38 pKa = 11.84GYY40 pKa = 8.47IQVNRR45 pKa = 11.84RR46 pKa = 11.84YY47 pKa = 9.47KK48 pKa = 10.7LSGPVSRR55 pKa = 11.84PFLIYY60 pKa = 10.34DD61 pKa = 3.25QNLYY65 pKa = 10.68KK66 pKa = 10.01VTLYY70 pKa = 10.48RR71 pKa = 11.84SKK73 pKa = 10.38EE74 pKa = 3.97CPEE77 pKa = 4.01IKK79 pKa = 9.31VTPTKK84 pKa = 9.76EE85 pKa = 3.83QSRR88 pKa = 11.84SRR90 pKa = 11.84DD91 pKa = 3.39SVGRR95 pKa = 11.84GLRR98 pKa = 11.84NFAKK102 pKa = 10.49HH103 pKa = 6.29LVTGCLTSIRR113 pKa = 11.84RR114 pKa = 11.84PEE116 pKa = 3.61RR117 pKa = 4.35

Molecular weight: 13.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

271

0

271

45138

29

929

166.6

18.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.331 ± 0.189

1.218 ± 0.073

6.6 ± 0.121

6.655 ± 0.19

4.265 ± 0.093

7.236 ± 0.262

1.788 ± 0.088

5.771 ± 0.122

6.764 ± 0.221

7.809 ± 0.145

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.96 ± 0.112

4.688 ± 0.131

3.771 ± 0.148

3.418 ± 0.114

4.92 ± 0.155

5.784 ± 0.13

6.046 ± 0.213

7.209 ± 0.159

1.788 ± 0.08

3.979 ± 0.128
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski