Streptococcus phage phiARI0923

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A141DZB4|A0A141DZB4_9CAUD Uncharacterized protein OS=Streptococcus phage phiARI0923 OX=1701842 GN=phiARI0923_15 PE=4 SV=1
MM1 pKa = 7.73TILNQIKK8 pKa = 10.15EE9 pKa = 4.27MVEE12 pKa = 3.65VDD14 pKa = 3.55VEE16 pKa = 4.23EE17 pKa = 5.21EE18 pKa = 3.87IFDD21 pKa = 4.09TQFLSYY27 pKa = 9.83INSGISYY34 pKa = 7.81LTRR37 pKa = 11.84NNIPINRR44 pKa = 11.84IDD46 pKa = 4.12KK47 pKa = 10.41EE48 pKa = 4.44SEE50 pKa = 3.7LTEE53 pKa = 3.96WNEE56 pKa = 3.66IEE58 pKa = 4.93EE59 pKa = 4.49DD60 pKa = 3.81DD61 pKa = 5.63KK62 pKa = 11.62EE63 pKa = 4.58TILDD67 pKa = 3.89WLHH70 pKa = 5.96LRR72 pKa = 11.84CVQRR76 pKa = 11.84FDD78 pKa = 3.62KK79 pKa = 11.23SLMTGNSTTRR89 pKa = 11.84SWIDD93 pKa = 3.33EE94 pKa = 4.18EE95 pKa = 4.49LTNILYY101 pKa = 9.15QLKK104 pKa = 10.35AIYY107 pKa = 9.57GVKK110 pKa = 10.05SS111 pKa = 3.24

Molecular weight: 13.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A141DZC0|A0A141DZC0_9CAUD Uncharacterized protein OS=Streptococcus phage phiARI0923 OX=1701842 GN=phiARI0923_21 PE=4 SV=1
MM1 pKa = 7.45NIVEE5 pKa = 4.55PLRR8 pKa = 11.84DD9 pKa = 3.59KK10 pKa = 11.6DD11 pKa = 5.25DD12 pKa = 3.5IQAMKK17 pKa = 10.51DD18 pKa = 3.7YY19 pKa = 10.79LSSWNEE25 pKa = 3.44KK26 pKa = 10.62YY27 pKa = 11.34YY28 pKa = 10.51MLFLLGINTGFRR40 pKa = 11.84VGDD43 pKa = 3.53ILKK46 pKa = 10.74LKK48 pKa = 10.58VKK50 pKa = 10.21DD51 pKa = 3.57VQGWHH56 pKa = 5.56IKK58 pKa = 9.73VRR60 pKa = 11.84EE61 pKa = 4.0QKK63 pKa = 8.08TGKK66 pKa = 9.91YY67 pKa = 10.06KK68 pKa = 10.27SIKK71 pKa = 6.49MTRR74 pKa = 11.84PLKK77 pKa = 10.13NEE79 pKa = 3.11LRR81 pKa = 11.84EE82 pKa = 3.98FVKK85 pKa = 10.81DD86 pKa = 3.94KK87 pKa = 10.69EE88 pKa = 4.01LHH90 pKa = 6.23EE91 pKa = 4.53YY92 pKa = 10.65LFQSRR97 pKa = 11.84VGKK100 pKa = 10.34NKK102 pKa = 9.41ALSYY106 pKa = 9.36KK107 pKa = 8.5TVYY110 pKa = 9.67WFLKK114 pKa = 10.04RR115 pKa = 11.84AAEE118 pKa = 4.39DD119 pKa = 3.61LGIDD123 pKa = 3.45NVGTHH128 pKa = 5.72TMRR131 pKa = 11.84KK132 pKa = 6.66TFGYY136 pKa = 9.98HH137 pKa = 5.21YY138 pKa = 9.53YY139 pKa = 10.76KK140 pKa = 10.53KK141 pKa = 10.06YY142 pKa = 11.08KK143 pKa = 10.39NVADD147 pKa = 4.17LMSLFNHH154 pKa = 6.53SSPAVTLIYY163 pKa = 10.26ICVRR167 pKa = 11.84QDD169 pKa = 3.4EE170 pKa = 5.43LDD172 pKa = 3.36TKK174 pKa = 10.49MSNFSLL180 pKa = 4.04

Molecular weight: 21.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

35

0

35

9574

67

2078

273.5

31.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.612 ± 0.518

0.554 ± 0.102

5.787 ± 0.391

7.792 ± 0.341

4.115 ± 0.24

6.204 ± 0.37

1.504 ± 0.195

7.092 ± 0.249

8.231 ± 0.294

8.523 ± 0.448

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.267 ± 0.286

6.121 ± 0.287

2.549 ± 0.199

4.063 ± 0.313

4.502 ± 0.31

6.591 ± 0.536

6.539 ± 0.389

5.881 ± 0.221

1.421 ± 0.166

3.656 ± 0.433
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski