Bicyclus anynana (Squinting bush brown butterfly)
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 19396 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6J1N4P6|A0A6J1N4P6_BICAN DNA mismatch repair protein Msh2 OS=Bicyclus anynana OX=110368 GN=LOC112045785 PE=3 SV=1
MM1 pKa = 7.25 SVSEE5 pKa = 4.36 GEE7 pKa = 4.34 GRR9 pKa = 11.84 AVWRR13 pKa = 11.84 LGEE16 pKa = 4.15 QGQHH20 pKa = 4.71 TLVVAARR27 pKa = 11.84 EE28 pKa = 3.99 ALAAPSALALPPPEE42 pKa = 4.56 PAPGLILPDD51 pKa = 4.24 GSINWGCPCLGGMATGPCGTQFRR74 pKa = 11.84 DD75 pKa = 3.29 AFSCFHH81 pKa = 6.3 YY82 pKa = 10.92 SEE84 pKa = 5.43 AEE86 pKa = 4.03 PKK88 pKa = 10.92 GSDD91 pKa = 3.58 CYY93 pKa = 11.65 DD94 pKa = 3.42 KK95 pKa = 10.99 FSVMQEE101 pKa = 3.8 CMAQYY106 pKa = 9.05 PQLYY110 pKa = 10.49 AKK112 pKa = 10.69 DD113 pKa = 4.5 DD114 pKa = 4.64 DD115 pKa = 4.91 DD116 pKa = 7.2 DD117 pKa = 4.2 EE118 pKa = 5.47 LADD121 pKa = 3.93 EE122 pKa = 5.5 LGAALQGAADD132 pKa = 4.29 ASDD135 pKa = 5.0 APDD138 pKa = 4.65 APDD141 pKa = 5.17 APDD144 pKa = 4.03 APPPGRR150 pKa = 4.02
Molecular weight: 15.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.923
IPC_protein 3.897
Toseland 3.681
ProMoST 4.062
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.821
Rodwell 3.719
Grimsley 3.592
Solomon 3.872
Lehninger 3.834
Nozaki 3.999
DTASelect 4.228
Thurlkill 3.745
EMBOSS 3.834
Sillero 4.012
Patrickios 1.036
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.893
Protein with the highest isoelectric point:
>tr|A0A6J1P5H1|A0A6J1P5H1_BICAN zinc metalloproteinase nas-4-like OS=Bicyclus anynana OX=110368 GN=LOC112056827 PE=4 SV=1
MM1 pKa = 7.44 SAHH4 pKa = 6.1 KK5 pKa = 9.54 TFIIKK10 pKa = 10.27 RR11 pKa = 11.84 KK12 pKa = 8.77 LARR15 pKa = 11.84 KK16 pKa = 9.26 LKK18 pKa = 8.96 QNRR21 pKa = 11.84 PIPQWVRR28 pKa = 11.84 MRR30 pKa = 11.84 TGNTIRR36 pKa = 11.84 YY37 pKa = 5.79 NAKK40 pKa = 8.47 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.1 WRR45 pKa = 11.84 RR46 pKa = 11.84 TKK48 pKa = 10.89 LKK50 pKa = 10.55 LL51 pKa = 3.39
Molecular weight: 6.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.398
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.135
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.013
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14375
5021
19396
11249408
34
19577
580.0
65.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.091 ± 0.026
1.994 ± 0.027
5.652 ± 0.014
6.732 ± 0.024
3.478 ± 0.014
5.53 ± 0.023
2.521 ± 0.009
5.242 ± 0.017
6.434 ± 0.032
8.881 ± 0.028
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.176 ± 0.01
4.832 ± 0.018
5.557 ± 0.025
3.996 ± 0.018
5.73 ± 0.021
7.709 ± 0.022
5.842 ± 0.019
6.349 ± 0.017
1.092 ± 0.007
3.158 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here