Flexistipes sinusarabici (strain ATCC 49648 / DSM 4947 / MAS 10)

Taxonomy: cellular organisms; Bacteria; Deferribacteres; Deferribacteres; Deferribacterales; Deferribacteraceae; Flexistipes; Flexistipes sinusarabici

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2185 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8E9I1|F8E9I1_FLESM Uncharacterized protein OS=Flexistipes sinusarabici (strain ATCC 49648 / DSM 4947 / MAS 10) OX=717231 GN=Flexsi_1662 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 10.39RR3 pKa = 11.84FILGLFIIPFVDD15 pKa = 3.19ACGGSVSDD23 pKa = 4.57GNSGSSFEE31 pKa = 4.15SARR34 pKa = 11.84VYY36 pKa = 11.1VSGSSIEE43 pKa = 4.12GGAYY47 pKa = 10.32DD48 pKa = 4.97ADD50 pKa = 4.16SVDD53 pKa = 4.5KK54 pKa = 11.47GSGSCDD60 pKa = 2.9NVSNYY65 pKa = 8.92TFTPDD70 pKa = 4.91DD71 pKa = 3.03ITVNVKK77 pKa = 10.35SEE79 pKa = 4.22ALPNLPSDD87 pKa = 4.11LEE89 pKa = 4.08LAEE92 pKa = 4.15VEE94 pKa = 4.56VYY96 pKa = 10.55KK97 pKa = 10.06ITVSFTPNDD106 pKa = 4.53DD107 pKa = 3.37ISPEE111 pKa = 4.08LSTKK115 pKa = 9.83EE116 pKa = 4.2YY117 pKa = 10.61YY118 pKa = 9.24PVSYY122 pKa = 9.97IIEE125 pKa = 4.45PNSTKK130 pKa = 10.52NIPVRR135 pKa = 11.84IIDD138 pKa = 4.49RR139 pKa = 11.84IEE141 pKa = 4.05KK142 pKa = 10.0VDD144 pKa = 3.9NEE146 pKa = 4.35SPLYY150 pKa = 10.76FFDD153 pKa = 4.93NLGKK157 pKa = 10.38EE158 pKa = 4.14YY159 pKa = 10.58NYY161 pKa = 10.13TVNIEE166 pKa = 4.4FKK168 pKa = 10.93AEE170 pKa = 3.7EE171 pKa = 4.25LLYY174 pKa = 11.1GGTDD178 pKa = 3.1SLSYY182 pKa = 10.71SFQLRR187 pKa = 11.84YY188 pKa = 9.5FDD190 pKa = 5.47EE191 pKa = 5.61VDD193 pKa = 3.36DD194 pKa = 4.24CTT196 pKa = 4.59

Molecular weight: 21.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8E5M1|F8E5M1_FLESM Phosphotransferase system phosphocarrier protein HPr OS=Flexistipes sinusarabici (strain ATCC 49648 / DSM 4947 / MAS 10) OX=717231 GN=Flexsi_0993 PE=4 SV=1
MM1 pKa = 7.08SQIRR5 pKa = 11.84TMKK8 pKa = 10.47KK9 pKa = 6.99PTNIKK14 pKa = 9.95KK15 pKa = 9.89KK16 pKa = 9.41RR17 pKa = 11.84KK18 pKa = 7.69QGFRR22 pKa = 11.84ARR24 pKa = 11.84MKK26 pKa = 10.11TRR28 pKa = 11.84GGKK31 pKa = 9.0QVLSRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84AKK40 pKa = 9.98GRR42 pKa = 11.84KK43 pKa = 8.8RR44 pKa = 11.84LAII47 pKa = 4.0

Molecular weight: 5.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2185

0

2185

690808

32

2009

316.2

35.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.13 ± 0.044

1.109 ± 0.024

5.629 ± 0.041

7.241 ± 0.051

5.281 ± 0.049

6.592 ± 0.048

1.672 ± 0.022

8.342 ± 0.052

8.659 ± 0.05

9.199 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.5 ± 0.024

5.374 ± 0.045

3.247 ± 0.025

2.364 ± 0.021

3.951 ± 0.036

6.546 ± 0.039

4.665 ± 0.03

6.666 ± 0.043

0.707 ± 0.014

4.126 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski